Smoothelin (SMTN) (NM_134269) Human Untagged Clone
CAT＃: SC109769
SMTN (untagged)-Human smoothelin (SMTN), transcript variant 2
CNY 6712.00
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_134269, the custom clone sequence may differ by one or more nucleotides
ATGGCGGACGAGGCCTTAGCTGGGCTGGATGAGGGAGCCCTTCGGAAGCTGCTGGAGGTCACAGCAGATC TGGCAGAGCGGCGGCGCATCCGCTCAGCCATCCGGGAACTGCAGCGGCAGGAGCTGGAGCGCGAGGAGGA GGCCCTGGCATCCAAGCGTTTCCGTGCCGAGCGGCAGGACAACAAGGAGAACTGGCTGCACTCTCAGCAG CGGGAAGCTGAGCAGCGGGCTGCCCTGGCACGGCTGGCAGGGCAGCTGGAGTCCATGAACGATGTGGAGG AATTGACTGCACTGTTGCGAAGCGCTGGTGAGTATGAGGAGCGCAAGCTGATCCGAGCTGCCATCCGCCG TGTACGGGCTCAGGAGATTGAGGCTGCCACCTTGGCTGGGAGGTTGTACAGCGGGCGTCCCAACAGTGGC TCAAGAGAGGACAGCAAGGGGCTAGCGGCACACAGGCTGGAACAGTGTGAGGTGCCAGAGCGAGAGGAAC AGGAACAGCAGGCAGAGGTTTCAAAGCCAACCCCCACCCCTGAAGGCACCAGCCAGGATGTGACCACAGT GACACTCCTGCTGCGAGCCCCACCTGGGAGCACATCCAGCTCACCTGCCTCACCCAGCAGTTCACCCACC CCTGCCTCTCCTGAGCCTCCATTGGAGCCTGCCGAGGCCCAGTGCCTTACAGCTGAGGTTCCAGGCAGCC CAGAGCCACCCCCCAGCCCACCCAAGACCACCAGCCCTGAGCCTCAGGAGTCTCCAACGCTCCCCAGCAC TGAGGGCCAGGTGGTCAACAAGCTTCTGTCTGGCCCCAAAGAGACCCCTGCTGCCCAGAGCCCCACCAGA GGCCCCTCTGACACCAAGAGAGCAGACGTGGCTGGACCCCGACCCTGCCAACGCTCCCTGTCGGTGCTCA GCCCCCGCCAACCAGCCCAGAACCGAGAGTCCACCCCCCTTGCCAGCGGACCTTCCTCATTCCAGCGGGC TGGCTCTGTGCGGGATCGTGTCCACAAGTTCACATCTGATTCTCCTATGGCTGCTAGGCTCCAGGATGGC ACACCCCAGGCTGCCCTAAGTCCCCTGACCCCCGCAAGGCTCCTGGGCCCCTCCCTCACCAGCACCACCC CTGCCTCCTCCTCCAGCGGCTCCTCCTCTCGGGGCCCCAGTGATACCTCCTCCCGGTTCAGCAAGGAGCA ACGAGGAGTAGCCCAGCCCCTGGCCCAGCTTCGAAGCTGCCCCCAGGAGGAGGGCCCCAGGGGGCGGGGC TTGGCTGCTAGGCCCCTTGAAAACAGAGCAGGGGGGCCTGTGGCACGTTCAGAGGAGCCTGGTGCCCCGC TGCCCGTGGCCGTCGGCACTGCCGAGCCAGGGGGCAGTATGAAGACCACATTCACCATCGAGATCAAGGA CGGCCGTGGCCAGGCCTCCACAGGCCGGGTGCTGCTGCCCACAGGCAACCAGAGGGCAGAACTGACACTG GGGCTGCGGGCGCCCCCGACCCTACTCAGCACCAGTAGTGGGGGCAAGAGCACCATCACCCGTGTCAACA GCCCTGGGACCCTGGCTCGGCTGGGCAGTGTCACTCATGTCACCAGCTTCAGCCATGCCCCCCCCAGTAG CCGAGGAGGCTGCAGCATCAAGATGGAAGCAGAGCCAGCAGAGCCTCTCGCTGCAGCAGTGGAAGCGGCC AATGGGGCTGAGCAGACCCGAGTGAACAAAGCACCAGAAGGGCGGAGCCCTCTGAGCGCTGAGGAGCTGA TGACTATTGAGGATGAAGGAGTCTTGGACAAGATGCTGGATCAGAGCACGGACTTTGAAGAGCGGAAGCT CATCCGGGCTGCACTTCGTGAGCTCCGACAAAGGAAGAGAGACCAGCGGGACAAGGAGCGGGAACGGCGG CTGCAGGAGGCACGGGGCCGGCCAGGGGAGGGGCGCGGCAACACAGCCACTGAGACCACCACGAGGCACA GCCAGCGGGCAGCTGATGGCTCTGCTGTCAGCACTGTTACCAAGACTGAGCGGCTCGTCCACTCCAATGA TGGCACACGGACGGCCCGCACCACCACAGTGGAGTCGAGTTTCGTGAGGCGCTCGGAGAATGGCAGTGGC AGCACCATGATGCAAACCAAGACCTTCTCCTCTTCCTCCTCATCCAAGAAGATGGGCAGCATCTTCGACC GCGAGGACCAGGCCAGCCCACGGGCCGGCAGCCTGGCGGCGCTCGAGAAACGGCAGGCCGAGAAGAAGAA AGAGCTGATGAAGGCGCAGAGTCTGCCCAAGACCTCAGCCTCCCAGGCGCGCAAGGCCATGATTGAGAAG CTGGAGAAGGAGGGCGCGGCCGGCAGCCCTGGCGGACCCCGCGCAGCCGTGCAGCGATCCACCAGCTTCG GGGTCCCCAACGCCAACAGCATCAAGCAGATGCTGCTGGACTGGTGTCGAGCCAAGACTCGCGGCTACGA GCACGTCGACATCCAGAACTTCTCCTCCAGCTGGAGTGATGGGATGGCCTTCTGTGCCCTGGTGCACAAC TTCTTCCCTGAGGCCTTCGACTATGGGCAGCTTAGCCCTCAGAACCGACGCCAGAACTTCGAGGTGGCCT TCTCATCTGCGGAGACCCATGCGGACTGCCCGCAGCTCCTGGATACAGAGGACATGGTGCGGCTTCGAGA GCCTGACTGGAAGTGCGTGTACACGTACATCCAGGAATTCTACCGCTGTCTGGTCCAGAAGGGGCTGGTA AAAACCAAAAAGTCCTAA >OriGene 5' read for NM_134269 unedited
TCGAAATATGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACCAGGCTGCCCGT GGCCGTCGGCACTGCCGAGCCAGGGGGCAGTATGAAGACCACATTCACCATCGAGATCAA GGACGGCCGTGGCCAGGCCTCCACAGGCCGGGTGCTGCTGCCCACAGGCAACCAGAGGGC AGAACTGACACTGGGGCTGCGGGCGCCCCCGACCCTACTCAGCACCAGTAGTGGGGGCAA GAGCACCATCACCCGTGTCAACAGCCCTGGGACCCTGGCTCGGCTGGGCAGTGTCACTCA TGTCACCAGCTTCAGCCATGCCCCCCCCAGTAGCCGAGGAGGCTGCAGCATCAAGATGGA AGCAGAGCCAGCAGAGCCTCTCGCTGCAGCAGTGGAAGCGGCCAATGGGGCTGAGCAGAC CCGAGTGAACAAAGCACCAGAAGGGCGGAGCCCTCTGAGCGCTGAGGAGCTGATGACTAT TGAGGATGAAGGAGTCTTGGACAAGATGCTGGATCAGAGCACGGACTTTGAAGAGCGGAA GCTCATCCGGGCTGCACTTCGTGAGCTCCGACAAAGGAAGAGAGACCAGCGGGACAAGGA GCGGGAACGGCGGCTGCAGGAGGCACGGGGCCCGGCCAGGGGAGGGGCGCGGGCACACAG CCACTGAGACCACCACGAGGCACAGCCAGCGGGCAGCTGATGGCTCTGCTGTCAGCACTG TTACCAAGACTGAGCGGCTCGTCCACTCCAATGATGGCACACGGACGGCCNGCACCACCA CAGTGGAGTCGAGTTTCGTGAGGCGCTCGGAGAATGCCAGTGGCAGCACCATGATGCCAA CCAAGACCTTNCTCTCTTCCTNCTCATNCAAGAAGATGGGCAGCATCTTCGACCGCGAGG ACAGGCCAGCCCAGGNCCGGCAGCCTGGCGGCGCTCGAGAAACGCAGCCCGAAANAAAAA GAGCTGATGAAGCGCAAN >OriGene 3' read for NM_134269 unedited
NTTGCTCTGGACCGCGGNNCCGCATNCTAGNGATCGAGTTTTTTTTTTTTTTTTTTTTTT TTTTTTCGGGGAAAACCAATAATTTATCAAAACCCTGCGTGTGTATGTGGGGGGGAGGGT GTCCCAACAAACAGGGCAGCGGTGGGCGGACCCACAGGCAGGAAACGGTGCCCGGAAAGT GGGGGCGGCAGCTTGCCACTGGCTGGCCATGCGGGCGGGCAGGCTAAACATTCTTGCCGC GCAGGCGCAGTTCGTGGCGTCGCAGGTGGTTGTAAAGCGACTGCACATAGGTGAAGACAC ACTTGGGGTCAGGCTTCTTGCCCATGATCATCATGTCGTCCACCTCCACCAGGGGCACAC AGTCCACCAGCATCTCCGCAGATGAAAAGGCCACCTCGAAGTTCTGGCGTCGGTTCTGAG GGCTAACCTGCCCATAGTCGAAGGCCTCAGGGAAAAAGTTGTGCACCAGGGCCCAGAAGG CCATCCCATCACTCCAACTGGAGGAACAATCCTGGCTCCCCCCTCCTCGCACCCCCGATT TTTGGTTCGACCCCATTCCCCACCAACTGCTTGCACCTGTTACCCTTGCGCCCCCCAACC CTCCGCTGTCCTTCCCCGCCCCCCCGGTCCCTCCCGCCGTTCTTCCCCCTCCCCTTCCCC CCGCTCCGTCTGTGTTCCCCCCCTCTCTCTCTCCTTACCCCGCCTTATCCTCCTCTCTCC TTCGCCCCCCTGCTCTTTTTTCCCTCTTTCCCCTCCTTCTCGTTTTTTTGCCCTCCCTGT CTTCCCTCGCTCGCTCCCTGTGCCCCTCATCTTTTCCCCGCCCCTTTAATCCCTCCNCTT TTTTATCCCTCCTCGCCTCTCCTTCCCCCTCGCTATCCTCCTCCTCACCTTCCCCTCTCC TCCTTTCGCCCTCCGTTATCCCTCTTCGCACCGTACACG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_134269 |
| Insert Size | 1600 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_134269.1, NP_599031.1 |
| RefSeq Size | 3294 bp |
| RefSeq ORF | 2748 bp |
| Locus ID | 6525 |
| UniProt ID | P53814 |
| Domains | CH |
| Gene Summary | This gene encodes a structural protein that is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. This gene is localized to chromosome 22q12.3, distal to the TUPLE1 locus and outside the DiGeorge syndrome deletion. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2011] Transcript Variant: This variant (2) has an alternate 5' exon, which results in a downstream AUG start codon, lacks an in-frame exon in the middle region and has an additional exon in the 3' region, compared to variant 4. The resulting isoform (b, also referred to as L2 and B2) is shorter at the N-terminus, lacks a segment in the middle region and has a slightly shorter and distinct C-terminus, compared to isoform d. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202191 | SMTN (Myc-DDK-tagged)-Human smoothelin (SMTN), transcript variant 2 |
CNY 6704.00 |
|
| RC202191L3 | Lenti ORF clone of Human smoothelin (SMTN), transcript variant 2, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC202191L4 | Lenti ORF clone of Human smoothelin (SMTN), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RG202191 | SMTN (tGFP-tagged) - Human smoothelin (SMTN), transcript variant 2 |
CNY 8304.00 |
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