Endothelin 3 (EDN3) (NM_000114) Human Untagged Clone
CAT＃: SC110001
EDN3 (untagged)-Human endothelin 3 (EDN3), transcript variant 1
CNY 2400.00
CNY 3990.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ET-3; ET3; HSCR4; PPET3; WS4B |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC110001 sequence for NM_000114 edited (data generated by NextGen Sequencing)
ATGGAGCCGGGGCTGTGGCTCCTTTTCGGGCTCACAGTGACCTCCGCCGCAGGATTCGTG CCTTGCTCCCAGTCTGGGGATGCTGGCAGGCGCGGCGTGTCCCAGGCCCCCACTGCAGCC AGATCTGAGGGGGACTGTGAAGAGACTGTGGCTGGCCCTGGCGAGGAGACTGTGGCTGGC CCTGGCGAGGGGACTGTGGCCCCGACAGCACTGCAGGGTCCAAGCCCTGGAAGCCCTGGG CAGGAGCAGGCGGCCGAGGGGGCCCCTGAGCACCACCGATCCAGGCGCTGCACGTGCTTC ACCTACAAGGACAAGGAGTGTGTCTACTATTGCCACCTGGACATCATTTGGATCAACACT CCCGAACAGACGGTGCCCTATGGACTGTCCAACTACAGAGGAAGCTTCCGGGGCAAGAGG TCTGCGGGGCCACTTCCAGGGAATCTGCAGCTCTCACATCGGCCACACTTGCGCTGCGCT TGTGTGGGGAGATATGACAAGGCCTGCCTGCACTTTTGCACCCAAACTCTGGACGTCAGC AGTAATTCAAGGACGGCAGAAAAAACAGACAAAGAAGAGGAAGGGAAGGTTGAAGTCAAG GACCAACAAAGCAAGCAGGCTTTAGACCTCCACCATCCAAAGCTCATGCCCGGCAGTGGA CTCGCCCTCGCTCCATCTACCTGCCCCCGCTGCCTCTTTCAGGAAGGAGCCCCTTAG Clone variation with respect to NM_000114.2 >OriGene 5' read for NM_000114 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGTCCTCCTGGTCCGG TGCTCCGGCGCCTGATCTAGGTTCATGGAGCCGGGGCTGTGGCTCCTTTTCGGGCTCACA GTGACCTCCGCCGCAGGATTCGTGCCTTGCTCCCAGTCTGGGGATGCTGGCAGGCGCGGC GTGTCCCAGGCCCCCACTGCAGCCAGATCTGAGGGGGACTGTGAAGAGACTGTGGCTGGC CCTGGCGAGGAGACTGTGGCTGGCCCTGGCGAGGGGACTGTGGCCCCGACAGCACTGCAG GGTCCAAGCCCTGGAAGCCCTGGGCAGGAGCAGGCGGCCGAGGGGGCCCCTGAGCACCAC CGATCCAGGCGCTGCACGTGCTTCACCTACAAGGACAAGGAGTGTGTCTACTATTGCCAC CTGGACATCATTTGGATCAACACTCCCGAACAGACGGTGCCCTATGGACTGTCCAACTAC AGAGGAAGCTTCCGGGGCAAGAGGTCTGCGGGGCCACTTCCAGGGAATCTGCAGCTCTCA CATCGGCCACACTTGCGCTGCGCTTGTGTGGGGAGATATGACAAGGCCTGCCTGCACTTT TGCACCCAAACTCTGGACGTCAGCAGTAATTCAAGGACGGCAGAAAAAACAGACAAAGAA GAGGAAGGGAAGACAGGTTGAAGTCAAGGACCAACATAGCAAGCAGGCTNNTAGACCTNC ACCATCCAAAGCTCATGCCCGGCAGTGGACTCGCCCTCGCTCCATCTACCTGCCCNCGCT GCCTCTTNCAAGGAAGGAGCCNNCTTAGAGGACAGGCCTGCAGCATCCTGGTCTCGGGGA GGCTTCTGTCATTGCTCACAACAGTTCAGATTTCCACTCTTTTAGACAGAAGTGATTGCT GGGCGCGGCAA >OriGene 3' read for NM_000114 unedited
TAGCTTTGGACCGGCGGCCGCAATCTAGGATCGAGGTTTTTTTTTTTTTTTTTTTTTTTT TTTCAAAACTTTAGGTTTTTTATTAGGCTTTTGACAGTACTTTCTGAGTATTTGAGCAAT TGAGGGGTTTCTCTTTGATACCATCTTGCCACGGGGCCAAACAGCACATATGCATTTTAA AAATGTAATTCAAGCTCTAAGGAGTATGAGTTTGTATTCAAAATGCAATTTAAAATCTAC CTACCTATCTACCTCTTTATACGTATATACAGGGACTACTTTGAACAGATCCACATAAAG GAGTTGGAGGGGAAAACAGTTTCACAATAAATAATCGTTTCTTTAAACTGTACAAAATCC TACCAGCAAATCAGCCACTTTCATTTCCTCATGATAGGTCATCACTTTTATACCTGTAAT ACCTTATGAAATACACACAGAGGATTTAACATTTGGTCAAGGCCAATGCTCTGTCATACA CCATCTTAAATGTCTCTCTACGGGGCCCTGGACACCCACTCAAATGCCGTTTCCTATCTC TCTGTGACTGGGTTGGGGAGATGGGCAGCCCTCCCCTGCGCAGGCGACTTTACATGGGGG GATAACATCTCCACACTCACAGTAAGCTCGGCTCGGTGGGAAAAGCGCAGCTGAAAGAGA TTTTGAACTGTCAAACGGTATAAGTTGTTGCCAATTTATATAAACAGGAATTTACATAAT TTACAAAGAATTTGTGTTCGTGACTTATTTTGAAGCCATTCCCCAAAGATCCACATTTAC TCATGAAAGCAAATATTCAACCTAATAGCCTTGAGCTGNGGCTGTCTTTAAGTCGGCTCC TGCAAACAGGCTGNGAACTCACTGTGTATCGCCTGTGCCTATCAGATACAGAGGTCAGCC CTGACAGAACAGCCTGNATGCAGTCTACTTCTTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000114 |
| Insert Size | 2440 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000114.2, NP_000105.1 |
| RefSeq Size | 2438 bp |
| RefSeq ORF | 717 bp |
| Locus ID | 1908 |
| Domains | END |
| Protein Families | Druggable Genome, Secreted Protein |
| Gene Summary | The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (1) encodes the longest isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220053 | EDN3 (Myc-DDK-tagged)-Human endothelin 3 (EDN3), transcript variant 1 |
CNY 2400.00 |
|
| RC220053L1 | Lenti ORF clone of Human endothelin 3 (EDN3), transcript variant 1, Myc-DDK-tagged |
CNY 4800.00 |
|
| RC220053L2 | Lenti ORF clone of Human endothelin 3 (EDN3), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RC220053L3 | Lenti ORF clone of Human endothelin 3 (EDN3), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC220053L4 | Lenti ORF clone of Human endothelin 3 (EDN3), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG220053 | EDN3 (tGFP-tagged) - Human endothelin 3 (EDN3), transcript variant 1 |
CNY 4370.00 |
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