RNF22 (TRIM3) (NM_006458) Human Untagged Clone
CAT＃: SC110176
TRIM3 (untagged)-Human tripartite motif containing 3 (TRIM3), transcript variant 1
CNY 5456.00
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BERP; HAC1; RNF22; RNF97 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC110176 sequence for NM_006458 edited (data generated by NextGen Sequencing)
ATGGCAAAGAGGGAGGACAGCCCTGGCCCAGAGGTCCAGCCAATGGACAAGCAGTTCCTG GTATGCAGCATCTGCCTGGATCGGTACCAGTGCCCCAAGGTTCTTCCTTGCCTGCACACC TTCTGTGAGAGATGTCTCCAAAACTATATCCCTGCCCAGAGCCTGACGCTATCCTGTCCA GTATGCCGGCAGACGTCCATCCTCCCAGAGCAGGGCGTCTCGGCACTGCAGAACAACTTC TTCATCAGCAGCCTCATGGAGGCAATGCAGCAGGCACCTGATGGGGCCCACGACCCGGAG GACCCCCACCCCCTCAGTGTAGTGGCTGGCCGCCCTCTCTCCTGCCCCAACCATGAAGGC AAGACGATGGAGTTTTACTGTGAGGCCTGTGAGACGGCCATGTGTGGTGAGTGCCGCGCC GGGGAGCATCGTGAGCATGGCACAGTGCTGCTGAGGGATGTGGTGGAGCAGCACAAGGCG GCCCTGCAGCGCCAGCTCGAGGCTGTGCGTGGCCGATTGCCACAGCTGTCCGCAGCAATT GCCTTAGTCGGGGGCATCAGCCAGCAGCTGCAGGAGCGCAAGGCAGAGGCCCTGGCCCAG ATCAGTGCAGCGTTCGAGGACCTGGAGCAAGCACTGCAGCAGCGCAAGCAGGCTCTGGTC AGCGACCTGGAGACCATTTGTGGGGCCAAACAGAAGGTGTTGCAAAGCCAGCTGGACACA CTGCGCCAGGGTCAGGAACACATCGGCAGTAGCTGCAGCTTTGCAGAGCAGGCACTGCGC CTGGGCTCGGCCCCGGAGGTGTTGCTGGTGCGCAAGCACATGCGAGAGCGGCTGGCTGCA TTGGCGGCACAGGCCTTCCCGGAGCGGCCACATGAGAATGCACAGCTGGAACTGGTCCTT GAGGTGGACGGTCTGCGGCGATCGGTGCTCAATCTGGGCGCACTGCTCACCACGAGCGCC ACTGCACACGAAACGGTGGCCACGGGAGAGGGCCTGCGCCAGGCGCTAGTGGGCCAGCCT GCCTCGCTCACTGTCACTACCAAAGACAAGGACGGGCGGTTGGTGCGCACAGGCAGCGCT GAGCTGCGTGCAGAGATCACCGGCCCGGACGGCACGCGCCTTCCGGTGCCAGTGGTGGAC CACAAGAATGGCACATATGAGCTAGTGTACACAGCGCGCACGGAAGGCGAGCTGCTCCTC TCGGTGCTGCTCTACGGACAGCCAGTGCGCGGCAGCCCCTTCCGCGTGCGTGCCCTGCGT CCGGGGGACCTGCCACCTTCCCCGGACGATGTGAAGCGCCGTGTCAAGTCCCCTGGCGGC CCCGGCAGCCATGTGCGCCAGAAGGCAGTGCGTAGGCCCAGCTCCATGTACAGCACAGGC GGCAAACGAAAGGACAACCCAATTGAGGATGAGCTCGTCTTCCGTGTTGGCAGTCGTGGA AGGGAGAAAGGTGAATTCACCAATTTACAAGGTGTGTCCGCAGCCAGCAGCGGCCGCATC GTGGTAGCAGACAGCAACAACCAGTGTATTCAGGTTTTCTCCAATGAGGGCCAGTTCAAG TTCCGTTTTGGGGTCCGAGGACGCTCACCTGGGCAGCTGCAGCGCCCCACAGGTGTGGCA GTGGACACCAATGGAGACATAATTGTGGCAGACTATGACAACCGTTGGGTCAGCATCTTC TCCCCTGAGGGCAAGTTCAAGACCAAGATTGGAGCTGGCCGCCTCATGGGCCCCAAGGGA GTGGCCGTAGACCGGAATGGACATATCATTGTGGTCGACAACAAGTCTTGCTGCGTCTTT ACCTTCCAGCCCAATGGCAAACTGGTTGGCCGTTTTGGGGGCCGTGGGGCCACTGACCGC CACTTTGCAGGGCCCCATTTTGTGGCTGTGAACAACAAGAATGAAATTGTAGTAACGGAC TTCCATAACCATTCAGTGAAGGTGTACAGTGCCGATGGAGAGTTCCTCTTCAAGTTTGGC TCCCATGGCGAGGGCAATGGGCAGTTCAATGCCCCCACAGGAGTAGCTGTGGACTCCAAT GGAAACATCATTGTGGCTGACTGGGGCAACAGCCGCATCCAGGTATTCGACAGCTCTGGC TCCTTCCTGTCCTATATCAACACATCTGCAGAACCACTGTATGGTCCACAGGGCCTGGCA CTGACCTCGGATGGCCATGTGGTGGTGGCTGATGCTGGCAACCACTGCTTTAAAGCCTAT CGCTACCTCCAGTAG Clone variation with respect to NM_006458.2 >OriGene 5' read for NM_006458 unedited
GACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGAGGCTGGTGTGAGTGGCGGGA GCCATCTGTGGGCGCCATGGCAAAGAGGGAGGACAGCCCTGGCCCAGAGGTCCAGCCAAT GGACAAGCAGTTCCTGGTATGCAGCATCTGCCTGGATCGGTACCAGTGCCCCAAGGTTCT TCCTTGCCTGCACACCTTCTGTGAGAGATGTCTCCAAAACTATATCCCTGCCCAGAGCCT GACGCTATCCTGTCCAGTATGCCGGCAGACGTCCATCCTCCCAGAGCAGGGCGTCTCGGC ACTGCAGAACAACTTCTTCATCAGCAGCCTCATGGAGGCAATGCAGCAGGCACCTGATGG GGCCCACGACCCGGAGGACCCCCACCCCCTCAGTGTAGTGGCTGGCCGCCCTCTCTCCTG CCCCAACCATGAAGGCAAGACGATGGAGTTCTACTGTGAGGCCTGTGACACGGCCATGTG TGGTGAGTGCCGCGCCGGNGAGCATCGTGAGCATGGCACAGTGCTGCTGANGGATGTGGT GGAGCAGCACAAGGCGGCCCTGCATCGCCAGCTCGAGGCTGTGCGTGGCCGATTGCCACA GCTGTCCGCAACAATTGCCTTAGTCGGNGGCATCANCCANCANCTGCATGAGCGCAAGGC AAAGGCCCNTGCCCAGATCAGTGCANCGTTCGAGGACCTGAAGCAAGCACTGCATCAGCG CAAGCAGGCTCTGGTCANCGACCTGGAGCCATTTGTGGGGACAAACAGAATGTGTTGCAA GCCAGCTGGACCACTGCGCCAGGTCANGACACATCGGCAGTAGCTGCACTTTGCAAAGCA GCACTGCGCCTGGCTCGCCCCGGAGTTGTGCTGTTGCCAGCCATGAANAGCGCTGCCTGA TTGCGGACAGCTTCCCGACGNCAATGAAATGCACTGGACTGTCCTGAGNAGACGCTGGCA ATCGTGCTATCTGC >OriGene 3' read for NM_006458 unedited
AATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTTGGTGAAGACATTTATTTAATATGGG GGGTGGGGTATTGCTGTCTCTGTCAGTGTGTATAGGGTGGTAGGGAGACAACTAGAGGGA CTCCTGTCCTGGGGTAGGCTGTTCTGGCCCCAGGCTGGGGGATGGGGAGCAGACTGACAG GGGTGGGGAGGTGTGTAAAAAGGGTAGGGTGAAGCTCTGCACACATCCTTGGGACCACAG TCCAGGCTCACCCAGTCACCAAAGCAAGAACCGAATAAATAAAGTGCAACCGTGGGGGTG GGGGTAGGAGAGGGAGGGCACCTGTTGCACCCATGCCCACAGCCCACATTCAGTGCTGCC AGGACTGGCCCACCTACCATCCATACCCTTTTGTACAATTTCCCCAATGTGGGTCCTTCC ACAAGCCATGCAAGGCCTCTGTACAGTTACTGGAGGCATCGATTGGCTTTAAAGCACTGG TGTGGCAGAATCTGCCACCACCAGATGTCCATGCGACGTTAGTGCCAGGCCCTGCGGACA TACAGGGGCANTCTAGATGTGTATGTACATGACTGGAACGTTCCAGGACTGTCACATACT TTGGTTCTGTTGCTGGCCGATGCACTCAGGATGAAAGGGCCCTTGGAGTCCACAACTACT TGTGGTGTGGAGAGCCACTTGCAATTGCTCTCGCCATGGGATGCCCCTTTGAAAAGAATT TTTCGCCTACTTTGTACCACTTTAGGGAAGGGTTTTTCCTTTCGCGGGATGCAGCTCTTT TTTGGTATGGACCCCCTCCTAAAAGAGGTCCTCCAAGGAGCCTAGCCACACCCCACCGGT GCGGAATGCGGCAACCAGCTGGGAACTGGGTAATATGTTTAAAGACGTTCTTTTTTTTTG ACCGGGAATTCCTTTCAAATCTGAGCCCACGCCCCCTCTTGGGGCAATAAGCCACAAGAC ACTTCACATAATTGACTTATATCTTGAGCTAAACACTTCATTCTTTGATATTGGGCCAAT GTGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006458 |
| Insert Size | 2840 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006458.2, NP_006449.2 |
| RefSeq Size | 3059 bp |
| RefSeq ORF | 2235 bp |
| Locus ID | 10612 |
| UniProt ID | O75382 |
| Domains | zf-B_box, NHL, Filamin, RING, BBC |
| Gene Summary | The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 4 encode the same protein (isoform 1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211739 | TRIM3 (Myc-DDK-tagged)-Human tripartite motif containing 3 (TRIM3), transcript variant 1 |
CNY 5448.00 |
|
| RC211739L1 | Lenti ORF clone of Human tripartite motif containing 3 (TRIM3), transcript variant 1, Myc-DDK-tagged |
CNY 7848.00 |
|
| RC211739L2 | Lenti ORF clone of Human tripartite motif containing 3 (TRIM3), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RC211739L3 | Lenti ORF clone of Human tripartite motif containing 3 (TRIM3), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC211739L4 | Lenti ORF clone of Human tripartite motif containing 3 (TRIM3), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG211739 | TRIM3 (tGFP-tagged) - Human tripartite motif containing 3 (TRIM3), transcript variant 1 |
CNY 7048.00 |
