PIGN (NM_012327) Human Untagged Clone
CAT＃: SC110315
PIGN (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 2
CNY 6824.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MCAHS; MCAHS1; MCD4; MDC4; PIG-N |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>SC110315 representing NM_012327.
Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCTGCTGTTCTTTACTTTGGGATTGCTTATACATTTTGTGTTCTTCGCCTCCATCTTTGACATTTAT TTTACATCTCCTTTGGTTCATGGAATGACTCCTCAGTTTACACCATTGCCTCCTCCAGCGAGAAGATTA GTGTTGTTTGTTGCTGATGGCCTTCGAGCAGATGCACTTTACGAATTAGATGAAAATGGAAACTCTAGA GCACCGTTTATTAGGAATATCATAATGCATGAAGGCAGCTGGGGCATATCTCATACACGTGTGCCAACA GAATCTCGGCCAGGTCATGTAGCTCTGATAGCTGGGTTTTATGAAGATGTCAGTGCAGTTGCCAAAGGA TGGAAGGAAAATCCTGTAGAGTTTGATTCTCTTTTTAATGAAAGTAAATACACATGGAGCTGGGGAAGC CCAGATATCCTGCCTATGTTTGCCAAAGGTGCTAGTGGAGACCACGTTTATACATATAGTTATGATGCT AAAAGAGAGGATTTTGGTGCTCAAGATGCAACAAAACTGGATACGTGGGTTTTTGATAATGTTAAGGAC TTCTTTCATCATGCCAGAAACAACCAGTCTTTGTTTTCTAAAATAAATGAAGAGAAAATAGTTTTTTTC TTACATTTATTAGGAATAGATACAAACGGACATGCTCATCGACCATCCTCGAGAGACTACAAGGACAAT ATTAAAAAAGTTGATGATGGAGTTAAAGAAATCGTGTCTATGTTTAACCATTTCTATGGAAATGATGGG AAAACAACATTTATCTTTACCTCTGACCATGGAATGACAGACTGGGGTTCCCATGGGGCTGGTCATCCT TCAGAGACTTTAACTCCTTTAGTCACTTGGGGAGCTGGAATCAAGTATCCCCAAAGAGTATCAGCTCAG CAATTTGATGATGCATTTTTGAAAGAGTGGAGATTGGAGAACTGGAAGAGGCTAGATGTCAATCAGGCT GATATTGCACCATTGATGACTTCCCTTATTGGAGTTCCCTTTCCTCTTAACTCAGTGGGAATCCTTCCT GTGGATTATCTTAACAACACTGATCTCTTCAAAGCAGAGAGCATGTTTACAAATGCAGTACAGATTCTT GAACAGTTCAAGGTGAAAATGACTCAGAAGAAAGAAGTTACTTTACCATTTTTGTTTACACCATTTAAA CTGCTTTCTGATTCCAAACAGTTCAACATTTTAAGAAAAGCAAGATCTTATATAAAACACAGAAAGTTT GACGAAGTGGTCTCCCTTTGCAAGGAGCTAATTCATCTTGCATTGAAAGGATTGTCCTATTATCACACA TATGACAGATTCTTTTTGGGCGTCAATGTTGTTATTGGTTTTGTGGGATGGATATCTTATGCCTCCTTG TTGATCATCAAGTCTCATTCCAACCTTATAAAAGGTGTTAGTAAAGAAGTGAAGAAACCAAGCCATCTC CTGCCTTGTAGTTTTGTAGCTATTGGCATTTTAGTAGCATTTTTTCTGCTGATTCAAGCCTGTCCCTGG ACATATTATGTATATGGTTTGTTGCCACTGCCAATATGGTATGCGGTTCTAAGAGAATTTCAAGTTATT CAGGACCTTGTTGTATCAGTGTTGACCTATCCTCTGAGCCATTTTGTTGGGTACCTGTTAGCCTTTACC CTGGGAATTGAAGTATTAGTTCTCAGTTTTTTCTACCGCTATATGCTTACCGCTGGACTTACTGCCTTT GCAGCTTGGCCATTTCTCACTCGGCTATGGACTCGAGCAAAGATGACCTCACTGAGTTGGACTTTCTTC TCTTTGCTCCTGGCAGTGTTCCCACTGATGCCGGTTGTAGGTCGAAAGCCAGACATCTTTCTAGTGATG GGTGCAGGCTTGCTGGTTCTTCTGTTATCCCTGTGTGTTGTAACATCTCTCATGAAAAGAAAAGATAGC TTTATAAAGGAAGAGCTATTGGTACATCTATTACAGGTGCTGAGCACAGTGCTCTCCATGTATGTTGTG TATAGCACCCAGAGTAGTCTACTCAGGAAGCAAGGACTGCCTCTCATGAATCAAATTATTAGCTGGGCA ACATTAGCCTCTTCCTTGGTTGTGCCACTACTGAGTTCTCCAGTTCTCTTTCAGCGATTGTTCAGCATA CTTCTTTCATTGATGTCAACCTACCTACTTCTAAGCACAGGGTATGAAGCTCTCTTTCCACTAGTGTTG TCTTGTTTGATGTTTGTCTGGATAAACATAGAACAAGAAACTCTACAACAATCTGGTGTTTGCTGTAAA CAAAAGCTCACCAGTATCCAGTTCTCTTATAATACTGATATAACTCAGTTTCGACAGCTATATCTGGAT GACATCCGTAGGGCCTTTTTCCTTGTTTTCTTCTTAGTGACAGCATTTTTTGGAACTGGAAATATAGCT TCTATTAACAGCTTTGATCTTGCCTCTGTCTATTGCTTTCTGACTGTGTTCAGTCCTTTTATGATGGGA GCCCTGATGATGTGGAAGATTTTAATCCCCTTTGTTCTTGTTATGTGTGCTTTTGAAGCAGTTCAGTTG ACTACTCAGTTATCGTCAAAAAGCCTTTTTCTCATTGTTCTCGTCATATCAGACATTATGGCTTTGCAT TTTTTCTTCTTGGTCAAGGATTATGGCAGCTGGCTTGATATTGGGACAAGCATCAGCCATTATGTGATT GTCATGTCCATGACCATCTTTTTGGTGTTCCTCAATGGCCTGGCCCAGCTGCTCACAACGAAGAAACTC AGACTATGTGGCAAACCCAAAAGTCACTTCATGTGA >OriGene 5' read for NM_012327 unedited
GTCAAATATTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGAATACCCTCC CGGCTATCGCTTCAGTGGGTAGAGGGGACCACTGCCCGAGAGCTTTAATGGAGCTGGGTC CTGCCTTCGCGCTGAGGAGCCCTCGTTTTCGAGATCAGGCCTGACCGGGATAAGCTCCAG CTGTTCTTGAAGACTAATTAGTTACACTGGATATGCTGCTGTTCTTTACTTTGGGATTGC TTATACATTTTGTGTTCTTCGCCTCCATCTTTGACATTTATTTTACATCTCCTTTGGTTC ATGGAATGACTCCTCAGTTTACACCATTGCCTCCTCCAGCGAGAAGATTAGTGTTGTTTG TTGCTGATGGCCTTCGAGCAGATGCACTTTACGAATTAGATGAAAATGGAAACTCTAGAG CACCGTTTATTAGGAATATCATAATGCATGAAGGCAGCTGGGGCATATCTCATACACGTG TGCCAACAGAATCTCGGCCAGGTCATGTAGCTCTGATAGCTGGGTTTTATGAAGATGTCA GTGCAGTTGCCAAAGGATGGAAGGAAAATCCTGTAGAGTTTGATTCTCTTTTTAATGAAA GTAAATACANCATGGAGCTNGGGGAAGCCCAGATATCCTGCCTATGTTTGCCAAAGGTGC TAGTGGAGACCACGTTTATACATATAGTTATGATGCTAAAAGAGAGGATTTTGGTGCTCA AGATGCAACAAACTGGATACCGTGNGTTTTTGANTATGTTAAGGACTTCTTTCATCATGC CAGAAACACCAGTCTTTTGTTTTCTAANATAATGAAGAGAAATAGTTTTTTTTCTACATT TATTAGAATAGATACAACGGACATGCTCATCGACATCCTCGAGAGACTACAGACATATNA AAAAGTTGTGATGGAGTAAGGAATCGGGTCTTGTTTACCATTCTATGGATG >OriGene 3' read for NM_012327 unedited
GGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTGCAAACACTTTTTTTATTGAA GCTCTTGAAAAATACACCCTGACTGTCTAACAAAACCAGTGCTGAAAGCTGATAAATATT GACGGAGGCACCTGGTGATTTCCTTCCAGCTCAGCCACAGCAATGACTCTGGATGCCATC AGCACTGCCCCCACAGGCATATGCTCTCCTCTGGGTATGTGGGAAGTTGTGCGACTGGGG ACCAGATTGCTGCAGGTGCTCTCAACATCACTGGGAAGAGCTGCCAGCCAAAGGAAAAAA ATGAAAAAGGAGAATGCCTTCCTATGCTTTTCCACAGATATAATCACTATTTCATGCCTG CAAAACCTATAAAAAAAAAAGAAGCTCCTTTGTTCCAGATAACCTGTCAATTCGGAATTC CAAATACCATTTTCCTTACAGGAGTAGAATATACTATATATCCATATCCATCTTCTTATT GAAGCCTTGATGAGATTTTAGCTTAAAAGGAGAATCAGGATCCAGATGCTGAATGGTGCT TCAGCAACCTCACATGAAGTGACTTTTGGGTTTGCCACATAGTCTGAGTTTCTTCGTTGT GAGCAGCTGGGCCAGGCCATTGAGGAACACCAAAAAGATGGTCATGGACATGACANTCAC ATAATGGCTGATGCTTGTCCCAATATCAAGCCAGCTGCCATAATCCTTGACCAAGAAGAA AAAATGCACAGCCATAATGCCTGATATGACGAGAACAATGAGAAAAAAGCTCTTTGACGA TAACTGAGTAGTCAACCTGACCTGCCTCATAGCACACCTATCATGACACAAGGGACTAAA ATCCTTCACATTTCACGCCTCCCATCATAAAAGACTGAACACAGTCCGAAGCAATAGACG AAGCCAGATCAAAGCTGTTAATAGAAGCTTATTTCCAGTCCCAAAATGCTGTCACTATAT TAATACAGNATAAGCCCTCACGAGCCTCCAGATAGCTGCGAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_012327 |
| Insert Size | 2796 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012327.3 |
| RefSeq Size | 4751 bp |
| RefSeq ORF | 2796 bp |
| Locus ID | 23556 |
| UniProt ID | O95427 |
| Domains | PigN |
| Protein Families | Transmembrane |
| Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways |
| MW | 105.8 kDa |
| Gene Summary | This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is expressed in the endoplasmic reticulum and transfers phosphoethanolamine (EtNP) to the first mannose of the GPI anchor. Two alternatively spliced variants, which encode an identical isoform, have been reported. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222950 | PIGN (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 2 |
CNY 6816.00 |
|
| RC222950L3 | Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 2, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC222950L4 | Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RG222950 | PIGN (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 2 |
CNY 8416.00 |
