PDK3 (NM_005391) Human Untagged Clone
CAT＃: SC110971
PDK3 (untagged)-Human pyruvate dehydrogenase kinase, isozyme 3 (PDK3), nuclear gene encoding mitochondrial protein, transcript variant 2
CNY 5488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CMTX6; GS1-358P8.4 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_005391 edited
ATGCGGCTGTTCCGGTGGCTGCTGAAGCAGCCGGTGCCCAAGCAGATCGAGCGCTACTCG CGCTTTTCGCCGTCGCCGCTCTCCATCAAACAATTCCTGGACTTCGGGAGAGATAATGCA TGTGAGAAAACTTCATATATGTTTCTACGAAAGGAACTTCCTGTGCGGCTGGCTAACACA ATGAGAGAAGTTAATCTTCTGCCGGATAATTTACTTAACCGCCCTTCAGTGGGATTGGTT CAGAGTTGGTATATGCAGAGTTTTCTTGAACTTTTAGAATATGAAAATAAGAGCCCTGAG GATCCACAGGTCTTGGATAACTTTCTACAAGTTCTGATTAAAGTCAGAAATAGACACAAT GATGTGGTTCCTACAATGGCACAAGGAGTGATTGAATACAAGGAGAAGTTTGGGTTTGAT CCTTTCATTAGCACTAACATCCAATATTTTCTGGATCGGTTTTATACCAACCGCATCTCT TTCCGCATGCTTATTAATCAGCACAGAAGTATCGATCCCACCTGTAACGTGGCGGATGTG GTGAAAGATGCATATGAAACAGCCAAGATGCTGTGTGAACAGTATTACCTGGTAGCTCCA GAGCTGGAAGTTGAAGAATTCAATGCCAAAGCGCCAGACAAACCTATTCAGGTGGTTTAT GTGCCCTCACATCTGTTTCATATGCTATTTGAGTTGTTCAAGAACTCAATGAGAGCGACA GTTGAACTCTATGAAGACAGAAAAGAGGGCTACCCTGCTGTTAAAACCCTCGTTACTTTG GGTAAAGAAGACTTATCCATTAAGATCAGTGACCTAGGTGGTGGTGTCCCACTTCGAAAA ATAGATCGTCTTTTTAACTACATGTATTCTACTGCTCCTAGACCCAGCCTGGAGCCTACC AGAGCTGCCCCTTTGGCTGGATTTGGTTATGGTTTGCCAATTTCCCGTCTGTATGCTAGA TATTTTCAAGGAGATCTGAAACTGTATTCCATGGAAGGAGTGGGTACTGATGCTGTCATT TATTTGAAGGCTCTTTCAAGTGAGTCATTTGAGAGACTTCCAGTTTTTAATAAGTCCGCA TGGCGCCATTACAAGACCACGCCTGAAGCCGATGATTGGAGCAATCCCAGCAGTGAACCC AGGGATGCTTCAAAATACAAAGCAAAACAAGTACAAGATCAAGACTAATAGAACTTTCTA GAGGACTGAATGCTGTGGTCCTCTCTGTGAAGAAAGATTGCCTTCTGCAAGTCAAACAGA GAGAACTGCTTTCTCCCACCTGCTCTGAGCGTGGCATCACAGTTATTCCTAGTGCTTAAA TGTGTATTTTCTCTCTACCACCTGGACTTTCCCAAAGAGTTTTTCCTGTAGCTACTCCAG ATCTTCCACTAAAGTAGTAACTTTTTAATGACATCTTGCTGTGGTGTGATTGATGCCTGT CACCGAAGAGCACAGAGCCTCCTTACAGCTCTTGTGCCCTCGTACTCTCCATCTTGTTAT CATAGCATCTGATGTCTTCAGTCCTTCCAGGATTCTGTGCGCCTTTCCCATTCCAGATAC AGCAGATCGTACTGTTGCTCCTTGTGACCAAATACCATATTTGTCCCAAACCTCCACCTA GGGCCCTGGGACCCTGAAAGTGGAAGACATCAGGTGGCCATGCCCTGATGATTTAGTCCC AGAGCCCTCCGTCTCTCCCGTGCCTCCCTCCGCCACTCTATGATTTAGACTTTTGCCTTC TTCCATCTGATCGGATTCTCATTGTGAGATGTGGTGACTTTCATGTTTA >OriGene 5' read for NM_005391 unedited
CCGCCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC TCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCG GCACGAGTGCGGCGGCTGCACCGGCGGCGCCGAGGCCGAGATCGAGGCCGGGGTGCGCGC TTCGCAAACGTGCCCTATCCGTGCGGCTTGGCTGCGCCAGCCCTTGCGGCCACCCGGGCG TCTAGGCGGGTCTGTGCGCCGCCCGGGCGAGGATGCGGCTGTTCCGGTGGCTGCTGAAGC AGCCGGTGCCCAAGCAGATCGAGCGCTACTCGCGCTTTTCGCCGTCGCCGCTCTCCATCA AACAATTCCTGGACTTCGGGAGAGATAATGCATGTGAGAAAACTTCATATATGTTTCTAC GAAAGGAACTTCCTGTGCGGCTGGCTAACACAATGAGAGAAGTTAATCTTCTGCCGGATA ATTTACTTAACCGCCCTTCAGTGGGATTGGTTCAGAGTTGGTATATGCAGAGTTTTCTTG AACTTTTAGAATATGAAAATAAGAGCCCTGAGGATCCACAGGTCTTGGATAAACTTTCTA CAGTTCTGATTAAAAGTCAGAATAGACANCATGATGTGGTTCCCTACATGGCACAAGGAG TGATTGGATACAANGAGAAGTTTGGGTTTGATCCTTTCATTAGCACTAACATCCCATATT TTCTGGATCGGGGTTATACCAACCGCATCTCTTTTCGCATGCTTATTAATCAGCACAGAA GTATCGATCCCACCTGTTACGTGGCGGATGTGGTGAAAGATGCTATGAAACAGCCAGATG CTGGTGAACAGTATACCTGGAAGCTCCGAACCTGGAGTTGAAGAATCAATGCAAACGGCC GAAAAACTTTAAGGTGGGTTATTTGCCCCCCACCCGTGTAAAGGCCATTTGAGTTGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005391 |
| Insert Size | 3100 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005391.1, NP_005382.1 |
| RefSeq Size | 1599 bp |
| RefSeq ORF | 1221 bp |
| Locus ID | 5165 |
| UniProt ID | Q15120 |
| Domains | HATPase_c |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] Transcript Variant: This variant (2) differs at the 3' end compared to variant 1, resulting in a shorter isoform (2) lacking the last 9 aa from the C-terminus, compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202207 | PDK3 (Myc-DDK-tagged)-Human pyruvate dehydrogenase kinase, isozyme 3 (PDK3), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 5488.00 |
|
| RC202207L1 | Lenti ORF clone of Human pyruvate dehydrogenase kinase, isozyme 3 (PDK3), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged |
CNY 7888.00 |
|
| RC202207L2 | Lenti ORF clone of Human pyruvate dehydrogenase kinase, isozyme 3 (PDK3), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RC202207L3 | Lenti ORF clone of Human pyruvate dehydrogenase kinase, isozyme 3 (PDK3), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged |
CNY 7888.00 |
|
| RC202207L4 | Lenti ORF clone of Human pyruvate dehydrogenase kinase, isozyme 3 (PDK3), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged |
CNY 7888.00 |
|
| RG202207 | PDK3 (tGFP-tagged) - Human pyruvate dehydrogenase kinase, isozyme 3 (PDK3), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 7088.00 |
|
| SC322362 | PDK3 (untagged)-Human pyruvate dehydrogenase kinase, isozyme 3 (PDK3), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 5488.00 |
