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DEPTOR (NM_022783) Human Untagged Clone
CAT＃: SC112427
DEPTOR (untagged)-Human DEP domain containing MTOR-interacting protein (DEPTOR)
CNY 5,488.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DEP.6; DEPDC6 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112427 sequence for NM_022783 edited (data generated by NextGen Sequencing)
ATGGAGGAGGGCGGCAGCACTGGCAGTGCTGGCAGTGACAGCAGCACCAGCGGGAGTGGC GGGGCGCAGCAAAGGGAGCTGGAGCGCATGGCTGAGGTCTTGGTCACCGGGGAACAGCTA CGGCTCAGGCTGCATGAAGAAAAGGTTATTAAAGATAGACGTCATCATCTCAAGACCTAC CCAAACTGTTTTGTCGCAAAAGAACTGATTGACTGGCTGATTGAACACAAAGAGGCTTCT GACAGAGAGACGGCAATTAAACTCATGCAGAAATTAGCAGACCGGGGCATTATTCACCAT GTGTGTGATGAGCATAAGGAATTCAAGGATGTCAAACTCTTCTACCGCTTTAGAAAGGAT GACGGCACCTTCCCATTGGATAATGAAGTGAAGGCCTTTATGAGAGGACAGAGGCTATAT GAAAAGCTGATGAGCCCTGAAAACACACTCCTGCAGCCCAGGGAGGAGGAAGGGGTCAAG TATGAGCGCACCTTCATGGCATCTGAATTCCTGGACTGGCTGGTTCAGGAAGGTGAGGCC ACCACGAGGAAAGAGGCAGAGCAGCTTTGCCACCGGCTTATGGAGCATGGCATCATCCAG CATGTGTCCAGCAAGCACCCATTTGTGGACAGCAATCTTCTCTACCAGTTCAGAATGAAC TTCCGGCGGAGGCGAAGACTGATGGAGCTGCTCAATGAAAAGTCCCCCTCCTCCCAGGAA ACTCATGACAGTCCCTTCTGCCTGAGGAAGCAGAGCCATGACAATCGGAAATCTACCAGC TTTATGTCAGTGAGCCCCAGCAAGGAGATCAAGATCGTGTCTGCAGTGAGGAGAAGCAGC ATGAGCAGCTGTGGCAGCAGCGGCTACTTCAGCAGCAGCCCCACCCTCAGCAGCAGCCCC CCTGTGCTCTGCAACCCCAAGTCCGTGCTGAAGAGACCTGTCACCTCTGAGGAACTCCTT ACTCCCGGGGCTCCGTATGCAAGGAAGACATTCACGATTGTTGGTGACGCGGTTGGCTGG GGTTTTGTGGTGCGAGGAAGTAAGCCATGCCACATCCAGGCTGTAGACCCCAGTGGCCCT GCAGCCGCAGCAGGAATGAAGGTCTGTCAGTTTGTCGTCTCTGTCAACGGGCTCAATGTC CTGCATGTAGACTACCGGACCGTGAACAATCTGATTCTGACGGGCCCACGGACGATTGTC ATGGAAGTCATGGAGGAGTTAGAGTGCTGA Clone variation with respect to NM_022783.2 135 c=>t;611 a=>g;1166 g=>a >OriGene 5' read for NM_022783 unedited
NGCGTCANGATTAGTAAACGACTCATATAGGGCGGCCGCGAATTCGCACCAGGCCAATCC AGTCAGAGCAGCGGAGCTGCCCCGAAAAANTGGCGCGGGAAGCGTCTGTGAGGGCAGACT GATCCGAGCACCCAAACCCTCGGCGGACAGCGGAGCCAGTGGTAGCCGCACGGCCCTAAA ACCATGGAGGAGGGCGGCAGCACTGGCAGTGCTGGCAGTGACAGCAGCACCAGCGGGAGT GGCGGGGCGCAGCAAAGGGAGCTGGAGCGCATGGCTGAGGTCTTGGTCACCGGGGAACAG CTACGGCTCAGGCTGCATGAAGAAAAGGTTATTAAAGATAGACGTCATCATCTCAAGACC TACCCAAACTGTTTTGTCGCAAAAGAACTGATTGACTGGCTGATTGAACACAAAGAGGCT TCTGACAGAGAGACGGCAATTAAACTCATGCAGAAATTAGCAGACCGGGGCATTATTCAC CATGTGTGTGATGAGCATAAGGAATTCAAGGATGTCAAACTCTTCTACCGCTTTAGAAAG GATGACGGCACCTTCCCATTGGATAATGAAGTGAAGGCCTTTATGAGAGGACAGAGGCTA TATGAAAAGCTGATGAGCCCTGAAAACACACTCCTGCAGCCCAGGNAGGGAGAAGGNGTC AAGTATGAGCGCACCTTCATGGCATCTGAATTCCTGGACTGGCTGGTTCANGAAAGTGAG GCCACCACGAGGAAAAGAGCAGAGCAGCTTTTGCACGGCTTATGGAGCATGGCATCATCC AGCATGTGTCCAGCAAGCACCCATTTGTGGACAGCAATCTTCTCTACCAGTTCAGAATGA ACTTCCGNNNCGAGCGAANACTGATGGAGCTGCTCAATGAAAAAGTCCCCTCCCTCCCAG AAA >OriGene 3' read for NM_022783 unedited
GACTACTTATGGTCCGCGCCGCATCTCTANGATCAGTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTCCTGGCCAAAGGGGGGTTTTTAAATTATGGGTTT AACCAAACTTCTTTTTTTAAACTTTCAACATTTTCGGGATTATTTCTAAAAAAAGCCTTT GCCTGTTTTATAAAACAAATGGTTTTAGGTCCAAAAAAAAATTGGGGGCCCCCATTTTCG GGGGGCCCCCCCCAAACTCCCCCCAACCATAGCTTCCCAGGGTTTTTTTTTTCCCTAAGG CCCCATTGGAACCCGGTTTAACCTTAAAGGTGGCCCCTAAGGGCGGGATTTAAAAAGCCT TTTTTTTGGGCTTTAAACCCCTTGAAGGCTTTAAACCCCCCTTGAAAATGCTAGGGAACA GGTCTAATCCCTTAAAAGGGGGCTTGGATTGGAACCTGGGGAAAACCAAATTCCCCTATA AAAAACCGGCGGGCTATTTTGGGAAAAAGCCCATTTTAAAACCTTTACCACCTCCTCTTT GGCCCCTGAAAAAGGGGACTTTACCTTGCCGCTGTTGGGCCTAAAAAACCTTTTAACCCC CTTTTTGCAACCTAAGATAAAAACCATGGATGGAAACCTCCAATTGGAATTCTGGTAGGG CAGTTCTTTCAGGTAGGAAAAGGGTTTCCCAATTGTTTTCTACCCGGACTTTTTTTAAAA AAAATATGATCCCCGGTTCTCTCCCTTTCGGGGTTTAAAAACCACACTTCAAACATGTAT GGGCGGAAAAACACCCGTTGGCCCAAAACCTTCCTTTGGCTGGAAAACCTTGGCTTTGCA TGGCTTGGGGGCATTTTGGCCTTCCCCACCCCCGGGCCCTTGGGGGGT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_022783 |
| Insert Size | 2350 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022783.1, NP_073620.1 |
| RefSeq Size | 2187 bp |
| RefSeq ORF | 1230 bp |
| Locus ID | 64798 |
| UniProt ID | Q8TB45 |
| Domains | DEP, PDZ |
| Gene Summary | Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Argininosuccinate Synthase 1-Deficiency Enhances the Cell Sensitivity to Arginine through Decreased DEPTOR Expression in Endometrial Cancer
,Ohshima, K;Nojima, S;Tahara, S;Kurashige, M;Hori, Y;Hagiwara, K;Okuzaki, D;Oki, S;Wada, N;Ikeda, JI;Kanai, Y;Morii, E;,
Sci Rep
,PubMed ID 28358054
[DEPTOR]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205770 | DEPTOR (Myc-DDK-tagged)-Human DEP domain containing MTOR-interacting protein (DEPTOR) |
CNY 5,488.00 |
|
| RC205770L1 | Lenti ORF clone of Human DEP domain containing MTOR-interacting protein (DEPTOR), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC205770L2 | Lenti ORF clone of Human DEP domain containing MTOR-interacting protein (DEPTOR), mGFP tagged |
CNY 5,890.00 |
|
| RC205770L3 | Lenti ORF clone of Human DEP domain containing MTOR-interacting protein (DEPTOR), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC205770L4 | Lenti ORF clone of Human DEP domain containing MTOR-interacting protein (DEPTOR), mGFP tagged |
CNY 7,888.00 |
|
| RG205770 | DEPTOR (tGFP-tagged) - Human DEP domain containing MTOR-interacting protein (DEPTOR) |
CNY 7,088.00 |
