GMPPA (NM_013335) Human Untagged Clone
CAT＃: SC112661
GMPPA (untagged)-Human GDP-mannose pyrophosphorylase A (GMPPA), transcript variant 1
CNY 5488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AAMR |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112661 sequence for NM_013335 edited (data generated by NextGen Sequencing)
ATGCTCAAAGCGGTGATCCTGATTGGAGGCCCTCAAAAGGGAACTCGCTTCAGACCTTTG TCTTTTGAGGTGCCCAAACCATTGTTTCCTGTGGCAGGGGTCCCTATGATCCAACACCAT ATTGAAGCCTGTGCCCAGGTCCCTGGAATGCAGGAGATTCTGCTCATTGGCTTCTACCAA CCTGATGAGCCCCTCACCCAGTTCCTAGAAGCCGCCCAGCAGGAGTTTAACCTTCCAGTC AGGTACCTGCAGGAATTTGCCCCCCTAGGCACAGGGGGTGGTCTTTACCATTTTCGAGAC CAGATCCTGGCTGGGAGCCCCGAGGCATTCTTCGTGCTCAATGCTGATGTCTGCTCCGAC TTCCCCTTGAGTGCTATGTTGGAAGCCCACCGACGCCAGCGTCACCCTTTCTTACTCCTT GGCACTACGGCTAACAGGACGCAATCCCTCAACTACGGCTGCATCGTTGAGAATCCACAG ACACACGAGGTATTGCACTATGTGGAGAAACCCAGCACATTTATCAGTGACATCATCAAC TGCGGCATCTACCTCTTTTCTCCTGAAGCCTTGAAGCCTCTTCGGGATGTCTTCCAGCGT AATCAGCAGGATGGGCAATTGGAGGACTCACCAGGCTTGTGGCCAGGGGCAGGTACCATC CGCCTAGAGCAGGATGTGTTTTCAGCCCTGGCAGGGCAGGGCCAGATATACGTGCATCTC ACTGATGGTATCTGGAGTCAGATCAAGTCCGCAGGTTCAGCCCTCTACGCCTCCCGCCTC TACCTGAGCCGATACCAGGACACTCACCCAGAACGGCTGGCCAAGCACACCCCAGGGGGC CCATGGATCCGAGGGAATGTGTACATCCACCCGACCGCCAAGGTGGCCCCCTCGGCTGTG CTGGGCCCCAACGTCTCCATCGGGAAGGGGGTGACCGTGGGTGAGGGTGTGCGGCTCCGG GAGAGCATCGTCCTCCATGGAGCCACTTTGCAGGAGCACACGTGTGTTCTGCATAGCATC GTGGGCTGGGGGAGCACCGTGGGACGCTGGGCGCGCGTGGAGGGTACCCCCAGTGACCCT AACCCCAACGATCCCCGAGCCCGCATGGACAGTGAGAGCCTCTTCAAGGACGGGAAGCTG CTGCCTGCTATCACCATCCTGGGCTGCCGAGTCCGGATCCCTGCCGAGGTGCTCATCCTG AACTCGATTGTTCTGCCACACAAGGAGCTGAGCCGAAGCTTCACCAACCAGATCATCCTC TGA Clone variation with respect to NM_013335.3 1053 c=>g >OriGene 5' read for NM_013335 unedited
CCGTTCAGAATTTGATATACGACTTTACTATAGGGCGGCCGCGAATTCGCACGAGGTGAA GGGAGCTATGTGTTTAGGTAGCAGTCACCATTATGCTCAAAGCGGTGATCCTGATTGGAG GCCCTCAAAAGGGAACTCGCTTCAGACCTTTGTCTTTTGAGGTGCCCAAACCATTGTTTC CTGTGGCAGGGGTCCCTATGATCCAACACCATATTGAAGCCTGTGCCCAGGTCCCTGGAA TGCAGGAGATTCTGCTCATTGGCTTCTACCAACCTGATGAGCCCCTCACCCAGTTCCTAG AAGCCGCCCAGCAGGAGTTTAACCTTCCAGTCAGGTACCTGCAGGAATTTGCCCCCCTAG GCACAGGGGGTGGTCTTTACCATTTTCGAGACCAGATCCTGGCTGGGAGCCCCGAGGCAT TCTTCGTGCTCAATGCTGATGTCTGCTCCGACTTCCCCTTGAGTGCTATGTTGGAAGCCC ACCGACGCCAGCGTCACCCTTTCTTACTCCTTGGCACTACGGCTAACAGGACGCAATCCC TCAACTACGGCTGCATCGTTGAGAATCCACAGACACACGAGGTATTGCACTATGTGGAGA AACCCAGCACATTTATCAGTGACATCATCAACTGCGGCATCTACCTCTTTTCTCCTGAAG CCTTTGAGCCTCTTCGGGATGTCTTCCAGCGTAATCAGCAGGATGGGCAATTGGAGGACC TCACCAGCTTGTGGCCCAGGGCAGGTACCATCCGCCTAGAGCAGGATGTGTTTTCAGCCC TGGCAGGCCAGGCCCAGATATACGTGCATCTCACTGATGGTATCCTGAAGTCAGATCAAG TCGCAAGTTCAGCCCTCTACGCCTNNCGCCTTTACCTGAGCCGATACCAGGACACTCACC CAGAACGGCTGGCCAAGCACACCCAGGGGGCCCATGAAN >OriGene 3' read for NM_013335 unedited
GGGGGGCCATGNNNGATTCTTTTNNNNNNNGGNTTTTCGTGNACCCGGACCGCATTTTAN GATCGGTTTTTTTTTTTTTTTTTTTTCCAGGTTCACGGGGTTTATTAGGGAGTCGGGAGG GAGAAAACCCAGGAGTCCCCAGGCCATCCACATTGCTCCCCGGCATGTGACGATCCAGCC TGGCTTTCTCTGGTCCTTTCTGGACAGAGGCTGGCCAAGCAGGCAGCAGCCTCAAGGGGA GTGGGTAGGAGCTGGGGGCCTTCTGGCAGCCCTACTCAGAGGATGATCTGGTTGGTGAAG CTTCGGCTCAGCTCCTTGTGTGGCAGAACAATCGAGTTCAGGATGAGCACCTCGGCAGGG ATCCGGACTCGGCAGCCCAGGATGGTGATAGCAGGCAGCAGCTTCCCGTCCTTGAAGAGG CTCTCACTGTCCATGCGGGCTCGGGGATCGTTGGGGTTAGGGTCACTGGGGGTACCCTCC ACGCGCGCCCAGCGTCCCACGGTGCTCCCCCAGCCCACGATGCTATGCAGAACACACGTG TGCTCCTGCAAAGTGGCTCCATGGAGGACGATGCTCTCCCGGAGCCGCACACCCTCACCC ACGGTCACCCCCTTCCCGATGGAGACGTTGGGGCCCAGCACAGCCGAGGGGGCCACCTTG CCGGTCGGGTGGATGTACACATTCCCCTCGATCCATGGGCCCCCTGGGGTGTGCTTGGCC AGCCGTTCTGGGTGAGTGTCCTGGTATCCGCTCAGGTAAGGCGGGAGGCCTAAAAGCCTT GACCTGCGGACTGGATCTGACTTCAGAACCCTCATGGAAATGCCCGTTTTTTGGCCCTGC CCTGCCAGGGCTGAAAACAATTCTGCTCTAGGCCGATGGACCCTGCCCTGGCCAAAACCC GGGGAAGCCCTCAATGGCCATCTGCTGATACCCCTGAAAGACATCCGAAAAGCCTTAAGG TTTCAGGAAAAAAAAGTAATCCCCCATTGATGAGTCCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_013335 |
| Insert Size | 1570 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013335.2, NP_037467.2 |
| RefSeq Size | 1543 bp |
| RefSeq ORF | 1263 bp |
| Locus ID | 29926 |
| UniProt ID | Q96IJ6 |
| Domains | hexapep, NTP_transferase |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways |
| Gene Summary | This gene is thought to encode a GDP-mannose pyrophosphorylase. This enzyme catalyzes the reaction which converts mannose-1-phosphate and GTP to GDP-mannose which is involved in the production of N-linked oligosaccharides. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) uses two different splice sites in the 5' UTR, compared to variant 2. Variants 1 and 2 encode the same protein. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200149 | GMPPA (Myc-DDK-tagged)-Human GDP-mannose pyrophosphorylase A (GMPPA), transcript variant 1 |
CNY 5488.00 |
|
| RC200149L1 | Lenti-ORF clone of GMPPA (Myc-DDK-tagged)-Human GDP-mannose pyrophosphorylase A (GMPPA), transcript variant 1 |
CNY 7888.00 |
|
| RC200149L2 | Lenti-ORF clone of GMPPA (mGFP-tagged)-Human GDP-mannose pyrophosphorylase A (GMPPA), transcript variant 1 |
CNY 5890.00 |
|
| RC200149L3 | Lenti-ORF clone of GMPPA (Myc-DDK-tagged)-Human GDP-mannose pyrophosphorylase A (GMPPA), transcript variant 1 |
CNY 5890.00 |
|
| RC200149L4 | Lenti-ORF clone of GMPPA (mGFP-tagged)-Human GDP-mannose pyrophosphorylase A (GMPPA), transcript variant 1 |
CNY 5890.00 |
|
| RG200149 | GMPPA (tGFP-tagged) - Human GDP-mannose pyrophosphorylase A (GMPPA), transcript variant 1 |
CNY 7088.00 |
|
| SC320736 | GMPPA (untagged)-Human GDP-mannose pyrophosphorylase A (GMPPA), transcript variant 1 |
CNY 5488.00 |
