EIF2B3 (NM_020365) Human Untagged Clone
CAT＃: SC113103
EIF2B3 (untagged)-Human eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa (EIF2B3), transcript variant 1
CNY 3656.00
CNY 7220.00
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EIF-2B; EIF2Bgamma |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113103 sequence for NM_020365 edited (data generated by NextGen Sequencing)
ATGGAATTTCAAGCAGTAGTGATGGCAGTAGGTGGAGGATCTCGGATGACAGACCTAACT TCCAGCATTCCCAAACCTCTGCTTCCAGTTGGGAACAAACCTTTAATTTGGTACCCATTG AACCTGCTTGAGCGTGTTGGATTTGAAGAAGTCATTGTGGTTACAACCAGGGATGTTCAA AAGGCTCTATGTGCAGAATTCAAGATGAAAATGAAGCCAGATATTGTGTGTATTCCTGAT GATGCTGACATGGGAACTGCAGATTCTTTGCGCTACATATATCCAAAACTTAAGACAGAT GTGCTGGTGCTGAGCTGTGATCTGATAACAGACGTTGCCTTACATGAGGTTGTGGACCTG TTTAGAGCTTATGATGCATCACTTGCTATGTTGATGAGAAAAGGCCAAGATAGCATAGAA CCTGTTCCCGGTCAAAAGGGGAAAAAAAAAGCAGTGGAGCAGCGTGACTTCATTGGAGTG GACAGCACAGGAAAGAGGCTGCTCTTCATGGCTAATGAAGCAGACTTGGATGAAGAGCTG GTCATTAAGGGATCCATCCTACAGAAGCATCCTAGAATACGTTTCCACACGGGTCTTGTG GATGCCCACCTCTACTGTTTGAAAAAATACATCGTGGATTTCCTAATGGAAAATGGGTCA ATAACTTCTATCCGGAGTGAACTGATTCCATATTTAGTGAGAAAACAGTTTTCCTCAGCT TCCTCACAACAGGGACAAGAAGAAAAAGAGGAGGATCTAAAGAAAAAGGAGCTGAAGTCC TTAGATATCTACAGTTTTATAAAAGAAGCCAATACACTGAACCTGGCTCCCTATGATGCC TGCTGGAATGCCTGTCGAGGAGACAGGTGGGAAGACTTGTCCAGATCACAGGTGCGCTGC TATGTCCACATCATGAAAGAGGGGCTCTGCTCTCGAGTGAGCACACTGGGACTCTACATG GAAGCAAACAGACAGGTGCCCAAATTGCTGTCTGCTCTCTGTCCAGAAGAACCACCAGTC CATTCGTCAGCCCAGATTGTCAGCAAACACCTGGTTGGAGTTGACAGCCTCATTGGGCCA GAGACACAGATTGGAGAGAAGTCATCCATTAAGCGCTCAGTCATTGGCTCATCCTGTCTC ATAAAAGATAGAGTGACTATTACCAATTGCCTTCTCATGAACTCAGTCACTGTGGAGGAA GGAAGCAATATCCAAGGCAGTGTCATCTGCAACAATGCTGTGATCGAGAAGGGTGCAGAC ATCAAGGACTGCTTGATTGGAAGTGGCCAGAGGATTGAAGCCAAAGCTAAACGAGTGAAT GAGGTGATCGTGGGGAATGACCAGCTCATGGAGATCTGA Clone variation with respect to NM_020365.3 243 c=>t >OriGene 5' read for NM_020365 unedited
CACATTTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGGCGTGCGGGG AGCTGAGTTATAGCTGTGACTTCTGCCCTGCCAGGCCGCACACAAGCTGGCTGACCCGGT TTGTAAAAATGGAATTTCAAGCAGTAGTGATGGCAGTAGGTGGAGGATCTCGGATGACAG ACCTAACTTCCAGCATTCCCAAACCTCTGCTTCCAGTTGGGAACAAACCTTTAATTTGGT ACCCATTGAACCTGCTTGAGCGTGTTGGATTTGAAGAAGTCATTGTGGTTACAACCAGGG ATGTTCAAAAGGCTCTATGTGCAGAATTCAAGATGAAAATGAAGCCAGATATTGTGTGTA TTCCTGATGATGCTGACATGGGAACTGCAGATTCTTTGCGCTACATATATCCAAAACTTA AGACAGATGTGCTGGTGCTGAGCTGTGATCTGATAACAGACGTTGCCTTACATGAGGTTG TGGACCTGTTTAGAGCTTATGATGCATCACTTGCTATGTTGATGAGAAAAGGCCAAGATA GCATAGAACCTGTTCCCGGTCAAAAGGGGAAAAAAAAAGCAGTGGAGCAGCGTGACTTCA TTGGAGTGGACAGCACAGGAAAGAGGCTGCTCTTCATGGCTAATGAAGCAGACTTGGATG AAGAGCTGGTCATTAAGGGATCCATCCTACAGAAGCATCCTAGAATACGTTTCCACACGG GNTCTTGTGGATGCCCACCTCTACTGTTNGAAAAATACATCGTGGATTTCCTAATGGAAA ATGGGNNTCATACTTCTATCCNNGAGTGACTGNATNNCATATTAGTGANGAAACAGTTTT TCTCAGCTTCCCCTCACACAGGGACCAGAAGAAAAGAGNAGGATCTAANGAAAAGGAGNN CTGAGTCNTAGTATCTACANTTTTATAAAGAAGCCATACACTGAACTGGNCTCCTATGAT GCTGCTGNAATGCTGTCNAGGAAC >OriGene 3' read for NM_020365 unedited
GATCGCTGCCGCAATTTANATCTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTACCCCTGGGACCCCACCTGACATGGGACCTTTGGACTGGTC CACAAGTCTCCAGCATGCCTTTGGAAGCCCTTTTTTATTGGGAAATAAATACCGAGTTAA ACAGGGGGGCCGGCCAACATCTGGGGCTTTTTAGGCCAAAAGGAAGGAGTCTGACTTGGT CAAAACTCAAATCTCCATGAGCTGGTCATTCCCCACGATCACCTCATTCACTCGTTTAGC TTTGGCTTCAATCCTTTGGCCACTTCCAATCAAGCAGTCCTTGATGTCTGCCCCCTTTTC GATCACAGCATTGTTGCAAATGACACTGCCTTGGATATTGCTTCCTTCCTCCCAGGGACT GAGTTCATGAAAAGGCAATTGGGAATAGTCACTCTATCTTTTATGAAACAGGATGAGCCA ATGACTGAGCGCTTAATGGATGACTTCTCTCCAATCTGTGTCTCTGGCCCAATGAGGCTG TCAACTCCAACCAGGTGTTTGCTGACAATCTGGGCTGACCAATGGACTGGTGGTTCTTCT GGACAGAAAGCACACAGCAATTTGGGCACCTGTCTGTTTTGCTCCATGTAGAGTCCCCAG TGGGCTCACTCGAAAGCAGAGCCCCTTTTCATGATGTGGACATAGCAGCGCACCTGTGAT CTGGACAGATTTTCCCACCTGTTTTCTCGACAGGGATTCCAGCAGGCATTATAAGGAGCC AGGTTCCAGGGATTGCCTTCTTTTTAAAACTGGGAAATCTTAGGACTCCGCTCCTTTTTC TTTAAACCTCCCCTTTTTTTTTTGCCCCCTGTGGGAGAAACTTGAGAAACTGTTTTCCCC TAAATATGGAACAGTTCCTCCGGAAGAAATTTTGCCCCTTTCCCTTAGAAACCCCCAGTT TTTTTCAACAGAGAGGGGCCCTN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_020365 |
| Insert Size | 2000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020365.1, NP_065098.1 |
| RefSeq Size | 1602 bp |
| RefSeq ORF | 1359 bp |
| Locus ID | 8891 |
| UniProt ID | Q9NR50 |
| Domains | NTP_transferase |
| Gene Summary | The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223570 | EIF2B3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa (EIF2B3), transcript variant 1 |
CNY 3990.00 |
|
| RC223570L3 | Lenti-ORF clone of EIF2B3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa (EIF2B3), transcript variant 1 |
CNY 5890.00 |
|
| RC223570L4 | Lenti-ORF clone of EIF2B3 (mGFP-tagged)-Human eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa (EIF2B3), transcript variant 1 |
CNY 5890.00 |
|
| RG223570 | EIF2B3 (tGFP-tagged) - Human eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa (EIF2B3), transcript variant 1 |
CNY 4370.00 |
