POLR1B (NM_019014) Human Untagged Clone

Name: POLR1B (NM_019014) Human Untagged Clone
SKU: SC113295
Price: 7912.00

CAT＃: SC113295

POLR1B (untagged)-Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1

Datasheet

SDS

"NM_019014" in other vectors (6)

CNY 7,912.00

货期*

现货

规格

Product images

经常一起买 (4)

TA346803

Rabbit Polyclonal Anti-POLR1B Antibody

CNY 5,200.00

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	A135; RPA2; RPA135; Rpo1-2; TCS4
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC113295 sequence for NM_019014 edited (data generated by NextGen Sequencing) ATGGATCCTGGCAGCCGGTGGCGGAACCTGCCCAGCGGGCCTAGCCTAAAGCACTTGACT GACCCCTCTTATGGAATCCCGCGGGAACAGCAAAAGGCAGCGTTGCAGGAGCTGACGCGG GCGCACGTGGAGTCCTTCAACTACGCTGTGCACGAGGGTCTCGGCCTCGCGGTGCAGGCT ATACCTCCCTTTGAATTTGCTTTCAAAGATGAGCGTATCTCTTTTACTATTCTGGATGCT GTTATCAGTCCACCTACAGTTCCAAAAGGGACCATCTGCAAAGAGGCCAATGTTTATCCA GCAGAATGCCGGGGCCGAAGGAGTACCTACCGTGGGAAGTTGACAGCTGATATCAACTGG GCAGTGAATGGAATCTCAAAAGGAATCATTAAGCAGTTTCTTGGCTATGTTCCCATCATG GTGAAATCCAAGCTTTGCAACTTACGTAACCTTCCCCCACAAGCCCTCATTGAGCACCAT GAGGAGGCAGAGGAAATGGGGGGCTATTTTATAATCAATGGCATTGAAAAAGTCATCCGA ATGTTGATTATGCCTCGGAGAAATTTTCCCATTGCAATGATAAGACCAAAATGGAAAACC AGAGGGCCTGGTTATACTCAGTATGGAGTTTCAATGCACTGTGTGAGGGAAGAACATTCC GCTGTCAATATGAACCTCCACTACTTGGAAAATGGCACTGTTATGTTGAACTTTATTTAC CGAAAAGAACTGTTCTTTCTTCCTTTGGGATTTGCACTTAAGGCACTTGTCAGCTTTTCT GATTATCAGATCTTTCAGGAGCTCATCAAAGGAAAAGAGGATGATTCTTTCCTTAGGAAC TCTGTTTCTCAGATGTTAAGGATTGTAATGGAAGAGGGTTGTTCGACACAAAAACAGGTC CTTAACTACCTAGGTGAATGCTTCAGAGTAAAACTCAATGTTCCTGACTGGTACCCAAAT GAGCAAGCTGCGGAGTTCCTGTTTAACCAGTGCATCTGTATCCACTTGAAATCCAATACT GAAAAGTTTTATATGCTTTGTCTCATGACGCGAAAGCTCTTTGCTTTAGCCAAAGGAGAG TGCATGGAGGACAATCCTGATAGTTTGGTGAACCAGGAAGTCCTCACACCGGGTCAGCTC TTCCTTATGTTCCTGAAGGAAAAACTGGAAGGTTGGTTAGTGTCTATTAAAATAGCTTTT GATAAGAAGGCTCAGAAGACCAGTGTTTCCATGAACACTGACAATTTGATGAGGATTTTT ACAATGGGCATAGACCTTACAAAACCATTTGAATACCTTTTTGCTACTGGGAATCTGCGT TCTAAAACAGGTCTTGGCCTCCTACAAGATTCTGGACTTTGTGTTGTGGCTGACAAGCTG AACTTCATACGCTACCTCTCCCATTTCCGCTGCGTGCACAGAGGGGCTGATTTTGCCAAG ATGAGGACCACCACAGTACGCAGGCTGCTGCCAGAGTCCTGGGGCTTCCTTTGTCCCGTG CATACCCCAGACGGGGAGCCCTGTGGCCTGATGAACCACCTAACTGCCGTATGTGAGGTT GTCACACAGTTTGTGTATACGGCATCTATTCCAGCTTTACTGTGCAACTTGGGGGTCACT CCCATTGATGGAGCTCCCCACCGATCATACAGTGAGTGCTACCCTGTCCTGCTGGACGGT GTCATGGTTGGCTGGGTGGATAAGGATCTTGCTCCAGGCATCGCAGATTCTCTTCGTCAT TTTAAGGTGTTGAGAGAGAAAAGAATTCCTCCCTGGATGGAAGTGGTCCTTATACCCATG ACAGGAAAACCAAGTCTGTACCCAGGATTGTTCCTTTTTACCACTCCTTGTAGACTGGTA CGGCCTGTGCAGAACTTAGCATTGGGCAAAGAAGAGCTAATTGGAACTATGGAACAGATC TTCATGAATGTCGCTATCTTTGAGGATGAAGTTTTTGCTGGAGTTACCACACACCAGGAA CTCTTTCCACACAGCCTGCTGAGTGTGATTGCCAACTTCATCCCTTTCTCTGATCACAAC CAGAGTCCACGGAACATGTACCAATGCCAGATGGGTAAGCAAACTATGGGCTTTCCACTT CTCACTTATCAAGACCGATCGGATAACAAACTGTATCGTCTTCAGACTCCTCAGAGTCCC TTGGTGAGACCCTCCATGTATGATTATTATGACATGGATAACTATCCAATTGGGACCAAT GCCATCGTTGCTGTGATTTCTTACACTGGCTATGATATGGAAGATGCCATGATTGTGAAT AAGGCCTCTTGGGAACGAGGCTTTGCCCATGGAAGTGTCTACAAGTCTGAGTTCATAGAC CTCTCTGAAAAAATTAAACAAGGAGATAGTAGCCTGGTGTTTGGCATCAAACCTGGTGAC CCACGCGTTCTGCAGAAGTTAGATGACGATGGATTGCCGTTTATAGGAGCAAAACTGCAG TACGGAGATCCGTATTACAGCTACCTCAACCTCAACACCGGGGAAAGTTTTGTGATGTAC TATAAGAGTAAAGAAAATTGTGTTGTGGATAACATCAAAGTGTGCAGTAATGACACTGGG AGTGGAAAATTCAAGTGTGTTTGCATCACTATGAGAGTGCCTCGGAACCCAACTATCGGA GATAAATTTGCCAGTCGCCATGGGCAGAAGGGCATTTTAAGCAGATTGTGGCCGGCTGAG GACATGCCTTTTACTGAGAGTGGGATGGTCCCAGACATTCTGTTCAATCCCCATGGTTTT CCATCCCGCATGACCATTGGGATGTTAATTGAGAGTATGGCCGGGAAGTCTGCAGCTTTG CATGGTCTCTGCCATGATGCTACACCCTTCATCTTCTCAGAGGAGAACTCGGCCTTAGAA TACTTTGGTGAGATGTTAAAGGCTGCTGGCTACAATTTCTATGGCACCGAGAGGTTATAT AGTGGCATCAGTGGGCTAGAACTGGAAGCAGACATCTTCATAGGAGTGGTTTATTATCAG CGCTTACGCCATATGGTCTCAGACAAATTTCAAGTAAGGACAACTGGAGCCCGAGACAGA GTCACCAACCAGCCTATTGGGGGAAGAAATGTCCAGGGTGGAATCCGTTTTGGGGAGATG GAACGGGATGCGCTTTTAGCTCATGGTACATCTTTTCTCCTTCATGACCGCCTCTTCAAC TGCTCAGATCGGTCGGTAGCCCATGTGTGTGTGAAGTGTGGCAGTTTACTCTCTCCACTG TTGGAGAAGCCACCCCCTTCTTGGTCTGCCATGCGCAACAGAAAATACAACTGTACTCTG TGTAGTCGCAGTGACACTATCGATACTGTTTCTGTGCCTTATGTTTTTCGGTATTTTGTA GCTGAACTGGCAGCTATGAACATCAAAGTGAAACTGGATGTTGTTTAA Clone variation with respect to NM_019014.4 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_019014 unedited GGCTTCGGATTTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACCAGGGCGTCCG GTGTGCAGGTGGCCACATGGATCCTGGCAGCCGGTGGCGGAACCTGCCCAGCGGGCCTAG CCTAAAGCACTTGACTGACCCCTCTTATGGAATCCCGCGGGAACAGCAAAAGGCAGCGTT GCAGGAGCTGACGCGGGCGCACGTGGAGTCCTTCAACTACGCTGTGCACGAGGGTCTCGG CCTCGCGGTGCAGGCTATACCTCCCTTTGAATTTGCTTTCAAAGATGAGCGTATCTCTTT TACTATTCTGGATGCTGTTATCAGTCCACCTACAGTTCCAAAAGGGACCATCTGCAAAGA GGCCAATGTTTATCCAGCAGAATGCCGGGGCCGAAGGAGTACCTACCGTGGGAAGTTGAC AGCTGATATCAACTGGGCAGTGAATGGAATCTCAAAAGGAATCATTAAGCAGTTTCTTGG CTATGTTCCCATCATGGTGAAATCCAAGCTTTGCAACTTACGTAACCTTCCCCCACAAGC CCTCATTGAGCACCATGAGGAGGCAGAGGAAATGGGGGGCTATTTTATAATCAATGGCAT TGAAAAAGTCATCCGAATGTTGATTATGCCTCGGAGAAATTTTCCCATTGCAATGATAAG ACCAAAATGGAAAACCAGAGGGCCTGGTTATACTCAGTATGGAGTTTCAATGCACTGTGT GAGGGAAGACATTCCGCTGTCAATATGAACCTCCACTACTTGGAANATGGCACTGGTTAT GTTGGAACTTTATTTANCCGANAGAACTGGTCTTTCTTTCTTTGGGATTTGCACTTAAGG CACTTGTCAGCTNTTNCTGATATCAGATCTTTCAGGAGCTCATCAAGGAAAAGAGGATGA TTCTTTCCTTAGAACTCTGTTCTC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_019014 unedited GACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATCATCAAAAAACATC TTATAATTGCAGAAGTATATACAAGATGATCTATTATCAACAGTTTATAATGGCCAAAGA TAAGTCACCAGAATATTTGCAGAAAAAAGTTATAATGGCCAAAGAATGGAACAACTTAAA TACACAGGGGATGGTAAATCCATACAATAAAATACTGTACACAAGAAAGAAAACATAAGT GGCACTCTACACACTGCTATGAAAGCAGATCAATATAAAAGCAGAAAAAAGCCAAGTATG GAAGTGTAGACTAAATATAAAATACTCACCGGAGGCCAGGCACAGTGGCTCACACCCGTA ATCCTAGCACTTTCGGAGGCCGAGGTGGGTGGATCACTTGAGGTCAGGGTCTTCAAGACA GGGTCTTCCTATATTACCCAGGCTGTCCTTGAACTCCTGCACTCAAATGATCCTCCCACC TCAGCCTCTCAAATAGCAACGACTATAGATGCACAGCACCATGCCTGGCCTCCAAAATGG ATGAATCTTATTTCACTATGCTAAGTGAAATAAGCCAGTCACAGGACAAATATTGCATGA TCCCACTATATGAGATATCTGTAATAGTCAAACTCATACAAACAGGGTAACAAGGGTGGT TGCTAAGGCCCAGGGAAATGGACAGTTGTCCCACACGTATTAAGCTCTAGTTTGCCACAA CAAGCAGGTCTAAACATCTGTTGCCCACACTGGTTGCCTGCAATTAACCCCCTTATATCG CGCCTTTAAAAATTCCGTAAAAGGGTACGCTTCTTTTTTAACTCGCCCTTACCCCCCAAT AACTCCCATAAATCCTACAGCCCCTTTATTCATACAAAAATCGCGCCGCCCCCACTCTGC GCCCATCAAAGGTCTGTTATCTCCTCCTTTCCGCGGTGATGGTATCAAAGCGCCCGCCCT TTTCATCACCCCCCTATGTCTCTCTCCCCTTTATCAACTTCTATCTTCTCCCCCG
Restriction Sites	NotI-NotI
ACCN	NM_019014
Insert Size	5000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019014.3, NP_061887.2
RefSeq Size	5968 bp
RefSeq ORF	3408 bp
Locus ID	84172
UniProt ID	Q9H9Y6
Domains	RNA_pol_Rpb2_3, RNA_pol_Rpb2_5
Protein Families	Transcription Factors
Protein Pathways	Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
Gene Summary	Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC220367	POLR1B (Myc-DDK-tagged)-Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,904.00
RC220367L1	Lenti ORF clone of Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 13,210.00
RC220367L2	Lenti ORF clone of Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 13,210.00
RC220367L3	Lenti ORF clone of Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 13,210.00
RC220367L4	Lenti ORF clone of Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 13,210.00
RG220367	POLR1B (tGFP-tagged) - Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,504.00

其它POLR1B产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies