VPS33B (NM_018668) Human Untagged Clone
CAT＃: SC113391
VPS33B (untagged)-Human vacuolar protein sorting 33 homolog B (yeast) (VPS33B)
CNY 4600.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113391 sequence for NM_018668 edited (data generated by NextGen Sequencing)
ATGGCTTTTCCCCATCGGCCGGACGCCCCTGAGCTGCCTGACTTCTCCATGCTGAAGAGG CTGGCTCGAGACCAGCTCATCTATCTGCTGGAGCAGCTTCCTGGAAAAAAGGATTTATTC ATTGAGGCAGATCTCATGAGCCCTTTGGATCGAATTGCCAATGTCTCCATCCTGAAGCAA CACGAAGTAGACAAGCTATACAAGGTGGAGAACAAGCCAGCCCTCAGCTCCAATGAACAA TTGTGCTTCTTGGTCAGACCCCGCATCAAGAATATGCGATACATTGCCAGTCTTGTCAAT GCTGACAAATTGGCTGGCCGAACTCGCAAATACAAAGTGATCTTCAGCCCTCAAAAGTTC TATGCGTGTGAGATGGTGCTTGAGGAAGAGGGAATCTATGGAGATGTGAGCTGTGATGAA TGGGCCTTCTCTTTGCTGCCTCTTGATGTGGATCTGCTGAGCATGGAACTACCAGAATTT TTCAGGGATTACTTTCTGGAAGGAGATCAGCGTTGGATCAACACTGTAGCTCAGGCCTTA CACCTTCTCAGCACTCTCTATGGACCCTTTCCAAACTGCTATGGAATTGGCAGGTGCGCC AAGATGGCATATGAATTGTGGAGGAACCTGGAGGAGGAGGAGGATGGCGAAACCAAGGGC CGAAGGCCAGAGATTGGACATATCTTTCTCTTGGACAGAGATGTGGACTTTGTGACAGCA CTTTGCTCCCAAGTGGTTTATGAGGGCCTAGTAGATGACACCTTCCGCATCAAGTGTGGG AGTGTCGACTTTGGCCCAGAAGTCACATCCTCTGACAAGAGCCTGAAGGTGCTACTCAAT GCCGAGGACAAGGTGTTTAATGAGATTCGGAACGAGCACTTCTCCAATGTCTTTGGCTTC TTGAGCCAGAAGGCCCGGAACTTGCAGGCCCAGTATGATCGCCGGAGAGGCATGGACATT AAGCAGATGAAGAATTTCGTGTCCCAGGAGCTCAAGGGCCTGAAACAGGAGCACCGCCTG CTGAGTCTCCATATTGGGGCCTGTGAATCCATCATGAAGAAGAAAACCAAGCAGGATTTC CAGGAGCTAATCAAGACTGAGCATGCACTGCTAGAGGGGTTCAACATCCGGGAGAGCACC AGCTACATTGAGGAACACATAGACCGGCAGGTGTCGCCTATAGAAAGCCTGCGCCTCATG TGCCTTTTGTCCATCACTGAGAATGGTTTGATCCCCAAGGATTACCGATCTCTGAAAACA CAGTATCTGCAGAGCTATGGCCCTGAGCACCTGCTAACCTTCTCCAATCTGCGAAGAGCT GGGCTCCTAACGGAGCAGGCCCCCGGGGACACCCTCACAGCCGTGGAGAGTAAAGTGAGC AAGCTGGTGACCGACAAGGCTGCAGGAAAGATTACTGATGCCTTCAGTTCTCTGGCCAAG AGGAGCAATTTTCGTGCCATCAGCAAAAAGCTGAATTTGATCCCACGTGTGGACGGCGAG TATGATCTGAAAGTGCCCCGAGACATGGCTTACGTCTTCGGTGGTGCTTATGTGCCCCTG AGCTGCCGAATCATTGAGCAGGTGCTAGAGCGGCGAAGCTGGCAGGGCCTTGATGAGGTG GTACGGCTGCTCAACTGCAGTGACTTTGCATTCACAGATATGACTAAGGAAGACAAGGCT TCCAGTGAGTCCCTGCGCCTCATCTTGGTGGTGTTCTTGGGTGGTTGTACATTCTCTGAG ATCTCAGCCCTCCGGTTCCTGGGCAGAGAGAAAGGCTACAGGTTCATTTTCCTGACGACA GCAGTCACAAACAGCGCTCGCCTTATGGAGGCCATGAGTGAGGTGAAAGCCTGA Clone variation with respect to NM_018668.3 >OriGene 5' read for NM_018668 unedited
GTCGAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTGAGACAGGC TGCTCCGAGGTAGTAGCTCTCTTGCCTGGAGGTGGCCATTCATTCCTGGAGTGCTGCTGA GGAGCGAGGGCCCATCTGGGGTCTCTGGAAGTCGGTGCCCAGGCCTGAAGGATAGCCCCC CTTGCGCTTCCCTGGGCTGCGGCCGGCCTTCTCAGAACGAAGGGCGTCCTTCCACCCCGC GGCGCAGGTGACCGCTGCCATGGCTTTTCCCCATCGGCCGGACGCCCCTGAGCTGCCTGA CTTCTCCATGCTGAAGAGGCTGGCTCGAGACCAGCTCATCTATCTGCTGGAGCAGCTTCC TGGAAAAAAGGATTTATTCATTGAGGCAGATCTCATGAGCCCTTTGGATCGAATTGCCAA TGTCTCCATCCTGAAGCAACACGAAGTAGACAAGCTATACAAGGTGGAGAACAAGCCAGC CCTCAGCTCCAATGAACAATTGTGCTTCTTGGTCAGACCCCGCATCAAGAATATGCGATA CATTGCCAGTCTTGTCAATGCTGACAAATTGGCTGGCCGAACTCGCAAATACAAAGTGAT CTTCAGCCCTCAAAAGTTCTATGCGTGTGAGATGGTGCTTGAGGAAGAGGGAATCTATGG AGATGTGAGCTGTGATGAATGGGCCTTCTCTTTGCTGCCTCTTGATGTGGATCTGCTGAG CATGGAACTACCAGAATTTTTCAGGGATTACTTTCTGGAAGGAGATCAGCGTTGGATCAA CACTGTAGCTCAAGCCTTACACCTTCTCAGCACTCTCTATGGACCCTNTCCAACTGCTAT GGAATTGGCAGGTGCGCCAAGATGGCATATGAAANTGTGGAGGAACCTGNNNAGAGNAGG AGGATGGCGAAACCCAGGGCCGAAGCCAAA >OriGene 3' read for NM_018668 unedited
ACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTCTGAAAGCAAAGGCTTTATTTACAATGACATTCAAACAGGATTTAA CAAAGGATGCCTTTTCCTGCTCGCATTTTAACAGCATGGGTTGTACTTCATAAACAAAAA AAAAAAATTTCCTGGGAGCAGGAAGGGAACTCTTTTCTCAAATAATGTTCTTTAAATCCC AACACGTTCCATGCTCCCGGTTTTTAACAGGTAGTTGGGGGACACTTGGTTATAACAGCT GGGTGCCAAATGCCTGCATCTCACTGAGGAATGGGTTCAGGGAAAATGTCAACACTGGCC GGGAAAAACATCAGGCTTTCACCTCACTCATGGCCTCCATAAGGCGAGCCCTGTTTGTGA CTGCTGTCGTCAGGAAAATGAACCTGTAGCCTTTTTTTTTGCCCAGGAACCGGAGGGCTG AAATCTCAAAAAATGTCCAACCACCCAAAAACACCACCAAGATGAGGCGCAGGGACTCAC TGGAAGCCTTGTCTTCCTTAGTCATATCTGGGAATGCAAAGTCACTGCAGTTGAGCAGCC GTACCACCTCATTAAGGCCCTGCCAGCTTCGCCGCTCTAACACCTGCTTAATGATTCGGC AGCTCAGGGGCACATAAGCCCCACCGAAGACGTAAGCCCTGTCTCGGGGCACTTTCAGAT ATACTCGCCCGGCCACACGTGGGGATCAAATCCACTTTTTGCTGATGGACCAAAATTGCT CCTTTTTGCCACAAAACTGGAAGCATCATTAATCTTTCCTGGCATCTTGGTCGTCACCAG CTTGTTCACTTTACTTTCCAGCTTTGAAAGGTGTCCCCGGGGCCCTGTCCCTAGGAACCC CTCTTTTCCAAATGGAAAAGTTACCAGTGCTCACGGCCT |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_018668 |
| Insert Size | 2380 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018668.2, NP_061138.2 |
| RefSeq Size | 2509 bp |
| RefSeq ORF | 1854 bp |
| Locus ID | 26276 |
| UniProt ID | Q9H267 |
| Domains | Sec1 |
| Gene Summary | Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and encodes the human ortholog of rat Vps33b which is homologous to the yeast class C Vps33 protein. The mammalian class C vacuolar protein sorting proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Mutations in this gene are associated with arthrogryposis-renal dysfunction-cholestasis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203870 | VPS33B (Myc-DDK-tagged)-Human vacuolar protein sorting 33 homolog B (yeast) (VPS33B) |
CNY 6888.00 |
|
| RC203870L3 | Lenti ORF clone of Human vacuolar protein sorting 33 homolog B (yeast) (VPS33B), Myc-DDK-tagged |
CNY 6460.00 |
|
| RC203870L4 | Lenti ORF clone of Human vacuolar protein sorting 33 homolog B (yeast) (VPS33B), mGFP tagged |
CNY 6460.00 |
|
| RG203870 | VPS33B (tGFP-tagged) - Human vacuolar protein sorting 33 homolog B (yeast) (VPS33B) |
CNY 8488.00 |
|
| SC323727 | VPS33B (untagged)-Human vacuolar protein sorting 33 homolog B (yeast) (VPS33B) |
CNY 4600.00 |
