POLR3E (NM_018119) Human Untagged Clone
CAT＃: SC113682
POLR3E (untagged)-Human polymerase (RNA) III (DNA directed) polypeptide E (80kD) (POLR3E)
CNY 5192.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C37; RPC5; SIN |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113682 sequence for NM_018119 edited (data generated by NextGen Sequencing)
ATGGCCAATGAAGAGGATGACCCAGTTGTACAGGAGATCGATGTGTACTTGGCCAAGAGT CTGGCGGAAAAGCTGTATCTATTTCAGTACCCTGTGCGTCCAGCCTCGATGACCTACGAT GACATTCCGCACCTCTCAGCCAAGATCAAGCCCAAGCAGCAGAAGGTAGAGCTTGAGATG GCCATCGACACCCTGAACCCCAACTATTGCCGCAGCAAAGGGGAGCAGATTGCGCTGAAC GTGGACGGGGCCTGCGCCGACGAGACCAGCACGTATTCCTCGAAGCTGATGGACAAGCAG ACCTTCTGCTCTTCCCAGACCACCAGTAACACATCCCGTTATGCCGCTGCACTCTACAGG CAAGGTGAGCTCCACCTGACACCTTTACATGGCATCCTGCAGCTGCGGCCCAGCTTCTCC TACCTGGATAAGGCTGACGCCAAGCACCGGGAGAGGGAGGCGGCCAACGAGGCAGGGGAC TCTTCACAGGATGAGGCGGAAGACGATGTTAAGCAGATCACGGTGCGGTTCTCCCGGCCG GAGTCAGAGCAGGCCCGCCAGCGCCGTGTGCAGTCCTATGAGTTCCTGCAGAAGAAGCAC GCAGAGGAGCCCTGGGTCCACCTGCATTACTATGGCCTGAGGGACAGTCGCTCTGAGCAT GAGCGTCAGTACCTGCTGTGCCCCGGCTCAAGCGGGGTGGAGAACACGGAGCTCGTCAAG TCACCCAGTGAGTACCTGATGATGCTGATGCCACCCAGCCAGGAGGAGGAGAAAGACAAG CCTGTGGCCCCCAGCAACGTCCTGTCGATGGCCCAGCTGCGCACGCTGCCCCTGGCCGAT CAGATCAAGATCCTGATGAAGAATGTGAAGGTCATGCCTTTTGCCAACTTGATGAGCCTC CTGGGCCCCTCCATCGATTCCGTGGCTGTTCTGCGGGGCATCCAGAAGGTGGCGATGTTG GTCCAAGGGAACTGGGTGGTGAAGAGTGACATCCTATACCCCAAGGACTCGTCCAGCCCT CACAGCGGCGTGCCTGCTGAGGTGCTCTGCAGGGGCCGAGACTTCGTTATGTGGAAGTTC ACGCAGAGCCGCTGGGTGGTTAGGAAAGAGGTGGCAACCGTGACCAAACTCTGCGCCGAG GATGTGAAGGACTTCCTGGAGCACATGGCCGTGGTGAGGATCAACAAAGGCTGGGAGTTC ATTCTGCCTTATGATGGGGAGTTCATCAAGAAGCACCCGGATGTGGTCCAGCGGCAGCAC ATGCTGTGGACGGGTATCCAGGCCAAACTGGAAAAAGTCTATAATCTTGTAAAGGAAACC ATGCCAAAGAAGCCGGATGCACAATCAGGGCCTGCCGGGCTGGTCTGTGGGGACCAGCGG ATCCAAGTAGCCAAAACCAAGGCCCAGCAGAACCACGCGTTGCTGGAGCGGGAGCTGCAG CGGCGGAAGGAGCAGCTGCGGGTGCCTGCGGTCCCGCCCGGTGTGCGGATCAAGGAGGAG CCCGTGAGCGAGGAGGGCGAGGAGGACGAGGAGCAGGAGGCGGAGGAGGAGCCCATGGAC ACTTCCCCCAGCGGCCTCCACAGCAAGCTGGCCAACGGGCTGCCTCTCGGGCGGGCTGCG GGCACAGACAGCTTCAACGGGCACCCGCCCCAGGGCTGCGCCAGCACCCCTGTGGCTCGG GAACTGAAGGCCTTCGTGGAGGCCACCTTTCAGAGACAGTTTGTGCTCACGCTGAGCGAA CTCAAGCGCCTCTTCAATCTGCACTTGGCCAGCCTGCCCCCCGGCCACACACTCTTCAGC GGCATCTCGGACCGCATGCTACAGGACACGGTGCTGGCCGCCGGTTGCAAGCAGATACTG GTGCCTTTTCCCCCCCAGACTGCTGCTTCCCCGGATGAGCAGAAGGTGTTTGCCCTCTGG GAGTCTGGAGACATGAGTGATCAGCATCGACAGGTTTTGCTTGAAATTTTTTCCAAAAAT TACCGGGTACGCCGAAACATGATCCAGTCTCGGTTGACTCAAGAGTGTGGAGAAGATCTC AGTAAACAGGAGGTGGATAAAGTACTAAAGGACTGCTGTGTAAGCTATGGTGGCATGTGG TACCTTAAAGGGACAGTACAGTCTTGA Clone variation with respect to NM_018119.2 >OriGene 5' read for NM_018119 unedited
CATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGCTGAGAGGAGA GCCGGGCCGCCGCCGTCTCTGCAGCCCGCGGGTAACTGGGCCGTTGCCGCCGTCCGCGCT CGGCCCCCGCGGAGAGATCGAGCTGAAGGACTGCGCGGCTGGCTCTCCTCTAGTATGGCC AATGAAGAGGATGACCCAGTTGTACAGGAGATCGATGTGTACTTGGCCAAGAGTCTGGCG GAAAAGCTGTATCTATTTCAGTACCCTGTGCGTCCAGCCTCGATGACCTACGATGACATT CCGCACCTCTCAGCCAAGATCAAGCCCAAGCAGCAGAAGGTAGAGCTTGAGATGGCCATC GACACCCTGAACCCCAACTATTGCCGCAGCAAAGGGGAGCAGATTGCGCTGAACGTGGAC GGGGCCTGCGCCGACGAGACCAGCACGTATTCCTCGAAGCTGATGGACAAGCAGACCTTC TGCTCTTCCCAGACCACCAGTAACACATCCCGTTATGCCGCTGCACTCTACAGGCAAGGT GAGCTCCACCTGACACCTTTACATGGCATCCTGCAGCTGCGGCCCAGCTTCTCCTACCTG GATAAGGCTGACGCCAAGCACCGGGAGAGGGAGGCGGCCAACGAGGCAGGGGACTCTTCC CAGGATGAGGCGGAAGACGATGTTAAGCAGATCACGGCGCGGTTCTCCCGGCCGGAGTCA GAGCAAGCCCGCCAGCGCCGTGTGCAGTCCTATGAGTCCCTGCCAAAGAAGCACCCACAG GAGCCCCTGGTCCACCTGCATTACTATGGCCCTGAGGGACAGTCGCTCTGAGCATGAGCG TCAGTACCTGCTGTGCCCCGCTCCAGCGGGCTGGAGAACACGAACTCGTAAGTTCCCCAT GAGACCTGTTGATGCTGATGCCCCCAGCCAGAAGTAGAGCAGGACAGCCCGTGCCCC >OriGene 3' read for NM_018119 unedited
CCGCAATCTAAAGTCGAGTNTTTTTTTTTTTTTTTTTTTTTAGCTAAGAGTTTGCATTTA TTTTAAAAATACACAAAATAGAAATTTTTACATCAAAGTGTGATAACCTCACTTACACAT TGTTCCATACTTACCTGGTTTTGTTTGCATCTTTCTGCAAACATTAAAAGGAGATGGATT TGATTCTGATTTTTTTGCTATGGTTCATGTAAACAGTTGAGACTGCTACATAAAGTAAGT TGTTGTCAAAGGTGAAGTGGCCACAGAATCCCAAGAATAGAATAATTCAATTTGGTTTAA TGAAATTGGTGGAGGTCTTATCAGATAGATAATCCAAGACTAAATATTGTCTTCTAAGCA TTTTAAAAATTAAGAACTTTGAAGTTTTCTTCATGTTGTAAACATAACTTAGACCTTGTT GGCATTAAGTTTACAAAAGAAAATATTAAACCATGATTTTTATCATCCTGCCCATGTCAG TATACACTCTCTTTATTATGAGAATGAAACCAAATAATAAGCAAAATACATCAGGAATTT CAAATTGTACTGCAAAGAAAGTCCCAGCTGGTCTCTTCTGGGAGTGATCTAACTAACTTA AGCTGACCCTGTGACTGGCTGAAGATAATCCCTTCTGTCCACTGCACCGTGCAATGCCAC AGGTCATGAGATGGTCAGTTCCTCTTGCTCTGTGTCGTCTGAAGCAGTCGAGGCCCTACT TCTGTTCCGNCCTTCTTCTTGGGCTANATTTGCTGGGTTAGTAGTTGCTCTATTGTCAGA CTGACTGTCCCTTTAAGTACACATGCCACCTAGCTACCAGCAGNCCTTATACTTATCCAC CTCTGTTACTGGATCTCTCCACCTCTTGAGTCACCGAACTGATCATGTTTGGGTCCCCGA ATTNTGGAAAATTCAAGCAAACTGCAAGCTATACTCTGTTCCGACTCCAAGGAAAACCTT GTCATCGGGATG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018119 |
| Insert Size | 3270 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018119.2, NP_060589.1 |
| RefSeq Size | 2640 bp |
| RefSeq ORF | 2127 bp |
| Locus ID | 55718 |
| UniProt ID | Q9NVU0 |
| Domains | Sin_N |
| Protein Families | Transcription Factors |
| Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218588 | POLR3E (Myc-DDK-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide E (80kD) (POLR3E) |
CNY 5184.00 |
|
| RC218588L3 | Lenti ORF clone of Human polymerase (RNA) III (DNA directed) polypeptide E (80kD) (POLR3E), Myc-DDK-tagged |
CNY 7700.00 |
|
| RC218588L4 | Lenti ORF clone of Human polymerase (RNA) III (DNA directed) polypeptide E (80kD) (POLR3E), mGFP tagged |
CNY 7700.00 |
|
| RG218588 | POLR3E (tGFP-tagged) - Human polymerase (RNA) III (DNA directed) polypeptide E (80kD) (POLR3E) |
CNY 6370.00 |
