PRO0456 (NM_014127) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_014127 edited GAATTCGGCACGAGGCGCAAATAGATTTGATGTTACAAAACTGCACCATTATGATTGCTC TGTAATGGATTTTTGAAAAGCATTGTGAAATGGAGATGACTTTCCAGTGCTGATGTCAAA AATGCATATGCAGAGTTTAAATATAATTTTGTGTGTTTTGTTTTTTGAGACAGGGTCTTG CTCTGTCTCAAGTGGAGTACAGTGGCGAGATCATGGCTTACCACAGCCTCAACTTCTCAG GCTCCAGCCATCCTCTCACCTCAGCCTCCCAAGTAGCTAGGACTACAGGCGTGTGCCACC ACACCCAGCCAATTTTTGTATTTTTTTGTAGAAATGGGCTTTGGCCATGTTGCCCAGGCT GGCCTCGAACTCCTGAGCCCAAGCAATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATT ACAGGCAGGAGCCACTGTGCCTGGCCTAATTTTATAATTTTAAATGTCTGTAAAGTAAGC AAAATGACTAGTTGAATTGTACATTAAATATATCTAATCCCTAAGAGTTTATAATGGAAA GGGATCAAAACTTTTTCAATTGCGATAAAACTCACATAACAAAATTAACCATTTTAAAGT GAACAATTTAGTGGTATTTAGTGCATTCACAGTGTCGTGCAGCCAACACCTGTATCTAGT TCCAAGACATTTTCATCTCCCCAAAAGGAGACCCCATACACGTGAAGCAGTCCCTCTCCC ATCCCCCTCCTCCCATCCGCTAGCAACTGCTAATTTGCTTTTTGTCTCTATGGATTTACC TGTTCTGGATATTTCACATAAATGGAATTATACAATAAAGGACCGTTTGTTTCTAACTTC TTTCACTAAGCATCCATGTTATAGCATATATCAGACTTCATTCCTTTATGACTGAATAAT ACTTTGCTGAATGGATATATCAGTGTTTTATTTGTCCTTTCATCAGTTGGTGGACATTTG GGTTCTTTCTACCTTTTGGCTATTGTGACTAGTGGTGTTGCAAACATTCATGTACAAGTT TTTGTTTGAATGCCTGTTGTCAGTTCTTCTAGATATATACTTAGAAGTGGAATTGCTGGG TCATATGGTAATTTCTCTGTTTCAACTTTTTGAGAGACCACGAAACCGTTTTCCACAGTG GTGGCACCATTTGACATGCCCACTAGAAATATATGAGAGTTCCATTTTCTTCACATCTTC TGACAGCTTTTTGGGGTGTTGGGATGATTCGTCTAATTGGGAAAATAAGTACCTTATTTG GGCCTATATGGTATTTTTAATTCTGTGTGTCTGCTCCCAAACTCTGTAGCCCTGCCCTCC CTTATATTGTGTATCCTGTTTCAGATTGTTTCCGTTTCTTCTGAGACACTGCATATACCC CTACATGTATCTGTGCTAGGCATACATCCCTGTAACTTCATGGCATTTTTGTAATTGCTG TGTACCTGTTACGTATACTGAGTGGGAGTGTCTCATTTGCTGTAGAACTCTCTGCATTAG TGTAGTGTCTGGCACACAGTAGCTGCTCAGGCATTTTTTAAGTATATGATTATTGGGAAA ACTACAGAGCTAGGGAGAGATCTGAAGAAATACTAGAAATTTTTTTCTGCAAAGAAAACT TTAGAAAATTGTTGACATCATCTGTGGCATGCAGCAAAAGTTGATGTAAAAGGAACAGAA AGACCTATGGAGATAATGGGATGTAATAACAGAAAACATACAGAAACAATTGGTTCTGAA ATCAGTTAAGAGCAGAACTGGAATTCATACTCTGTGGAATGAATCAGCAATGTGGAAGGA GTAACAAGATTTTTTAAAAAGATTTTGAATTTAGGCTGGGTGCAGTGGCTCACGCCTGTG ACCCCATCACTTTGGGAGGCCAGAGTGGGTGGATTGCTTCAGCCCAGGCGTTCGAGACTA GCCTGGACAGCATGGCAAAACCCTATCTCTACAAAAAATAAAAAATTAGCCAGGTGTGGT GGCATGCGCCTGTAGTCCCATCTACTTGGGAGGCTGAGGTGGATGGATCACCTGAGCCTG GGAAGTGGAGGTTGCAGTAGGCTGAAGTCATGCCACTGCACTCAAACCTGGCGGACAGAG TGAGACCCTATCTCAAAAAAAAAAAAAAAAAACTCGAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_014127 unedited NGTTANGATTTGTATACGACTCACTATAGGCGGCCGCGCAATTCGCACGAGGCGCAAATA GATTTGATGTTACAAAACTGCACCATTATGATTGCTCTGTAATGGATTTTNTGAAAAGCA TTGTGAAATGGAGATGACTTTCCAGTGCTGATGTCAAAAATGCATATGCAGAGTTTAAAT ATAATTTTGTGTGTTTTGTTTTTTGAGACAGGGTCTTGCTCTGTCTCAAGTGGAGTACAG TGGCGAGATCATGGCTTACCACAGCCTCAACTTCTCAGGCTCCAGCCATCCTCTCACCTC AGCCTCCCAAGTAGCTAGGACTACAGGCGTGTGCCACCACACCCAGCCAATTTTTGTATT TTTTTGTAGAAATGGGCTTTGGCCATGTTGCCCAGGCTGGCCTCGAACTCCTGAGCCCAA GCAATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCAGGAGCCACTGTGCCT GGCCTAATTTTATAATTTTAAATGTCTGTAAAGTAAGCAAAATGACTAGTTGAATTGTAC ATTAAATATATCTAATCCCTAAGAGTTTATAATGGAAAGGGATCAAAACTTTTTCAATTG CGATAAAACTCACATAACAAAATTAACCATTTTAAAGTGAACAATTTAGTGGTATTTAGT GCATTCACAGTGTCGTGCAGCCAACACCTGTATCTAGTTCCAAGACATTTTCATCTCCCC AAAGGAGACCCCATACACGTGAAGCAGTTCCTCTCCCATCCCCCTCCTTCCATCCGCTAG CAACTGCTAATTTGCTTTTTGTCTCTATGGATTTACCCTGTCTGGATATTCACCATAATG GAATCATCCATAAAGACCGTTTGTTCCTACTTTTTCACTAGCATCCCATGTATAGATATT CAAACTTCTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_014127 unedited TTTGGCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAGAAGGGTCTCA CTCTGTCCGCCAGGTTTGAGTGCAGTGGCATGACTTCAGCCTACTGCAACCTCCACTTCC CAGGCTCAGGTGATCCATCCACCTCAGCCTCCCAAGTAGATGGGACTACAGGCGCATGCC ACCACACCTGGCTAATTTTTTATTTTTTGTAGAGATAGGGTTTTGCCATGCTGTCCAGGC TAGTCTCGAACGCCTGGGCTGAAGCAATCCACCCACTCTGGCCTCCCAAAGTGATGGGGT CACAGGCGTGAGCCACTGCACCCAGCCTAAATTCAAAATCTTTTTAAAAAATCTTGTTAC TCCTTCCACATTGCTGATTCATTCCACAGAGTATGAATTCCAGTTCTGCTCTTAACTGAT TTCAGAACCAATTGTTTCTGTATGTTTTCTGTTATTACATCCCATTATCTCCATAGGTCT TTCTGTTCCTTTTACATCAACTTTTGCTGCATGCCACAGATGATGTCAACAATTTTCTAA AGTTTTCTTTGCAGAAAAAAATTTCTAGTATTTCTTCAGATCTCTCCCTAGCTCTGTAGT TTTCCCAATAATCATATACTTAAAAAATGCCTGAGCAGCTACTGTGTGCCAGACACTACA CTAATGCAGAGAGTTCTACAGCAAATGAGACACTCCCACTCAGTATACGTAACAGGTACA CAGCAATTACAAAAATGCCATGAAGTTACAGGGATGTATGCCTAGCACAGATACATGTAG GGGTATATGCAGTGTCTCANAAGAAACGGAACAATCTGAAACAGATACACATATAGGGAG GGCAGGCTACAGAGTTTGGGAGCCACCACAGATTAAAATACATATGGCCCNNATAGTACT NATTTCCN
Restriction Sites	NotI-NotI
ACCN	NM_014127
Insert Size	2050 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_014127.1, NP_054846.1
RefSeq Size	1784 bp
RefSeq ORF	300 bp
Locus ID	29045
Gene Summary	DISCONTINUED: LocusID 29045 was defined by AF090926.1 and NM_014127.1 which contain L1MB5 repeat sequences and do not appear to represent a protein coding gene.

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