eIF3s8 (EIF3C) (NM_003752) Human Untagged Clone
CAT＃: SC117806
EIF3C (untagged)-Human eukaryotic translation initiation factor 3, subunit C (EIF3C), transcript variant 1
CNY 6696.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | eIF3-p110; EIF3CL; EIF3S8 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117806 sequence for NM_003752 edited (data generated by NextGen Sequencing)
ATGTCGCGGTTTTTCACCACCGGTTCGGACAGCGAGTCCGAGTCGTCCTTGTCCGGGGAG GAGCTCGTCACCAAACCTGTCGGAGGCAACTATGGCAAACAGCCATTGTTGCTGAGCGAG GATGAAGAAGATACCAAGAGAGTTGTCCGCAGTGCCAAGGACAAGAGGTTTGAGGAGCTG ACCAACCTTATCCGGACCATCCGTAATGCCATGAAGATTCGTGATGTCACCAAGTGCCTG GAAGAGTTTGAGCTCCTGGGAAAAGCATATGGGAAGGCCAAAAGCATTGTGGACAAAGAA GGTGTCCCCCGGTTCTATATCCGCATCCTGGCTGACCTAGAGGACTATCTTAATGAGCTT TGGGAAGATAAGGAAGGGAAGAAGAAGATGAACAAGAACAATGCCAAGGCTCTGAGCACC TTGCGTCAGAAGATCCGAAAATACAACCGTGATTTCGAGTCCCATATCACAAGCTACAAG CAGAACCCCGAGCAGTCTGCGGATGAAGATGCTGAGAAAAATGAGGAGGATTCAGAAGGC TCTTCAGATGAGGATGAGGATGAGGACGGAGTCAGTGCTGCAACTTTCTTGAAGAAGAAA TCAGAAGCTCCTTCTGGGGAGAGTCGCAAGTTCCTCAAAAAGATGGATGATGAAGATGAG GACTCAGAAGATTCCGAAGATGATGAAGACTGGGACACAGGTTCCACATCTTCCGATTCC GACTCAGAGGAGGAAGAAGGGAAACAAACCGCGCTGGCCTCAAGATTTCTTAAAAAGGCA CCCACCACAGATGAGGACAAGAAGGCAGCCGAGAAGAAACGGGAGGACAAAGCTAAGAAG AAGCACGACAGGAAATCCAAGCGCCTGGATGAGGAGGAGGAGGACAATGAAGGCGGGGAG TGGGAAAGGGTCCGGGGCGGAGTGCCGTTGGTTAAGGAGAAGCCAAAAATGTTTGCCAAG GGAACTGAGATCACCCATGCTGTTGTTATCAAGAAACTGAATGAGATCCTACAGGCACGA GGCAAGAAGGGAACTGATCGTGCTGCCCAGATTGAGCTGCTGCAACTGCTGGTTCAGATT GCAGCGGAAAACAACCTGGGAGAGGGCGTCATTGTCAAGATCAAGTTCAATATCATCGCC TCTCTCTATGACTACAACCCCAATCTGGCAACCTACATGAAGCCAGAGATGTGGGGGAAG TGCCTGGACTGCATCAATGAGCTGATGGATATCCTGTTTGCAAATCCCAACATTTTTGTT GGAGAGAATATTCTGGAAGAGAGTGAGAACCTGCACAACGCTGACCAGCCACTGCGTGTC CGTGGCTGCATCCTAACTCTGGTGGAACGAATGGATGAAGAATTTACCAAAATAATGCAA AATACTGACCCTCACTCCCAAGAGTACGTGGAGCACTTGAAGGATGAGGCCCAGGTGTGT GCCATCATCGAGCGTGTGCAGCGCTACCTGGAGGAGAAGGGCACTACCGAGGAGGTCTGC CGCATCTACCTGCTGCGCATCCTGCACACCTACTACAAGTTTGATTACAAGGCCCATCAG CGACAGCTGACCCCGCCTGAGGGCTCCTCAAAGTCTGAGCAAGACCAGGCAGAAAATGAG GGCGAGGACTCGGCTGTGTTGATGGAGAGACTGTGCAAGTACATCTACGCCAAGGACCGC ACAGACCGGATCCGCACATGTGCCATCCTCTGCCACATCTACCACCATGCTCTGCACTCG CGCTGGTACCAGGCCCGCGACCTCATGCTCATGAGCCACTTGCAGGACAACATTCAGCAT GCAGACCCGCCAGTGCAGATCCTTTACAACCGCACCATGGTGCAGCTGGGCATCTGTGCC TTCCGCCAAGGCCTGACCAAGGACGCACACAACGCCCTGCTGGACATCCAGTCGAGTGGC CGAGCCAAGGAGCTTCTGGGCCAGGGCCTGCTGCTGCGCAGCCTGCAGGAGCGCAACCAG GAGCAGGAGAAGGTGGAGCGGCGCCGTCAGGTCCCCTTCCACCTGCACATCAACCTGGAG CTGCTGGAGTGTGTCTACCTGGTGTCTGCCATGCTCCTGGAGATCCCCTACATGGCCGCC CATGAGAGCGATGCCCGCCGACGCATGATCAGCAAGCAGTTCCACCACCAGCTGCGCGTG GGCGAGCGACAGCCCCTGCTGGGTCCCCCTGAGTCCATGCGGGAACATGTGGTCGCTGCC TCCAAGGCCATGAAGATGGGTGACTGGAAGACCTGTCACAGTTTTATCATCAATGAGAAG ATGAATGGGAAAGTGTGGGACCTTTTCCCCGAGGCTGACAAAGTCCGCACCATGCTGGTT AGGAAGATCCAGGAAGAGTCACTGAGGACCTACCTCTTCACCTACAGCAGTGTCTATGAC TCCATCAGCATGGAGACGCTGTCAGACATGTTTGAGCTGGATCTGCCCACTGTGCACTCC ATCATCAGCAAAATGATCATTAATGAGGAGCTGATGGCCTCCCTGGACCAGCCAACACAG ACAGTGGTGATGCACCGCACTGAGCCCACTGCCCAGCAGAACCTGGCTCTGCAGCTGGCC GAGAAGCTGGGCAGCCTGGTGGAGAACAACGAACGGGTGTTTGACCACAAGCAGGGCACC TACGGGGGCTACTTCCGAGACCAGAAGGACGGCTACCGCAAAAACGAGGGCTACATGCGC CGCGGTGGCTACCGCCAGCAGCAGTCTCAGACGGCCTACTGA Clone variation with respect to NM_003752.3 1164 c=>t >OriGene 5' read for NM_003752 unedited
GGGTTCAGNATTTGTAAACGATTCACTATAGGCGGCCCGCGAATTCGCACGAGGGCCCTG CGGTGTGACTCGCGGGCTCAGCTGGTCCGGCCGTAGCACCTCCGCGCCGTCGCCATGTCG CGGTTTTTCACCACCGGTTCGGACAGCGAGTCCGAGTCGTCCTTGTCCGGGGAGGAGCTC GTCACCAAACCTGTCGGAGGCAACTATGTTTTTTGCCATTGTTGCTGAGCGAGGATGAAG AAGATACCAAGAGAGTTGTCCGCAGTGCCAAGGACAAGAGGTTTGAGGAGCTGACCAACC TTATCCGGACCATCCGTAATGCCATGAAGATTCGTGATGTCACCAAGTGCCTGGAAGAGT TTGAGCTCCTGGGAAAAGCATATGGGAAGGCCAAAAGCATTGTGGACAAAGAAGGTGTCC CCCGGTTCTATATCCGCATCCTGGCTGACCTAGAGGACTATCTTAATGAGCTTTGGGAAG ATAAGGAAGGGAAGAAGAAGATGAACAAGAACAATGCCAAGGCTCTGAGCACCTTGCGTC AGAAGATCCGAAAATACAACCGTGATTTCGAGTCCCATATCACAAGCTACAAGCAGAACC CCGAGCAGTCTGCGGATGAAGATGCTGAGAAAAATGAGGAGGATTCAGAAGGCTCTTCAG ATGAGGATGAGGATGAGGACGGAGTCAGTGCTGCAACTTTCTTGAAGAAGAAATCACAAG CTCCTTCTGGGGAGAGTCGCAAGTTCCTCAAAAAGATGGATGATGAAGATGACGACTCAG AAGATCCCGAGATGATGAAGACTGGGACACAGTTTCCCATCTTCCGATTCCGACTTCATA GGAGAAGTAGGGAACCAACCGCGCCTGCCTCAGATTTCTTAAAAGCCCCCCCACGAGGAG ACAG >OriGene 3' read for NM_003752 unedited
NNCCAAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCAAC TTTAAACAGACCTTTAGTGACTGAGGTGTGGTTTAGGACTTCAAGGTTGGATGGCCCAGG CGGGAAACAGAGTGGAGAGCTCAGTAGGCCGTCTGAGACTGCTGCTGGCGGTAGCCACCG CGGCGCATGTAGCCCTCGTTTTTGCGGTAGCCGTCCTTCTGGTCTCGGAAGTAGCCCCCG TAGGTGCCCTGCTTGTGGTCAAACACCCGTTCGTTGTTCTCCACCAGGCTGCCCAGCTTC TCGGCCAGCTGCAGAGCCAGGTTCTGCTGGGCAGTGGGCTCAGTGCGGTGCATCACCACT GTCTGTGTTGGCTGGTCCAGGGAGGCCATCAGCTCCTCATTAATGATCATTTTGCTGATG ATGGAGTGCACAGTGGGCAGATCCAGCTCAAACATGTCTGACAGCGTCTCCATGCTGATG GAGTCATAGACACTGCTGTACGTGAAGAGGTAGGTCCTCAGTGACTCTTCCTGGATCTTC CTAACCAGCATGGTGCGGACTTTGTCAGCCTCGGGGAAAAGGTCCCACACTTTCCCATTC ATCTTCTTATTGATGATAAAACTGCGACAGGTCTTCCAGTCACCCATCTTCATGGCCTTG GAGGCCGCGACCACATGTTCCCGCATGGACTCAAGTGGACCCAGCATGGGCTGTCGCTTG CCCACGCGCAGCTGCCGGTGGAACTGCTTGCTGATCATTCGTCCGCGGGCATCGCTCTCA TGGGCGGCCATGTACGGGATCTCCAGGAGCCTGGCAGACCCAGGGTGAAACACTCCAGCC TTCCAGTTGATGTGTAAGTTGTATGGGAACTGTACGGGCCGTTTCACCTTCTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003752 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003752.2, NP_003743.1 |
| RefSeq Size | 3027 bp |
| RefSeq ORF | 2742 bp |
| Locus ID | 8663 |
| UniProt ID | Q99613 |
| Domains | PCI |
| Protein Families | Druggable Genome |
| Gene Summary | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) is the predominant transcript. Variants 1-4 encode the same isoform (a). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221404 | EIF3C (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit C (EIF3C), transcript variant 1 |
CNY 6688.00 |
|
| RC221404L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit C (EIF3C), transcript variant 1, Myc-DDK-tagged |
CNY 9088.00 |
|
| RC221404L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit C (EIF3C), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RC221404L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit C (EIF3C), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC221404L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit C (EIF3C), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG221404 | EIF3C (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit C (EIF3C), transcript variant 1 |
CNY 8288.00 |
