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SSBP1 (NM_003143) Human Untagged Clone
CAT＃: SC118188
SSBP1 (untagged)-Human single-stranded DNA binding protein 1 (SSBP1), nuclear gene encoding mitochondrial protein
CNY 1,800.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | Mt-SSB; mtSSB; SOSS-B1; SSBP |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118188 sequence for NM_003143 edited (data generated by NextGen Sequencing)
ATGTTTCGAAGACCTGTATTACAGGTACTTCGTCAGTTTGTAAGACATGAGTCCGAAACA ACTACCAGTTTGGTTCTTGAAAGATCCCTGAATCGTGTGCACTTACTTGGGCGAGTGGGT CAGGACCCTGTCTTGAGACAGGTGGAAGGAAAAAATCCAGTCACAATATTTTCTCTAGCA ACTAATGAGATGTGGCGATCAGGGGATAGTGAAGTTTACCAACTGGGTGATGTCAGTCAA AAGACAACATGGCACAGAATATCAGTATTCCGGCCAGGCCTCAGAGACGTGGCATATCAA TATGTGAAAAAGGGGTCTCGAATTTATTTGGAAGGGAAAATAGACTATGGTGAATACATG GATAAAAATAATGTGAGGCGACAAGCAACAACAATCATAGCTGATAATATTATATTTCTG AGTGACCAGACGAAAGAGAAGGAGTAG Clone variation with respect to NM_003143.1 >OriGene 5' read for NM_003143 unedited
GGGTCATATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGCGGAGTGA TCCCCTGCGTGGCTGGGCTGCTCGGGTTAGATCGTCAGGAAAAGCCTAAAGATTAGACTG TAAGAAAAGAAAATAGAAGCCATGTTTCGAAGACCTGTATTACAGGTACTTCGTCAGTTT GTAAGACATGAGTCCGAAACAACTACCAGTTTGGTTCTTGAAAGATCCCTGAATCGTGTG CACTTACTTGGGCGAGTGGGTCAGGACCCTGTCTTGAGACAGGTGGAAGGAAAAAATCCA GTCACAATATTTTCTCTAGCAACTAATGAGATGTGGCGATCAGGGGATAGTGAAGTTTAC CAACTGGGTGATGTCAGTCAAAAGACAACATGGCACAGAATATCAGTATTCCGGCCAGGC CTCAGAGACGTGGCATATCAATATGTGAAAAAGGGGTCTCGAATTTATTTGGAAGGGAAA ATAGACTATGGTGAATACATGGATAAAAATAATGTGAGGCGACAAGCAACAACAATCATA GCTGATAATATTATATTTCTGAGTGACCAGACGAAAGAGAAGGAGTAGAAAGGATGATTC TTCTTTGGCCATCATTTGGTACAGTCTCATTTCCAAGTCATGTATAATCTTTATGGCTTC CAAGGACAAGAATTAAAATACTCTTTTACGTAAAAAAAAAAAAAAAAAAAACTCGACTCT AGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGACCCCGGTGGCATTCCTGTGACCC CTTCCCATGCCTCTCCTGGCCCTGAAGTTGCCACTTCAGTGCCCACAGCCTTGTCCTATA AAATTAAGTGCATCATTTTGTCTGACTAGGTGTCCTTTATAATATTATGGGGTGGAGGGG GGGGGT >OriGene 3' read for NM_003143 unedited
GCTATGGACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTACGTAAA AGATATTTTAATTCTTGTCCTTGGAAGCCATAAAGATTATACATGACTTGGAAATGAGAC TGTACCAAATGATGGCCAAAGAAGAATCATCCTTTCTACTCCTTCTCTTTCGTCTGGTCA CTCAGAAATATAATATTATCAGCTATGATTGTTGTTGCTTGTCGCCTCACATTATTTTTA TCCATGTATTCACCATAGTCTATTTTCCCTTCCAAATAAATTCGAGACCCCTTTTTCACA TATTGATATGCCACGTCTCTGAGGCCTGGCCGGAATACTGATATTCTGTGCCATGTTGTC TTTTGACTGACATCACCCAGTTGGTAAACTTCACTATCCCCTGATCGCCACATCTCATTA GTTGCTAGAGAAAATATTGTGACTGGATTTTTTCCTTCCACCTGTCTCAAGACAGGGTCC TGACCCACTCGCCCAAGTAAGTGCACACGATTCAGGGATCTTTCAAGAACCAAACTGGTA GTTGTTTCGGACTCATGTCTTACAAACTGACGAAGTACCTGTAATACAGGTCTTCGAAAC ATGGCTTCTATTTTCTCTTCTTACAGNCTAATCTTTANGCTCTTCCTGACGATCTACCCC GAGCCGCCCACCACGCAGGGGATCACTTCCGCCTNNGGCCGAATCNNGGGCCGCCTATAG TGAGTCGTATTACAAATCCTGACGGGTCACTAAACGAGCCCTGCTTATATAAACCCCCCA CCGGACACGCCCTAACGCCCATTTGTGGTNAACGGGGGGGGGTTATACACANATTCTGAA AAGNCCCGTTGCTTTGGGGCCCAACCAACCCGCCTGGCNNNCTGGCGCGGGGGTGCGAAA TCCNNGAGAGCCCNNCCCTTCGGGCCTTGGCGGGCCGNCAACCCCTACTCGAGTGGCGNG CCGCAACCCAAGCGCGGGCA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003143 |
| Insert Size | 690 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003143.1, NP_003134.1 |
| RefSeq Size | 628 bp |
| RefSeq ORF | 447 bp |
| Locus ID | 6742 |
| UniProt ID | Q04837 |
| Domains | SSB |
| Protein Families | Druggable Genome |
| Protein Pathways | DNA replication, Homologous recombination, Mismatch repair |
| Gene Summary | SSBP1 is a housekeeping gene involved in mitochondrial biogenesis (Tiranti et al., 1995 [PubMed 7789991]). It is also a subunit of a single-stranded DNA (ssDNA)-binding complex involved in the maintenance of genome stability (Huang et al., 2009) [PubMed 19683501].[supplied by OMIM, Feb 2010] Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. Variants 1 through 5 encode the same protein. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Alpers disease mutations in human DNA polymerase gamma cause catalytic defects in mitochondrial DNA replication by distinct mechanisms
,Qian, Y;Ziehr, JL;Johnson, KA;,
Front Genet
,PubMed ID 25914719
[SSBP1]
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