Lymphocyte Activation Gene 3 (LAG3) (NM_002286) Human Untagged Clone
CAT＃: SC118732
LAG3 (untagged)-Human lymphocyte-activation gene 3 (LAG3)
CNY 5872.00
| Cited in 1 publication. |
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CD223 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>SC118732 representing NM_002286.
Blue=Insert sequence Red=Cloning site Green=Tag(s) CTGCTCTCCGCCACGGCCCTGCTCTGTTCCCTGGGACACCCCCGCCCCCACCTCCTCAGGCTGCCTGAT CTGCCCAGCTTTCCAGCTTTCCTCTGGATTCCGGCCTCTGGTCATCCCTCCCCACCCTCTCTCCAAGGC CCTCTCCTGGTCTCCCTTCTTCTAGAACCCCTTCCTCCACCTCCCTCTCTGCAGAACTTCTCCTTTACC CCCCACCCCCCACCACTGCCCCCTTTCCTTTTCTGACCTCCTTTTGGAGGGCTCAGCGCTGCCCAGACC ATAGGAGAGATGTGGGAGGCTCAGTTCCTGGGCTTGCTGTTTCTGCAGCCGCTTTGGGTGGCTCCAGTG AAGCCTCTCCAGCCAGGGGCTGAGGTCCCGGTGGTGTGGGCCCAGGAGGGGGCTCCTGCCCAGCTCCCC TGCAGCCCCACAATCCCCCTCCAGGATCTCAGCCTTCTGCGAAGAGCAGGGGTCACTTGGCAGCATCAG CCAGACAGTGGCCCGCCCGCTGCCGCCCCCGGCCATCCCCTGGCCCCCGGCCCTCACCCGGCGGCGCCC TCCTCCTGGGGGCCCAGGCCCCGCCGCTACACGGTGCTGAGCGTGGGTCCCGGAGGCCTGCGCAGCGGG AGGCTGCCCCTGCAGCCCCGCGTCCAGCTGGATGAGCGCGGCCGGCAGCGCGGGGACTTCTCGCTATGG CTGCGCCCAGCCCGGCGCGCGGACGCCGGCGAGTACCGCGCCGCGGTGCACCTCAGGGACCGCGCCCTC TCCTGCCGCCTCCGTCTGCGCCTGGGCCAGGCCTCGATGACTGCCAGCCCCCCAGGATCTCTCAGAGCC TCCGACTGGGTCATTTTGAACTGCTCCTTCAGCCGCCCTGACCGCCCAGCCTCTGTGCATTGGTTCCGG AACCGGGGCCAGGGCCGAGTCCCTGTCCGGGAGTCCCCCCATCACCACTTAGCGGAAAGCTTCCTCTTC CTGCCCCAAGTCAGCCCCATGGACTCTGGGCCCTGGGGCTGCATCCTCACCTACAGAGATGGCTTCAAC GTCTCCATCATGTATAACCTCACTGTTCTGGGTCTGGAGCCCCCAACTCCCTTGACAGTGTACGCTGGA GCAGGTTCCAGGGTGGGGCTGCCCTGCCGCCTGCCTGCTGGTGTGGGGACCCGGTCTTTCCTCACTGCC AAGTGGACTCCTCCTGGGGGAGGCCCTGACCTCCTGGTGACTGGAGACAATGGCGACTTTACCCTTCGA CTAGAGGATGTGAGCCAGGCCCAGGCTGGGACCTACACCTGCCATATCCATCTGCAGGAACAGCAGCTC AATGCCACTGTCACATTGGCAATCATCACAGTGACTCCCAAATCCTTTGGGTCACCTGGATCCCTGGGG AAGCTGCTTTGTGAGGTGACTCCAGTATCTGGACAAGAACGCTTTGTGTGGAGCTCTCTGGACACCCCA TCCCAGAGGAGTTTCTCAGGACCTTGGCTGGAGGCACAGGAGGCCCAGCTCCTTTCCCAGCCTTGGCAA TGCCAGCTGTACCAGGGGGAGAGGCTTCTTGGAGCAGCAGTGTACTTCACAGAGCTGTCTAGCCCAGGT GCCCAACGCTCTGGGAGAGCCCCAGGTGCCCTCCCAGCAGGCCACCTCCTGCTGTTTCTCATCCTTGGT GTCCTTTCTCTGCTCCTTTTGGTGACTGGAGCCTTTGGCTTTCACCTTTGGAGAAGACAGTGGCGACCA AGACGATTTTCTGCCTTAGAGCAAGGGATTCACCCTCCGCAGGCTCAGAGCAAGATAGAGGAGCTGGAG CAAGAACCGGAGCCGGAGCCGGAGCCGGAACCGGAGCCCGAGCCCGAGCCCGAGCCGGAGCAGCTCTGA CCTGGAGCTGAGGCAGCCAGCAGATCTCAGCAGCCCAGTCCAAATAAACTCCCTGTCAGCAGCAAAAAA AAAAAAAAAAAA >OriGene 5' read for NM_002286 unedited
AATTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCTCTCCGCC ACGGCCCTGCTCTGTTCCCTGGGACACCCCCGCCCCCACCTCCTCAGGCTGCCTGATCTG CCCAGCTTTCCAGCTTTCCTCTGGATTCCGGCCTCTGGTCATCCCTCCCCACCCTCTCTC CAAGGCCCTCTCCTGGTCTCCCTTCTTCTAGAACCCCTTCCTCCACCTCCCTCTCTGCAG AACTTCTCCTTTACCCCCCACCCCCCACCACTGCCCCCTTTCCTTTTCTGACCTCCTTTT GGAGGGCTCAGCGCTGCCCAGACCATAGGAGAGATGTGGGAGGCTCAGTTCCTGGGCTTG CTGTTTCTGCAGCCGCTTTGGGTGGCTCCAGTGAAGCCTCTCCAGCCAGGGGCTGAGGTC CCGGTGGTGTGGGCCCAGGAGGGGGCTCCTGCCCAGCTCCCCTGCAGCCCCACAATCCCC CTCCAGGATCTCAGCCTTCTGCGAAGAGCAGGGGTCACTTGGCAGCATCAGCCAGACAGT GGCCCGCCCGCTGCCGCCCCCGGCCATCCCCTGGCCCCCGGCCCTCACCCGGCGGCGCCC TCCTCCTGGGGGCCCAGGCCCCGCCGCTACACGGTGCTGAGCGTGGGTCCCGGAGGCCTG CGCAGNCGGAGGCTGCCCCTGCAGCCCCGCGTCCAGCTGGATGAGCCGCGCCCCGCAGCG CGGGACTTCTCGCTATGGCTGCGCCCAGCCCCGCGCGCGGACGCCNGCGAGTACCGCGCC CGCGTGCACCTNAGGNNACCGNGCCCTCTCCTGCCGNCTNCGTNTGCGCCTGGGGCCAGC CTCGATGACTGCCAGNCCCCCAGATCTCTCAGACCTCCGACTGGGTCATTNTGACTGCTC CTTAGCGCCTGACGCCCANCCTGTGCATT >OriGene 3' read for NM_002286 unedited
CCGCGGCCGCATTTTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCTGCTGACAGGGAGTTT ATTTGGACTGGGCTGCTGAGATCTGCTGGCTGCCTCAGCTCCAGGTCAGAGCTGCTCCGG CTCGGGCTCGGGCTCGGGCTCCGGTTCCGGCTCCGGCTCCGGCTCCGGTTCTTGCTCCAG CTCCTCTATCTTGCTCTGAGCCTGCGGAGGGTGAATCCCTTGCTCTAAGGCAGAAAATCG TCTTGGTCGCCACTGTCTTCTCCAAAGGTGAAAGCCAAAGGCTCCAGTCACCAAAAGGAG CAGAGAAAGGACACCAAGGATGAGAAACAGCAGGAGGTGGCCTGCTGGGAGGGCACCTGG GGCTCTCCCAGAGCGTTGGGCACCTGGGCTAGACAGCTCTGTGAAGTACACTGCTGCTCC AAGAAGCCTCTCCCCCTGGTACAGCTGGCATTGCCAAGGCTGGGAAAGGAGCTGGGCCTC CTGTGCCTCCAGCCAAGGTCCTGAGAAACTCCTCTGGGATGGGGTGTCCAGAGAGCTCCA CACAAAGCGTTCTTGTCCAGATACTGGAGTCACCTCACAAAGCAGCTTCCCCAGGGATCC ANGTGACCCAAAGGATTTGGGAGTCACTGTGATGATTGCCAATGTGACAGTGGCACTGAG CTGCTGTTCCTGCAGATGGATATGGCACGCGTAAGTCCCACCCTGGGCCTGCCTCACATC CTCTAGTCGAAGGGTAAAGTCGCCATTGTCTTCATCACCAGGAGGTCACGGCCTCCCCCA GNAGGAGTCCCCTTGGCAGTGAAGAAAGACCTGGCTCCCCCCCCCAACAGGCAGCGGCAG GCCAACCCCACCCTGNACCCTGCTCACCGTACACTGNCCAGGGAGTTGGGGGCTCCAGAC CCCCACACGGGGTTTCCCTGAGGAAACCTGGAGCCTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002286 |
| Insert Size | 1944 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002286.4 |
| RefSeq Size | 1991 bp |
| RefSeq ORF | 1944 bp |
| Locus ID | 3902 |
| UniProt ID | P18627 |
| Domains | ig, IG |
| Protein Families | Transmembrane |
| MW | 70.5 kDa |
| Gene Summary | Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Pathological α-synuclein transmission initiated by binding lymphocyte-activation gene 3
,null,
Science (New York, N.Y.)
,PubMed ID 27708076
[LAG3]
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Other Versions
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| RC220269 | LAG3 (Myc-DDK-tagged)-Human lymphocyte-activation gene 3 (LAG3) |
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| RC220269L1 | Lenti ORF clone of Human lymphocyte-activation gene 3 (LAG3), Myc-DDK-tagged |
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| RC220269L2 | Lenti ORF clone of Human lymphocyte-activation gene 3 (LAG3), mGFP tagged |
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| RC220269L3 | Lenti ORF clone of Human lymphocyte-activation gene 3 (LAG3), Myc-DDK-tagged |
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| RC220269L4 | Lenti ORF clone of Human lymphocyte-activation gene 3 (LAG3), mGFP tagged |
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| RG220269 | LAG3 (tGFP-tagged) - Human lymphocyte-activation gene 3 (LAG3) |
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