PDHB (NM_000925) Human Untagged Clone
CAT＃: SC119582
PDHB (untagged)-Human pyruvate dehydrogenase (lipoamide) beta (PDHB), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 3656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PDHBD; PDHE1-B; PDHE1B; PHE1B |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119582 sequence for NM_000925 edited (data generated by NextGen Sequencing)
ATGGCGGCGGTGTCTGGCTTGGTGCGGAGACCCCTTCGGGAGGTCTCCGGGCTGCTGAAG AGGCGCTTTCACTGGACCGCGCCGGCTGCGCTGCAGGTGACAGTTCGTGATGCTATAAAT CAGGGTATGGATGAGGAGCTGGAAAGAGATGAGAAGGTATTTCTGCTTGGAGAAGAAGTT GCCCAGTATGATGGGGCATACAAGGTTAGTCGAGGGCTGTGGAAGAAATATGGAGACAAG AGGATTATTGACACTCCCATATCAGAGATGGGCTTTGCTGGAATTGCTGTAGGTGCAGCT ATGGCTGGGTTGCGGCCCATTTGTGAATTTATGACCTTCAATTTCTCCATGCAAGCCATT GACCAGGTTATAAACTCAGCTGCCAAGACCTACTACATGTCTGGTGGCCTTCAGCCTGTG CCTATAGTCTTCAGGGGGCCCAATGGTGCCTCAGCAGGTGTAGCTGCCCAGCACTCACAG TGCTTTGCTGCCTGGTATGGGCACTGCCCAGGCTTAAAGGTGGTCAGTCCCTGGAATTCA GAGGATGCTAAAGGACTTATTAAATCAGCCATTCGGGATAACAATCCAGTGGTGGTGCTA GAGAATGAATTGATGTATGGGGTTCCTTTTGAATTTCCTCCGGAAGCTCAGTCAAAAGAT TTTCTGATTCCTATTGGAAAAGCCAAAATAGAAAGGCAAGGAACACATATAACTGTGGTT TCCCATTCAAGACCTGTGGGCCACTGCTTAGAAGCTGCAGCAGTGCTATCTAAAGAAGGA GTTGAATGTGAGGTGATAAATATGCGTACCATTAGACCAATGGACATGGAAACCATAGAA GCCAGTGTCATGAAGACAAATCATCTTGTAACTGTGGAAGGAGGCTGGCCACAGTTTGGA GTAGGAGCTGAAATCTGTGCCAGGATCATGGAAGGTCCTGCGTTCAATTTCCTGGATGCT CCTGCTGTTCGTGTCACTGGTGCTGATGTCCCTATGCCTTATGCAAAGATTCTAGAGGAC AACTCTATACCTCAGGTCAAAGACATCATATTTGCAATAAAGAAAACATTAAATATTTAG Clone variation with respect to NM_000925.3 >OriGene 5' read for NM_000925 unedited
GGCGCTGGTNNACCCNCCTTTTTTCCCCCCNNGGGTTCAAATTTGTATACGACTCACTAT AGGCGGCCCGCGAATTCGCACGAGGCCTCGTGCCGAATTCGGCACGAGGGACACGACCAA GATGGCGGCGGTGTCTGGCTTGGTGCGGAGACCCCTTCGGGAGGTCTCCGGGCTGCTGAA GAGGCGCTTTCACTGGACCGCGCCGGCTGCGCTGCAGGTGACAGTTCGTGATGCTATAAA TCAGGGTATGGATGAGGAGCTGGAAAGAGATGAGAAGGTATTTCTGCTTGGAGAAGAAGT TGCCCAGTATGATGGGGCATACAAGGTTAGTCGAGGGCTGTGGAAGAAATATGGAGACAA GAGGATTATTGACACTCCCATATCAGAGATGGGCTTTGCTGGAATTGCTGTAGGTGCAGC TATGGCTGGGTTGCGGCCCATTTGTGAATTTATGACCTTCAATTTCTCCATGCAAGCCAT TGACCAGGTTATAAACTCAGCTGCCAAGACCTACTACATGTCTGGTGGCCTTCAGCCTGT GCCTATAGTCTTCAGGGGGCCCAATGGTGCCTCAGCAGGTGTAGCTGCCCAGCACTCACA GTGCTTTGCTGCCTGGTATGGGCACTGCCCAGGCTTAAAGGTGGTCAGTCCCTGGAATTC AGAGGATGCTAAAGGACTTATTAAATCAGCCATTCGGGATAACAATCCAGTGGTGGTGCT AGAGAATGAATTGATGTATGGNGTTTCCTTTTGAATTTCCTCCGGNAGCTCAGTCAAAAG ATTTTCTGATTCCTATTGGAAAAGCCCAAATAGAAAGGCCAGGGACACATATTACTGTGG GTTTCCCATTCAGGACTGTGGGCCACTGCTTAAAAACTGCCACAGTGCTATCTAAGAAAG AGTGAATGTTGAGTGATAATTTGCGTACCTTTAGACAATGGACATGGAACCATANAACCC TTGTCATGAGAC >OriGene 3' read for NM_000925 unedited
ACTTCCAGGGCCGGNAGAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTC AGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTA AACGTATATTATATGCTTTAATTTTATACATATAAGTTATCTTGTTTGGATACATCTTTC ATCAGGACTCTGCCACATCCATACTTTGTCCTTGTGGTGAGAGAGGATAAAATGTTATAT AATTTGTTATTCAAAGAACATGGCAACCGTAACAGACAAAAGGAAGCATGGCATCTAGGG GAGGAGAGTGGAGAGTTTTCCATACACAAACACATTTAAACTTCCCTGTACAAAAATACC TGCTGTTGCTTTAGAAATCATTTTCCGTTATGAATAGTCAGGTCTTGCAGTACAAATCCA GGNNCAGTGCCAGCAAGTATTTCAAATAAATTTCAACGACTTGATATTCAAGTCCAAACT AAATATTTAATGTTTTCTTTATTGCAAATATGATGTCTTTGACCTGAGGTATAGAGTTGT CCTCTAGAATCTTTGCATAAGGCATAGGGACATCAGCACCAGTGACACGAACAGCAGGAG CATCCAGGAAATTGAACGCAGGACCTTCCATGATCCTGGCACAGATTTCAGCTCCTACTC CAAACTGTGGCCAGCCTCCTTCCACAGTTACAAGATGATTTTGTCTTCATGACACTGGCT TCTATGGTTTCCATGTCCATTGGTCTAATGNNNNNCATATTTATCACCTCACATTNCACT NCTTCTTAGATAGCACTGCTGCAGCTTCTAGCAGTGGCCCACAGGTCTTGATGGGNAAAC CCAGNTATATGNNGTTCCTTGCCTTTCTATTTTGGNCTTTCCAATANGATCANAAAACTN TTGACTGAGCTTCCGGAGAANTCAAAAGGACCCCATACATAATCATTCTCTACCACACAC TGGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000925 |
| Insert Size | 1530 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000925.1, NP_000916.1 |
| RefSeq Size | 1501 bp |
| RefSeq ORF | 1080 bp |
| Locus ID | 5162 |
| UniProt ID | P11177 |
| Domains | transket_pyr, transketolase_C |
| Protein Pathways | Butanoate metabolism, Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine biosynthesis |
| Gene Summary | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 beta subunit. Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (1) represents the predominant transcript and encodes the longest isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201230 | PDHB (Myc-DDK-tagged)-Human pyruvate dehydrogenase (lipoamide) beta (PDHB), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 3990.00 |
|
| RC201230L3 | Lenti-ORF clone of PDHB (Myc-DDK-tagged)-Human pyruvate dehydrogenase (lipoamide) beta (PDHB), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5890.00 |
|
| RC201230L4 | Lenti-ORF clone of PDHB (mGFP-tagged)-Human pyruvate dehydrogenase (lipoamide) beta (PDHB), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5890.00 |
|
| RG201230 | PDHB (tGFP-tagged) - Human pyruvate dehydrogenase (lipoamide) beta (PDHB), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 4370.00 |
