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HSD17B1 (NM_000413) Human Untagged Clone
CAT＃: SC119873
HSD17B1 (untagged)-Human hydroxysteroid (17-beta) dehydrogenase 1 (HSD17B1)
CNY 2,400.00
CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | 17-beta-HSD; 20-alpha-HSD; E2DH; EDH17B2; EDHB17; HSD17; SDR28C1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119873 sequence for NM_000413 edited (data generated by NextGen Sequencing)
ATGGCCCGCACCGTGGTGCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGCACTTGGCC GTACGTCTGGCTTCAGATCCATCCCAGAGCTTCAAAGTGTATGCCACGTTGAGGGACCTG AAAACACAGGGCCGGCTGTGGGAGGCGGCCCGGGCCCTGGCATGCCCTCCGGGATCCCTG GAGACGTTGCAGCTGGACGTAAGGGACTCAAAATCCGTGGCCGCTGCCCGGGAACGCGTG ACTGAGGGCCGCGTGGACGTGCTGGTGTGTAACGCAGGCCTGGGCCTGCTGGGGCCGCTG GAGGCGCTGGGGGAGGACGCCGTGGCCTCTGTGCTGGACGTGAATGTAGTAGGGACTGTG CGGATGCTGCAGGCCTTCCTGCCAGACATGAAGAGGCGCGGTTCGGGACGCGTGTTGGTG ACCGGGAGCGTGGGAGGATTGATGGGGCTGCCTTTCAATGACGTTTATTGCGCCAGCAAG TTCGCGCTCGAAGGCTTATGCGAGAGTCTGGCGGTTCTGCTGCTGCCCTTTGGGGTCCAC TTGAGCCTGATCGAGTGCGGCCCAGTGCACACCGCCTTCATGGAGAAGGTGTTGGGCAGC CCAGAGGAGGTGCTGGACCGCACGGACATCCACACCTTCCACCGCTTCTACCAATACCTC GCCCACAGCAAGCAAGTCTTTCGCGAGGCGGCGCAGAACCCTGAGGAGGTGGCGGAGGTC TTCCTCACCGCTTTGCGCGCCCCGAAGCCGACCCTGCGCTACTTCACCACCGAGCGCTTC CTGCCCCTGCTGCGGATGCGCCTGGACGACCCCAGCGGCTCCAACTACGTCACCGCCATG CACCGGGAAGTGTTCGGCGACGTTCCGGCAAAGGCCGAGGCTGGGGCCGAGGCTGGGGGC GGGGCCGGGCCTGGGGCAGAGGACGAGGCCGGGCGCGGTGCGGTGGGGGACCCTGAGCTC GGCGATCCTCCGGCCGCCCCGCAGTAA Clone variation with respect to NM_000413.2 >OriGene 5' read for NM_000413 unedited
ATAATTTGTAATACGACTCACTATAGGCGGCCGCGACATTCGCACCAGCTACAACCCACA ATCAGATTATAGTCCTGCCTTGGGCAGCTGAAAAAGGACAGGAGAAGGTCAGAGAGACGA AAGGCTGTTTTTTGAGGCCTGAGGCACCCCAACATGACAACGTAAGACTGTAACCATGGT CATGTGAGTTATGAGCTAGGAACCCTGGACGAAACCAGCACATATACAATCATCTCCCAA CTCCCAACGCCTTTACTTTCACAAGCCTCTGCAGCAAACTGCGGTCACTATAATCGCTCC TGTGGCACATAGGCATACCCAGGGGAATCTGCCCAGGGGGCCACTCTGTGCCCACGTGGG AACCCACACCTGCTTGTAAAGCCTCCCCTCCCTCTGACCAGCAACCAGGACAGTTTGTTG TTCCAAGCAGTGGGCTCATGTCTGTTTTGGCTCAAAACAGGGTGGGGAGAAGCGGGCCAG GGACCCGCATGAAGGCTTATCCTTGAGAATGCGTGGGAAACACAACAAGGGGTGGGGGCC CGCATGCGGNGCGGGGCGAATCATGTGATATCAAGCCCAGAGCCCCAGCCTCTCCCCACA GTCTCACCATGGCCCGCACCGTGGTGCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGC ACTTGGCCGTACGTCTGGCTTCAGAACCATCCCAGAGCTTCAAAGTGTATGCCACGTTGA GGGACCTGAAACACANNNGCCGCTGTGGGATGCGGNCCNGGCCCTGCATGCCTNCGGNAT CCCTGGAAACGTGACNCTGACGTAAGGACTCANAATCGTGNCGCTGCCAGGAATCCTGAC TGAGGCGNNNTGACTGCTTGTGNAAAACCAAGCTGGCCTGCTGGGCCCTGAAGCCTTGGG GAAAGAACCTGGCTCTGCCTGAACTAATTNNANNNAGACTGGCGAATCTGAAGCTTCTGC ACATAAAAAAGCCGTGGGAACANNTTTTGACCGAAACGGGAGAAAATTAA >OriGene 3' read for NM_000413 unedited
GATCGAGTTATTTCCCTTTTTTTTTTTTTGATTTTTAAATTTTCTTTATTTTTGTAGAGG TGGGGGTCTCACTGTGTTGCTCTGGCTGGTCTCCAACTCCGGGGCTTGAGCGATCCTCCT GCCTCCGCCTCCCAAAGCGCTGGCATTACAGGCGACAGCCATCGCGCCTAGCTGCGCTGG TAAAACTGGCTAACGCGATCTATCTTAATTAGCCACCCACAGCTGCTACCGCCGCCCCAT CCCCAGGGACCACACAGACCCAGGGGACAAAGAAGGGCGCGGGAGACAGCGGCTGAGGAA GCCTTTACTGCGGGGCGGCCGGAGGATCGCCGAGCTCAGGGTCCCCCACCGCACCGCGCC CGGCCTCGTCCTCTGCCCCAGGCCCGGCCCCGCCCCCAGCCTCGGCCCCAGCCTCGGCCT TTGCCGGAACGTCGCCGAACACTTCCCGGTGCATGGCGGTGACCTATTTGGAGCCGCCTG GGTCGTTCAGGCGCATCCGCACCAGGGGCAAGAAGCGCTCGGGGGTGAAGTATCGCACGG TCGGCTTTGGCGCGCGCCAAGCGGTGAGGAAGACCTCCGCCACCTCCTCTAGGTTCTGCG CCGCCCTGCCGAAAGACTTGCCTTGCTGTGGCGCCGGTATTGGCCAGAACCCGGTGGAAG GTGCGGGATCTTCCTGTCGGGCCATCCCCCCTCTGCTGGGGTTGCCCAACCACCTTTTTC CTTGAAGGCGGGTGTTGCCTGGGCCCCGCCTCCCCCCAGGCTCAAGCTTCCGGACCCCAA AGGGCCCCCTCTCTCACCCCCCCGATCTCTTGTCATAACCCCCCGCGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000413 |
| Insert Size | 2000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000413.1, NP_000404.1 |
| RefSeq Size | 2230 bp |
| RefSeq ORF | 987 bp |
| Locus ID | 3292 |
| UniProt ID | P14061 |
| Domains | adh_short |
| Protein Families | Druggable Genome |
| Protein Pathways | Androgen and estrogen metabolism, Metabolic pathways |
| Gene Summary | This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016] Transcript Variant: This variant (1) uses an alternate in-frame splice site in the 3' coding region, compared to variant 2. It encodes isoform 1, which is shorter than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211135 | HSD17B1 (Myc-DDK-tagged)-Human hydroxysteroid (17-beta) dehydrogenase 1 (HSD17B1) |
CNY 3,600.00 |
|
| RC211135L1 | Lenti-ORF clone of HSD17B1 (Myc-DDK-tagged)-Human hydroxysteroid (17-beta) dehydrogenase 1 (HSD17B1) |
CNY 6,000.00 |
|
| RC211135L2 | Lenti-ORF clone of HSD17B1 (mGFP-tagged)-Human hydroxysteroid (17-beta) dehydrogenase 1 (HSD17B1) |
CNY 5,890.00 |
|
| RC211135L3 | Lenti-ORF clone of HSD17B1 (Myc-DDK-tagged)-Human hydroxysteroid (17-beta) dehydrogenase 1 (HSD17B1) |
CNY 5,890.00 |
|
| RC211135L4 | Lenti-ORF clone of HSD17B1 (mGFP-tagged)-Human hydroxysteroid (17-beta) dehydrogenase 1 (HSD17B1) |
CNY 5,890.00 |
|
| RG211135 | HSD17B1 (tGFP-tagged) - Human hydroxysteroid (17-beta) dehydrogenase 1 (HSD17B1) |
CNY 5,200.00 |
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