SPSB4 (NM_080862) Human Untagged Clone
CAT＃: SC120350
SPSB4 (untagged)-Human splA/ryanodine receptor domain and SOCS box containing 4 (SPSB4)
CNY 2400.00
CNY 6270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SSB-4; SSB4 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_080862, the custom clone sequence may differ by one or more nucleotides
ATGGGCCAGAAGCTCTCGGGGAGCCTCAAGTCAGTGGAGGTGCGAGAGCCGGCGCTGCGGCCGGCCAAGC GGGAGCTGCGGGGTGCAGAGCCCGGGCGGCCGGCGCGGCTGGACCAGCTGTTGGACATGCCAGCGGCGGG GCTGGCTGTGCAGCTGCGGCACGCGTGGAACCCCGAGGACCGCTCGCTCAACGTCTTCGTCAAGGACGAC GACCGGCTCACCTTCCACCGGCACCCCGTGGCCCAGAGCACCGACGGCATCCGCGGCAAGGTGGGCCACG CCCGCGGCCTGCACGCCTGGCAGATCAACTGGCCGGCTCGGCAGCGCGGCACCCACGCTGTAGTTGGTGT GGCCACGGCCCGTGCTCCCCTGCACTCCGTGGGCTACACGGCGCTGGTAGGCAGTGACGCCGAGTCGTGG GGCTGGGACCTGGGCCGCAGCCGCCTCTACCACGACGGCAAGAACCAGCCCGGCGTGGCCTACCCGGCCT TTCTGGGGCCCGACGAGGCCTTTGCGCTGCCCGACTCGCTGCTCGTGGTGCTGGACATGGATGAGGGCAC ACTCAGCTTCATCGTGGATGGCCAGTACCTGGGCGTGGCCTTCCGAGGTCTCAAGGGCAAGAAGCTGTAC CCGGTGGTGAGTGCCGTGTGGGGCCACTGTGAAGTCACCATGCGCTACATCAACGGCCTTGACCCCGAGC CCCTGCCACTGATGGACCTGTGCCGGAGATCCATCCGCTCGGCCCTGGGCCGCCAGCGCCTGCAGGACAT CAGCTCCCTGCCCCTGCCTCAGTCTCTCAAAAACTATCTGCAGTACCAGTGA >OriGene 5' read for NM_080862 unedited
TTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGAGCAAAGAGACCGACTT CCTGCGCCTCGGGTGGGCGGGGAACGCTCCTTGAGCAGGAGCTTGGGCCCCTGCCGCAGC CCGGTAGAGGCTGTGGAGGTCTACCGTCCGGAAGCCTGGTTCCCAGCCCCGTGGCCCATT CCTGGCTACGGGGAGTGGAGGCTCCCACGAGGTAGCGGTGGCCTGCAGCGGCCTCCTCCC CGCAGTGAAGCATGGGCCAGAAGCTCTCGGGGAGCCTCAAGTCAGTGGAGGTGCGAGAGC CGGCGCTGCGGCCGGCCAAGCGGGAGCTGCGGGGTGCAGAGCCCGGGCGGCCGGCGCGGC TGGACCAGCTGTTGGACATGCCAGCGGCGGGGCTGGCTGTGCAGCTGCGGCACGCGTGGA ACCCCGAGGACCGCTCGCTCAACGTCTTCGTCAAGGACGACGACCGGCTCACCTTCCACC GGCACCCCGTGGCCCAGAGCACCGACGGCATCCGCGGCAAGGTGGGCCACGCCCGCGGCC TGCACGCCTGGCAGATCAACTGGCCGGCTCGGCAGCGCGGCACCCACGCTGTAGTTGGTG TGGCCACGGCCCGTGCTCCCCTGCACTCCGTGGGCTACACGGCGCTGGTAGGCAGTGACG CCGAGTCGTGGGGCTGGGACCTGGGCCGCAGCCGCCTCTACCACGACGGCAAGAACCAGC CCGGCGTGGCCTACCCGGCCTTTCTGGGGCCCGACGAGGCCTTTGCGCTGCCCGACTCGC TGCTCGTGGTGCTGGACATGGATGAGGGCACACTCAGCTTCATCGTGGATGGCCAGTACC TGGGCGTGGCCTTCCGAGGTCTCAAGGGCAGAGCTGT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_080862 |
| Insert Size | 4350 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_080862.1, NP_543138.1 |
| RefSeq Size | 2455 bp |
| RefSeq ORF | 822 bp |
| Locus ID | 92369 |
| UniProt ID | Q96A44 |
| Domains | SPRY |
| Protein Families | Druggable Genome |
| Gene Summary | Substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:21199876, PubMed:15601820). Negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2 (PubMed:21199876). Acts as a bridge which links NOS2 with the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (PubMed:21199876). Diminishes EphB2-dependent cell repulsive responses by mediating the ubiquitination and degradation of EphB2/CTF2 (PubMed:28931592). Regulates cellular clock function by mediating the ubiquitin/proteasome-dependent degradation of the circadian transcriptional repressor NR1D1 (PubMed:26392558).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203566 | SPSB4 (Myc-DDK-tagged)-Human splA/ryanodine receptor domain and SOCS box containing 4 (SPSB4) |
CNY 2400.00 |
|
| RC203566L3 | Lenti ORF clone of Human splA/ryanodine receptor domain and SOCS box containing 4 (SPSB4), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC203566L4 | Lenti ORF clone of Human splA/ryanodine receptor domain and SOCS box containing 4 (SPSB4), mGFP tagged |
CNY 5890.00 |
|
| RG203566 | SPSB4 (tGFP-tagged) - Human splA/ryanodine receptor domain and SOCS box containing 4 (SPSB4) |
CNY 4370.00 |
