E2F8 (BC028244) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	E2F family member 8; E2F transcription factor 8; FLJ23311
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC028244 edited CCACGCGTCCGTACTCCCTCTGATTTGGAGGTGAGACGGTCTTCAAAAGAGAACTGTGCC AAAAACCTCTTTTCCACACGTGGGAAACCAAACTTTACTCGACACCCATCTCTTATCAAA TTGGTAAAGAGTATAGAAAGTGATCGGAGAAAGATAAATTCTGCGCCCAGTAGCCCTATC AAGACCAACAAAGCTGAGAGTTCTCAGAATTCTGCACCCTTCCCAAGTAAAATGGCTCAG CTTGCAGCTATTTGTAAAATGCAGTTAGAAGAGCAATCAAGTGAATCCAGACAGAAAGTG AAAGTACAGCTGGCAAGATCTGGACCCTGCAAACCAGTAGCCCCTCTGGACCCCCCAGTG AATGCTGAGATGGAGCTGACAGCACCGTCCCTCATCCAGCCCCTGGGAATGGTTCCCCTG ATCCCCAGCCCCTTGTCATCAGCAGTGCCCCTGATCCTACCTCAGGCCCCTTCAGGCCCA TCCTATGCCATCTACCTGCAGCCCACTCAAGCCCACCAAAGTGTGACGCCACCCCAAGGC CTGAGCCCAACGGTGTGCACCACCCACTCTTCTAAAGCTACTGGCTCAAAAGACTCCACA GATGCCACCACTGAGAAGGCAGCCAATGATACCTCAAAGGCCAGTGCCTCTACCAGGCCT GGAAGCTTGCTGCCAGCACCAGAGAGGCAAGGGGCAAAGAGCCGAACCAGGGAGCCAGCT GGAGAAAGAGGCTCAAAGAGGGCAAGCATGCTCGAGGACAGTGGTTCCAAAAAGAAATTT AAAGAGGACCTAAAAGGACTTGAAAATGTCTCCGCAACCTTGTTCCCATCAGGATACCTA ATCCCTCTCACGCAGTGCTCATCCCTGGGGGCAGAGTCCATTTTGTCTGGTAAAGAAAAC TCAAGTGCTCTTTCCCCAAACCACAGGATTTACAGCTCCCCAATTGCAGGTGTTATTCCA GTGACATCATCTGAACTCACTGCTGTTAATTTTCCCTCTTTTCATGTAACACCGTTGAAG CTAATGGTCTCACCAACTTCCGTGGCAGCCGTACCTGTCGGGAACAGCCCGGCTCTCGCT TCAAGCCACCCTGTTCCCATCCAGAACCCAAGCTCAGCCATTGTAAACTTCACCCTGCAG CACCTGGGACTCATCTCACCCAATGTGCAGTTGTCTGCCAGCCCTGGGTCTGGAATCGTT CCTGTGTCTCCAAGAATAGAGTCTGTTAATGTCGCACCAGAAAATGCAGGCACTCAGCAA GGAAGGGCCACCAACTATGACTCACCAGTCCCAGGCCAGAGCCAGCCAAATGGACAATCA GTTGCTGTGACAGGGGCACAACAGCCTGTTCCTGTGACACCCAAAGGGTCACAATTAGTG GCCGAAAGTTTCTTCCGTACCCCAGGTGGACCCACCAAGCCAACCAGCTCATCCTGCATG GATTTTGAGGGTGCTAATAAAACCTCCTTAGGAACTCTCTTTGTCCCACAGCGAAAACTG GAAGTCTCAACAGAGGATGTCCATTAATCAACAGATGTTGGCTTAGTTTAATTTTCTAAA GAGTTGTTTAATAGAGAAAATGTACACAGACTGATTTGGAGAACACATTCTCTGAAAATA CTGTAAATACGTTGGGGATTTGTTCAATGTGAAATCAGATAGTTGTTTTCATACATATAT ATATATACACACACACACACACACACACACACACATATATTTGTATAAAGCTAAGTTTAG CTTTCAATCCTACAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC028244 unedited ACGTACAAAATTTGTAATACGACTCACTATAGGGNNCGGCCGCGNAATTNNCTGCACCCT TCCCAAGTAAAATGGCTCAGCTTGCAGCTATTTGTAAAATGCAGTTAGAAGAGCAATCAA GTGAATCCAGACAGAAAGTGAAAGTACAGCTGGCAAGATCTGGACCCTGCAAACCAGTAG CCCCTCTGGACCCCCCAGTGAATGCTGAGATGGAGCTGACAGCACCGTCCCTCATCCAGC CCCTGGGAATGGTTCCCCTGATCCCCAGCCCCTTGTCATCAGCAGTGCCCCTGATCCTAC CTCAGGCCCCTTCAGGCCCATCCTATGCCATCTACCTGCAGCCCACTCAAGCCCACCAAA GTGTGACGCCACCCCAAGGCCTGAGCCCAACGGTGTGCACCACCCACTCTTCTAAAGCTA CTGGCTCAAAAGACTCCACAGATGCCACCACTGAGAAGGCAGCCAATGATACCTCAAAGG CCAGTGCCTCTACCAGGCCTGGAAGCTTGCTGCCAGCACCAGAGAGGCAAGGGGCAAAGA GCCGAACCAGGGAGCCAGCTGGAGAAAGAGGCTCAAAGAGGGCAAGCATGCTCGAGGACA GTGGTTCCAAAAAGAAATTTAAAGAGGACCTAAAAGGACTTGAAAATGTCTCCGCAACCT TGTTCCCATCAGGATACCTAATCCCTCTCACGCAGTGCTCATCCCTGGGGGCAGAGTCCA TTTTGTCTGGTAAAGAANACTCAAGTGCTCTTTTCCCANACCACAGGATTTACAGCTCCC CAATTGCAGGTGTTATTCCAGTGACATCATCTGAACTCACTGCTGTTAATTTTCCCTCTT TTATGTAACACCGTTGAAGCTAATGGTCTCACCAACTTCCGTGGCAGCCGTACCTGTCGG GAACAGCCCCGCTCTCGCTTTCAA
Restriction Sites	Please inquire
ACCN	BC028244
Insert Size	1768 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC028244.1, AAH28244.2
RefSeq Size	1768 bp
Locus ID	79733
Protein Families	Druggable Genome
Gene Summary	This gene encodes a member of a family of transcription factors which regulate the expression of genes required for progression through the cell cycle. The encoded protein regulates progression from G1 to S phase by ensuring the nucleus divides at the proper time. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2012]

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