TRIM47 (BC017299) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	gene overexpressed in astrocytoma; GOA; RNF100; tripartite motif-containing 47
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC017299 edited CTCGAGCGCTACTGCCGCGCGGAGCGCGTGTGTCTGTGCGAGGCCTGCGCCGCACAGGAG CACCGCGGCCACGAGCTGGTGCCGCTGGAGCAGGAGCGCGCGCTTCAGGAGGTGGGCTCC GGGGGCACACTCGGGCTGAGCAGTCCAAAGTCCTGAGCGCCGTGGAGGACCGCATGGACG AGCTGGGTGCTGGCATTGCACAGTCCAGGCGCACAGTGGCCCTCATCAAGAGTGCAGCCG TAGCAGAGCGGGAGAGGGTGAGCCGGCTGTTTGCAGATGCTGCGGCCGCCCTGCAGGGCT TCCAGACCCAGGTGCTGGGCTTCATCGAGGAGGGGGAAGCTGCCATGCTAGGCCGCTCCC AGGGTGACCTGCGGCGACAGGAGGAACAGCGCAGCCGCCTGAGCCGAGCCCGCCAGAATC TCAGCCAGGTCCCTGAAGCTGACTCAGTCAGCTTCCTGCAGGAGCTGCTGGCACTAAGGC TGGCCCTGGAGGATGGGTGTGGCCCTGGGCCTGGACCCCCGAGGGAGCTCAGCTTCACCA AATCATCCCAAGCTGTCCGTGCAGTGAGAGACATGCTGGCCGTGGCCTGCGTCAACCAGT GGGAGCAGCTGAGGGGGCCGGGTGGCAACGAGGATGGGCCACAGAAGCTGGACTCGGAAG CTGATGCTGAGCCCCAAGACCTCGAGAGTACGAACCTCTTGGAGAGTGAAGCTCCCAGGG ACTATTTCCTCAAGTTTGCCTATATTGTGGATTTGGACAGCGACACAGCAGACAAGTTCC TGCAGCTGTTTGGAACCAAAGGTGTCAAGAGGGTGCTGTGTCCTATCAACTACCCCTTGT CGCCCACCCGCTTCACCCATTGTGAGCAGGTGCTGGGCGAGGGTGCCCTGGACCGAGGCA CCTACTACTGGGAGGTGGAGATTATCGAGGGCTGGGTCAGCATGGGGGTCATGGCCGAAG ACTTCTCCCCACAAGAGCCCTACGACCGCGGCCGGCTGGGCCGCAACGCCCACTCCTGCT GCCTGCAGTGGAATGGACGCAGCTTCTCCGTCTGGTTTCATGGGCTGGAGGCTCCCCTGC CCCACCCCTTCTCGCCCACGGTTGGGGTCTGCCTGGAATACGCTGACCGTGCCTTGGCCT TCTATGCTGTACGGGACGGCAAGATGAGCCTCCTGCGGAGGCTGAAGGCCTCCCGGCCCC GCCGGGGTGGCATCCCGGCCTCCCCCATTGACCCCTTCCAGAGCCGCCTGGACAGTCACT TTGCGGGGCTCTTCACCCACAGACTCAAGCCTGCCTTCTTCCTGGAGAGTGTGGACGCCC ACTTGCAGATCGGGCCCCTCAAGAAGTCCTGCATATCCGTGCTGAAGAGGAGGTGATGCC GGGCACGGGCGCTCCTGCTGCCGTCTCTGCTCCAGGAAGCTGCCTCCTCTGGGCCCTCTC CTTCGTCTGGGAAGGCACCAGCATGAGTCCCACACACCCAGCCTTCTCATTTCTAGAGGC TTCCACCTTTTTATACACTCAGCCTTCCCTCTCCCAGGCAGGAGGACCCCCAGACCCTGT TCCCCTGCAGACCTCACTTCTGGGAGACAGAGCTACAGCTGGGACAGCTCCAAGCTACCC TAACCCCTCCTTTCCCAGGTTTCTAGAATAGTGTCTGGCATGTAGTAGATGCTCAATAAA CACTTGTTGAATGAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC017299 unedited NNNNCATGTCAAATATTGTAATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCT CGAGCGCTACTGCCGCGCGGAGCGCGTGTGTCTGTGCGAGGCCTGCGCCGCACAGGAGCA CCGCGGCCACGAGCTGGTGCCGCTGGAGCAGGAGCGCGCGCTTCAGGAGGTGGGCTCCGG GGGCACACTCGGGCTGAGCAGTCCAAAGTCCTGAGCGCCGTGGAGGACCGCATGGACGAG CTGGGTGCTGGCATTGCACAGTCCAGGCGCACAGTGGCCCTCATCAAGAGTGCAGCCGTA GCAGAGCGGGAGAGGGTGAGCCGGCTGTTTGCAGATGCTGCGGCCGCCCTGCAGGGCTTC CAGACCCAGGTGCTGGGCTTCATCGAGGAGGGGGAAGCTGCCATGCTAGGCCGCTCCCAG GGTGACCTGCGGCGACAGGAGGAACAGCGCAGCCGCCTGAGCCGAGCCCGCCAGAATCTC AGCCAGGTCCCTGAAGCTGACTCAGTCAGCTTCCTGCAGGAGCTGCTGGCACTAAGGCTG GCCCTGGAGGATGGGTGTGGCCCTGGGCCTGGACCCCCGAGGGAGCTCAGCTTCACCAAA TCATCCCAAGCTGTCCGTGCAGTGAGAGACATGCTGGCCGTGGCCTGCGTCAACCAGTGG GAGCAGCTGAGGGGGCCGGGTGGCAACGAGGATGGGCCACAGAAGCTGGACTCGGAAGCT GATGCTGAGCCCCAAGACCTCGAGAGTACGAACCTCTTGGAGAGTGAAGCTCCCAGGACT ATTTCCTCAGTTTGCCTATATTGTGGATTTGGACAGCGACACAGCAGACAAGTTCCTGCA GCTGTTAGGACCAAAGTGTCAAGAGGGTGCTGTGTCCTATCAACTACCCCTTGTCGNCCA CC
Restriction Sites	Please inquire
ACCN	BC017299
Insert Size	1721 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC017299.2, AAH17299.1
RefSeq Size	1712 bp
Locus ID	91107
Protein Families	Druggable Genome
Gene Summary	E3 ubiquitin-protein ligase that mediates the ubiquitination and proteasomal degradation of CYLD.[UniProtKB/Swiss-Prot Function]

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