TAF8 (BC033728) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	II; TAF; TAFII-43; TAFII43; TBN
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC033728 edited CGCGCTCGCGCACACTACGCCAGAACAAGATGGCCGACGCGGCGGCCACAGCTGGGGCCG GTGGCTCCGGAACGAGATCGGGAAGTAAACAGTCCACTAACCCTGCCGATAACTATCATC TGGCCCGGAGGAGAACCCTGCAGGTGGTTGTGAGCTCCTTGCTGACAGAGGCAGGGTTTG AGAGTGCCGAGAAAGCATCCGTGGAAACGCTGACAGAGATGCTGCAGAGCTACATTTCAG AAATTGGGAGAAGTGCCAAGTCTTACTGTGAGCACACAGCCAGGACCCAGCCCACACTGT CCGATATCGTGGTCACACTTGTTGAGATGGGTTTCAATGTGGACACTCTCCCTGCTTATG CAAAACGGTCTCAGAGGATGGTCATCACTGCTCCTCCGGTGACCAATCAGCCAGTGACCC CCAAGGCCCTCACTGCAGGGCAGAACCGACCCCACCCGCCGCACATCCCCAGCCATTTTC CTGAGTTCCCTGATCCCCACACCTACATCAAAACTCCGGTGAGTGATGAAGCACTGGGAC TGCGCGTGGTGTAAAGCACGGAGTCAGTTCCTACTGACTGTCACGTGGCAGAGTCCAGTC CAAAAGCTTGTTGATGAAAGAGTGGTTCAAAACTCAGAATGTGGCCGGGCACATTGGCTC ACACCTGTGATCCCAGCATTTTAGGAGGCCGAGGCAGGAGGATCACTTGAGCCTGGGAGT TCAAGACCAGCCTGGGCAGCAGAGCAAGACCCTGTCTCATTTGAAAAATAAATAAATAAA GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC033728 unedited NNNGGTCGTGACGTACAAATTTTGTATACGACTCATATAGGGCGGCCGCGATTCCCGGGA TCGCGCTCGCGCACACTACGCCAGAACAAGATGGCCGACGCGGCGGCCACAGCTGGGGCC GGTGGCTCCGGAACGAGATCGGGAAGTAAACAGTCCACTAACCCTGCCGATAACTATCAT CTGGCCCGGAGGAGAACCCTGCAGGTGGTTGTGAGCTCCTTGCTGACAGAGGCAGGGTTT GAGAGTGCCGAGAAAGCATCCGTGGAAACGCTGACAGAGATGCTGCAGAGCTACATTTCA GAAATTGGGAGAAGTGCCAAGTCTTACTGTGAGCACACAGCCAGGACCCAGCCCACACTG TCCGATATCGTGGTCACACTTGTTGAGATGGGTTTCAATGTGGACACTCTCCCTGCTTAT GCAAAACGGTCTCAGAGGATGGTCATCACTGCTCCTCCGGTGACCAATCAGCCAGTGACC CCCAAGGCCCTCACTGCAGGGCAGAACCGACCCCACCCGCCGCACATCCCCAGCCATTTT CCTGAGTTCCCTGATCCCCACACCTACATCAAAACTCCGGTGAGTGATGAAGCACTGGGA CTGCGCGTGGTGTAAAGCACGGAGTCAGTTCCTACTGACTGTCACGTGGCAGAGTCCAGT CCAAAAGCTTGTTGATGAAAGAGTGGTTCANAACTCAGAATGTGGCCGGGCACATTGGCT CACACCTGTGATCCCAGCATTTTACGAGGCCGAGGCAGGAGGATCACTTGAGCCTGGGAG TTCAAGACCAGCCTGGGCAGCAGAACAAGACCCTGTCTCATTTTGAAAATAAATTAATAA GGCAAAAAAAAAAAAC
Restriction Sites	Please inquire
ACCN	BC033728
Insert Size	857 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC033728.1
RefSeq Size	857 bp
Locus ID	129685
Gene Summary	This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

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