ATPAF2 (BC032126) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ATP12; ATP12p; ATP synthase mitochondrial F1 complex assembly factor 2; LP3663; MGC29736
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC032126 edited GAGGTTTCCGTACAGCCCGACCGTGCGGCTTTGGGGATCCCATCGCTCTGTGTTTCCTGC TCCAGGTTTCGCGTCTCGCATCTTCCCAGACCCTCGGGCGCGATGTGGAGGAGCTGCCTC CGGCTGCGGGACGGGGGACGCCGTCTCCTGAATCGGCCGGCGGGTGGCCCCAGCGCTTCT ATGAGTCCGGGGCCAACCATCCCGTCTCCAGCCCGGGCTTACGCCCCGCCGACAGAAAGG AAGAGGTTTTATCAGAATGTCAGCATCACACAGGGTGAAGGTGGCTTTGAGATAAACCTG GACCACAGGAAGCTGAAAACTCCCCAAGCCAAGCTCTTTACCGTCCCCAGCGAGGCCCTG GCCATTGCAGTGGCTACTGAGTGGGATTCCCAGCAGGATACCATCAAGTACTACACCATG CACCTGACCACATTGTGCAACACATCATTGGACAACCCAACCCAGAGAAACAAGGATCAG CTGATCCGGGCAGCCGTGAAGTTTCTGGACACCGACACCATCTGCTACAGGGTGGAGGAG CCCGAGACATTAGTGGAACTTCAAAGGAATGAGTGGGATCCAATCATCGAATGGGCTGAG AAAAGATACGGCGTGGAGATCAGTTCCTCCACCAGCATAATGGGACCCAGCATCCCTGCC AAAACTCGGGAGGTGCTCGTCAGCCACCTGGCATCTTACAACACATGGGCTTTACAAGGG ATTGAGTTTGTAGCTGCCCAGCTCAAGTCCATGGTGCTAACCTTGGGCCTGATTGACCTG CGCCTGACAGTGGAGCAGGCCGTGCTGCTGTCACGCCTGGAGGAGGAGTACCAGATCCAG AAGTGGGGCAACATTGAGTGGGCCCATGACTATGAGCTGCAGGAGCTGCGGGCCCGCACC GCCGCCGGCACCCTCTTCATCCATCTCTGCTCCGAGAGCACCACAGTCAAGCACAAGCTC CTGAAGGAGTGAGGCCTGGGCAGAGCACACTCAGCAGGATAGAGGCAGTGCAGCCACAGC TCCCCCGGCCTTCAGGGCTCCCCAGCCTGTGGGGCTGGCTTCCTTGGCTTTTGGGGACTC GGCCTCAGCGTCACCCTGAGATTCCCCCCGAGACACAGTGCGCTAGTACGGCTGTCCGGA GGTCAGCCTGATTTCAACCCAGGTGCCCCTGGCCTGGCCAGCAGTGAATGTAGGAGATGA ATTGTGCAAGTGACTTTCTCTCGACTCTGATTTTATTAAATATTTCTCCACCCTGGAAAA AAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC032126 unedited NTTTTATTTATAATTGTATACGACTCACCATAGGCGGCACGCGAATTCGCACGAGGGAGG TTTCCGTACAGCCCGACCGTGCGGCTTTGGGGATCCCATCGCTCTGTGTTTCCTGCTCCA GGTTTCGCGTCTCGCATCTTCCCAGACCCTCGGGCGCGATGTGGAGGAGCTGCCTCCGGC TGCGGGACGGGGGACGCCGTCTCCTGAATCGGCCGGCGGGTGGCCCCAGCGCTTCTATGA GTCCGGGGCCAACCATCCCGTCTCCAGCCCGGGCTTACGCCCCGCCGACAGAAAGGAAGA GGTTTTATCAGAATGTCAGCATCACACAGGGTGAAGGTGGCTTTGAGATAAACCTGGACC ACAGGAAGCTGAAAACTCCCCAAGCCAAGCTCTTTACCGTCCCCAGCGAGGCCCTGGCCA TTGCAGTGGCTACTGAGTGGGATTCCCAGCAGGATACCATCAAGTACTACACCATGCACC TGACCACATTGTGCAACACATCATTGGACAACCCAACCCAGAGAAACAAGGATCAGCTGA TCCGGGCAGCCGTGAAGTTTCTGGACACCGACACCATCTGCTACAGGGTGGAGGAGCCCG AGACATTAGTGGAACTTCAAAGGAATGAGTGGGATCCAATCATCGAATGGGCTGAGAAAA GATACGGCGTGGAGATCAGTTCCTCCACCAGCATAATGGGACCCAGCATCCCTGCCAAAA CTCGGGAGGTGCTCGTCAGCCACCTGGCATCTTACAACACATGGGCTTTACAAGGGATTG AGTTTGTAGCTGCCCAGCTCAAGTCCATGGTGCTAACCTTGGGCCTGATTGACCTGCGCC TGACAGTGGAGCAGGCCGTGCTGCTGTCACGCCTGGAGGGAGAT
Restriction Sites	Please inquire
ACCN	BC032126
Insert Size	1290 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC032126.2, AAH32126.1
RefSeq Size	1281 bp
Locus ID	91647
Gene Summary	This gene encodes an assembly factor for the F(1) component of the mitochondrial ATP synthase. This protein binds specifically to the F1 alpha subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. This gene is located within the Smith-Magenis syndrome region on chromosome 17. An alternatively spliced transcript variant has been described, but its biological validity has not been determined. [provided by RefSeq, Jul 2008]

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