DNAJB8 (BC029521) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DJ6; DnaJ (Hsp40) homolog, subfamily B, member 8; DnaJ homolog, subfamily B, member 8; MGC33884
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC029521 edited GGCTGGCCCCACAGCGGCAGTGTCCCTCCCCTCCCCCCACTCCTCTCAGTGGGGGCCCCT CCAGTCCCTGAGAATTGGTACTACGAAAAGGTGAACTCCTGGGCAGAATCTTGCCTAGAG CTTGCGGAGTCCAGCCAGGCCCCTGCTGAAGGGCCCCAGACCACCGGCCACTTCTCCCCC GTCCATCTGACCAGCTGGGCCCCTGCGCCCACCTGGCCTCCACGTTCCCTCTCCTCTCAC CCACACCCCTGGCCATGGCTAACTACTACGAAGTGCTGGGCGTGCAGGCCAGCGCTTCCC CGGAGGACATCAAGAAAGCCTACCGCAAGCTGGCCCTTCGTTGGCACCCCGACAAGAACC CTGACAATAAGGAGGAGGCGGAGAAGAAGTTCAAGCTGGTGTCTGAGGCCTATGAGGTTC TGTCTGACTCCAAGAAACGCTCCCTGTATGACCGTGCTGGCTGTGACAGCTGGCGGGCTG GTGGCGGGGCCAGCACGCCCTACCACAGCCCCTTCGACACCGGCTACACCTTCCGTAACC CTGAGGACATCTTCCGGGAGTTTTTCGGTGGCCTGGACCCTTTCTCCTTTGAGTTCTGGG ACAGCCCATTCAATAGTGACCGTGGTGGCCGGGGCCATGGCCTGAGGGGGGCCTTCTCGG CAGGCTTTGGAGAATTTCCGGCCTTCATGGAGGCCTTCTCATCCTTCAACATGCTGGGCT GCAGCGGGGGCAGCCACACCACCTTCTCATCCACCTCCTTCGGGGGCTCCAGTTCTGGCA GCTCGGGGTTCAAGTCGGTGATGTCGTCCACCGAGATGATCAATGGCCACAAGGTCACCA CCAAGCGCATCGTGGAGAACGGGCAGGAGCGCGTGGAGGTGGAGGAAGACGGGCAGCTCA AGTCGGTGACTGTGAACGGCAAGGAGCAGCTCAAATGGATGGACAGCAAGTAGGCGCTGG CCACCCGGCCCTGCCTTCCCACCACCACCACCGTGCATGGGGCAGCAAACACGTGGGGCC GCAGACATAGCCTGATGGTTAATAAATGTGCCAAGTGAGTTCATGACAAAAAAAAAAAAA AA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC029521 unedited NAAGCGAATCGTATTTGTAAACGACTCATATAGGCGGCCGCACTAACTTCTATAGCATAC ATTATATCTAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGGG CTGGCCCCACAGCGGCAGTGTCCCTCCCCTCCCCCCACTCCTCTCAGTGGGGGCCCCTCC AGTCCCTGAGAATTGGTACTACGAAAAGGTGAACTCCTGTGCAGAATCTTGCCTAGAGCT TGCGAAGTCCAGCCAGGCCCCTGCTGAAGGGCCCCAGACCACCGGCCACTTCTCCCCCGT CCATCTGACCAGCTGGGCCCCTGCGCCCACCTGGCCTCCACGTTCCCTCTCCTCTCACCC ACACCCCTGGCCATGGCTAACTACTACGAAGTGCTGGGCGTGCAGGCCAGCGCTTCCCCG GAGGACATAAAGAAAGCCTACCGCAAGCTGGCCCTTCGTTGGCACCCCGACAAGAACCCT GACAATAAGGAGGAGGCGGAGAAGAAGTTCAAGCTGGTGTCTGAGGCCTATGAGGTTCTG TCTGACTCCAAGAAACGCTCCCTGTATGACCGTGCTGGCTGTGACAGCTGGCGGGCTGGT GGCGGNGCCAGCACGCCCTACCACAGCCCCTTCGACACCCGCTACACCTTCCGTAACCCT GAGGACATCTTCCGGGGATTTTTCGGTGGCCTGGACCCTTTCTCCTTTGAGTTCTGGACA GCCCATTCATAATGACCGAGGTGACCCGGGGCCATGGCCTGAAGGGGGCCTTCTCGGAGG CTTTGGGAAATTTCGGCCCTTATGGAGGCCTTTTAACCTTCAACATGCTGGGCTGCAACG GGGGCAGACACACCACCTTTTAATCCACCTTCTATCGGGGGCT
Restriction Sites	Please inquire
ACCN	BC029521
Insert Size	1082 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC029521.1, AAH29521.1
RefSeq Size	1082 bp
Locus ID	165721
Gene Summary	The protein encoded by this gene belongs to the DNAJ/HSP40 family of proteins that regulate chaperone activity. This family member suppresses aggregation and toxicity of polyglutamine proteins, and the C-terminal tail is essential for this activity. It has been implicated as a cancer-testis antigen and as a cancer stem-like cell antigen involved in renal cell carcinoma. [provided by RefSeq, Jun 2012]

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