CLASP2 (BC029035) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CLIP-associating protein 2; cytoplasmic linker associated protein 2; multiple asters (Mast)-like homolog 2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC029035 edited GCCGGCACAAACCTCAGTGGTGGTTCTGTGGTTGTTTCTGTCTTTTTTTGATACAATCTT TGATTAGTATCGAATTTACTGTATTTGGCCATGTGAACTATTGGGAGCCTCCTAGGGTGA GGGAAATTAAGAGCTTTCAGAGGAATGAGGCGACTGATTTGCAAACGGATCTGTGATTAT AAAAGCTTCGATGATGAAGAATCAGTGGATGGAAATAGGCCATCATCAGCTGCATCAGCC TTCAAGGTTCCTGCACCTAAAACATCCGGAAATCCTGCCAACAGTGCAAGGAAGCCTGGT TCAGCAGGTGGCCCTAAGGTTGGAGGTGCTTCTAAGGAAGGAGGTGCTGGAGCAGTTGAT GAAGATGATTTTATAAAAGCTTTTACAGATGTCCCTTCTATTCAGATTTATTCTAGTCGA GAACTCGAAGAAACATTAAATAAAATCAGGGAAATTTTGTCAGATGATAAACATGACTGG GATCAGCGTGCCAATGCACTGAAGAAAATTCGATCACTGCTTGTTGCTGGAGCTGCACAG TATGATTGCTTTTTTCAACATTTACGATTGTTGGATGGAGCACTTAAACTTTCAGCTAAG GATCTTAGATCCCAGGTGGTTAGAGAAGCTTGTATTACTGTAGCCCACCTTTCAACAGTT TTGGGAAACAAGTTTGATCATGGCGCTGAAGCCATTGTACCTACACTTTTTAATCTCGTC CCCAATAGTGCAAAAGTCATGGCAACTTCTGGATGTGCAGCAATCAGATTTATCATTCGG CATACTCATGTACCCAGACTTATACCTTTAATAACAAGCAATTGCACATCAAAATCAGTT CCCGTGAGGAGACGTTCATTTGAATTTTTAGATTTATTGTTGCAAGAGTGGCAGACTCAT TCATTGGAAAGACATGCAGCCGTCTTGGTTGAAACTATTAAAAAGGGAATTCATGATGCT GACGCTGAGGCCAGAGTGGAGGCAAGAAAGACATACATGGGTCTTAGAAACCACTTTCCT GGTGAAGCTGAAACATTATATAATTCCCTTGAGCCATCTTATCAGAAGAGTCTTCAAACT TACTTAAAGAGTTCTGGCAGTGTAGCATCTCTTCCACAATCAGACAGGTCCTCATCCAGC TCACAGGAAAGTCTCAATCGCCCTTTTTCTTCCAAATGGTCTACAGCAAATCCATCAACT GTGGCTGGAAGAGTATCAGCAGGCAGCAGCAAAGCCAGTTCCCTTCCAGGAAGCCTGCAG CGTTCACGAAGTGACATTGATGTGAATGCTGCTGCAGGTGCCAAGGCACATCATGCTGCT GGACAGTCTGTGCGAAGCGGGCGCTTAGGTGCAGGTGCCCTGAATGCAGGTTCCTATGCG TCACTAGAATGTGAAGCCTTTTGGAGATCTGGGAGAACTGCAAAGCTCTACTCTGTCTAA CCAAACCCTAAACATATATTCCAAAACTCTATTCAGTTTGATTTCTTCATGTTTTTATGT CTATCAAATTAAAAAGTTGACGTTGGTTTGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC029035 unedited NNNAAGTTCCATACTTTGTTAATACCGATTCACTATTAGGGCGGCCCGCGNATATCATAT CTGGTACCGGTCCCGGATTTCCCGGGCTGCCGGCACAAACCTCAGTGGTGGTTCTGTGGT TGTTTCTGTCTTTTTTTGATACAATCTTTGATTAGTATCGAATTTACTGTATTTGGCCAT GTGAACTATTGGGAGCCTCCTAGGGTGAGGGAAATTAAGAACTTTCAGAGGAATTGAGGC GACTGATTTGCAAACGGATCTGTGATTATTAAAGCTTCGATTGATGAAGAATCAGTGGAT GGAAATAGGCCATCATCAGCTGCATCAGCCTTCAAGGTTCCTGCACCTAAAACATCCGGA AATCCTGCCAACAGTGCAAGGAAGCCTGGTTCAGCAGGTGGCCCTAAAGTTGGAGGTGCT TCTAAAGAAGGAGGTGCTGGAGCAGTTGATGAAGATGATTTTATAAAAGCTTTTACAGAT GTCCCTTCTATTCAGATTTATTCTAGTCGAGAACTCGAAGAAACATTAAATAAAATCAGG GAAATTTTGTCAGATGATAAACATGACTGGGATCAGCGTGCCAATGCACTGAAGAAAATT CGATCACTGCTTGTTGCTGGAGCTGCACAGTATGATTGCTTTTTTCAACATTTACGATTG TTGGATGGAGCACTTAAACTTTCAGCTAAAGATCTTAGATCCCAGGTGGTTAGAGAAGCT TGTATTACTGTAGCCCACCTTTCAACAGTTTTAGGAAACAAGTTTGATCATGGCGCTGAA GCCATTGTACCTACACTTTTTAATCTCGTCCNCNATAGTGCAAAAGTTCATGGCACTTCT GGATGTGCAGCANTCAGATTTATCATTTCGCATACTCATGTACCCAGACTTATACCTTTT ATNAANCAGCATTGCACATCAAAATCAGTTTCCGTGAGGAGACGTTCATTTTGAT
Restriction Sites	Please inquire
ACCN	BC029035
Insert Size	1583 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC029035.1, AAH29035.1
RefSeq Size	1583 bp
Locus ID	23122
Gene Summary	Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules (PubMed:26003921). Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2 (PubMed:16824950). This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (PubMed:16866869, PubMed:16914514). Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.[UniProtKB/Swiss-Prot Function]

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