DDI1 (BC022017) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DDI1, DNA-damage inducible 1, homolog 1; DDI1, DNA-damage inducible 1, homolog 1 (S. cerevisiae); DNA-damage inducible protein 1; FLJ36017
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC022017 edited AAGGCTCCACCTGGCCACACAGCAGGCACTGAGGCTGGGAGACAGCCCCCAGACAGATGA GTGTCCGCGCCTCTCTGAGAGGTGAATGAGCCCGGACGGTCCCTACCTACCAAGTCCTGA GGAGCAGCGGCACCAACGACGCAGGCCCGCCCCAGCCCGCCAGTGAGCCGCCCATGCCCT CTGCTAGCCCGGCCCGCCCGGGCCCCCGCCATGCTGATCACCGTGTACTGCGTGCGGAGG GACCTCTCCGAGGTCACCTTCTCTCTCCAGGTCAGCCCCGACTTTGAGCTCCGAAACTTC AAGGTCCTCTGCGAAGCGGAGTCCAGAGTCCCCGTCGAAGAGATCCAGATCATCCACATG GAGCGACTCCTCATCGAGGACCACTGTTCCCTGGGCTCCTACGGCCTCAAAGATGGCGAT ATCGTGGTTTTACTGCAGAAGGACAATGTGGGACCTCGGGCTCCAGGGCGTGCCCCGAAC CAGCCTCGTGTAGACTTCAGTGGCATTGCGGTGCCTGGGACGTCCAGCTCCCGTCCACAG CACCCTGGACAGCAGCAGCAGCGCACACCCGCTGCCCAGCGGTCACAGGGCTTGGCGTCA GGAGAGAAGGTGGCCGGCCTGCAAGGTCTGGGCAGCCCCGCCCTGATCCGCAGCATGCTG CTCTCCAACCCCCACGATCTGTCCCTGCTCAAGGAACGCAACCCTCCCTTGGCGGAAGCC CTGCTCAGCGGAAGCCTTGAGACCTTTTCTCAGGTGCTGATGGAGCAGCAAAGGGAAAAG GCCTTGAGAGAGCAAGAGAGGCTTCGTCTCTACACAGCCGACCCACTGGATCGGGAAGCT CAGGCCAAAATAGAAGAGGAAATCCGGCAGCAAAACATTGAAGAAAACATGAATATAGCG ATAGAAGAGGCCCCCGAGAGTTTTGGACAAGTGACGATGCTCTACATTAACTGCAAAGTG AATGGGCATCCTTTGAAGGCTTTTGTTGACTCGGGCGCCCAGATGACCATTATGAGCCAG GCTTGTGCCGAGCGATGTAACATCATGAGGCTGGTGGACCGACGGTGGGCTGGGGTTGCT AAAGGAGTGGGCACACAGAGAATTATTGGCCGTGTTCATCTAGCTCAGATTCAAATTGAA GGTGATTTCTTACAGTGCTCTTTCTCCATACTTGAGGATCAACCCATGGATATGCTTCTA GGCCTAGATATGCTCCGGAGACATCAATGTTCCATCGATTTGAAGAAAAATGTGCTGGTC ATCGGCACCACTGGCACGCAGACTTATTTTCTTCCTGAGGGAGAGTTGCCCTTATGCTCT AGGATGGTAAGTGGGCAAGATGAGTCTTCGGACAAGGAAATTACACATTCAGTCATGGAT TCAGGACGAAAAGAACATTAAAGCACGTTATAAATATGTTACCACCTTGAGGGAGCCTCA GGTCCCCGGCAATTATAAGTTAAGAGCTTACTGGCAATGTAATCATTAAAAAACATCAGT AACAACTAAACCTGGCCTTGGGACTACGTTCTCAGATGGGTAACTAACGTCAATCCTGAT TCCTTGGTCTTGGTCCCTCTTCCATTTCCCTTGTCCAGAGATCACTGAAAGGCATCCTGG GAAGGCTTTGGAGGCTCCAGCTGATTTCCAGATAATCCATGAAAAAGAAAACCAGCTTCT TCCTTTAGAGACACTATCCTATCAGATTACAGGTGGCTTATCCAAATCATTGCTATTTTG CAGAATTCCCTGGAAAAATGCTATGGCTGCAGACCTTACAAGGCATCCACATAAAACTAT TTCACATCAGCACTAATAATTCTTTCTACTGAGAGAAATATGCCCATTTTCACAGGTTTT GTTTTCTTTGGGAAGGGTATTAGAAATGGTAGGTCAGTTACACTATGATGTTAACTAATA CTTATACATGTCTGTTTGGGGCAGCATAAAGACCAATTTCCTCATATTTACACCTTCCCT TCCCCTGGATCCCCTGCAGTCACCCTGCAATCCACAGCTAAAGACTATTTTTGCCATATT TGAGAAAGTGGATTTATCTCCAAGGATGCTACATTAATTTTCTATTTCTCCTGCATTCAA AGAAACTCTGGCTATAAAACAAAGGCAATAAATTAGATTTTTTAAAAAAAGAAAAAAAAA AAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC022017 unedited CTTGGTTTTGTAATACGACGTCTGATAGGCGGCCGCATAACTTCGTATAGGATACATTAT ATTTTGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGAAGGCTCC ACCTGGCCACACAGCAGGCACTGAGGCTGGGAGACAGCCCCCAGACAGATGAGTGTCCGC GCCATTCTGAGAGGTGAATGAGCCCGGACGGTCCCTACCTACCAAGTCCTGAGGAGCAGC GGCACCAACGACGCATGCCCGCCCCAGCCCGCCAGTGAGCCGCCCATGCCCTCTGCTAGC CCGGCCCGCCCGGGCCCCCGCCATGCTGATCACCGTGTACTGCGTGCGGAGGGACCTCTC CGAGGTCACCTTCTCTCTCCAGGTCAGCCCCGACTTTGAGCTCCGAAACTTCAAGGTCCT CTGCGAAGCGGAGTCCAGAGTCCCCGTCGAAGAGATCCAGATCATCCACATGGAGCGACT CCTCATCGAGGACCACTGTTCCCTGAGCTCCTACGGCCTCAAAGATGGCGATATCGTGGT TTTACTGCAGAAGGACAATGTGGGACCTCGGGCTCCAGGGCGTGCCCCGAACCAGCCTCG TGTAGACTTCAGTGGCATTGCGGTGCCTGGGACGTCCAGCTCCCGTCCACAGCACCCTGG ACAGCAGCAGCAGCGCACACCCGCTGCCCAGCGGTCACAGGGCTTGGCGTCAGGAGAGAA TGTGGCCCGCCTGCAAAGTCTGGGCAGCCCCGCCCTGATCCGCANCATGCTGCTCNTCAC CCCCACGATCTGTCCCTGCTCAGGGACGCACCCNTCCCTGGNCGNAGCCCTGCTCANCGG AAGCTTGAGACCTTTCTCAGTGCTGATGGACANCAAAGGGAAATGCCTTGANAGAGCAGA GAGGCTCGTCTCTCACAGCCGACCCACTGGTCGG
Restriction Sites	Please inquire
ACCN	BC022017
Insert Size	2171 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC022017.2, AAH22017.1
RefSeq Size	2166 bp
Locus ID	414301
Gene Summary	Probable aspartic protease (Probable). Seems to act as a proteasomal shuttle which links the proteasome and replication fork proteins like RTF2 (Probable). Required, with DDI2, for cellular survival following replication stress. Together or redudantly with DDI2, removes RTF2 from stalled forks to allow cell cycle progression after replication stress and maintains genome integrity (PubMed:29290612).[UniProtKB/Swiss-Prot Function]

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