C19orf61 (SMG9) (BC008869) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C19orf61; F17127_1; HBMS
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC008869 edited ATCTCAGCTCGGGCCCGCGTTACATCCTCCTCCTCTTCTTCCTTCGGCCCAGCTTTCCTT AGGGGCTGCAACCCGGACGCCGAGGCCGGTTTCGGAGTGGGGAGTGCCCATTTTCTCTCC TTCCCACGTTCCTGGCCCCCAGACGCCATTTGCAGGCGGGTGGCTTGGGTCAGCCTCCCC GCCCCCACCCGACTCCCGTCACGGGAGAGCGCACACCGCGCCCCGAGAACCAATCAGCAG CCGCGTTAGGTAACCATGTCTGAGTCTGGACACAGTCAGCCTGGACTCTATGGGATAGAG CGGCGGCGACGGTGGAAGGAGCCTGGCTCTGGTGGCCCCCAGAATCTCTCTGGGCCTGGT GGTCGGGAGAGGGACTACATTGCACCATGGGAAAGAGAGAGAAGGGATGCCAGCGAAGAG ACAAGCACTTCCGTCATGCAGAAAACCCCCATCATCCTCTCAAAACCTCCAGCAGAGCGG TCAAAACAGCCACCACCTCCAACAGCCCCTGCTGCCCCGCCTGCTCCAGCCCCTCTGGAG AAGCCCATCGTTCTCATGAAGCCACGGGAGGAGGGGAAGGGGCCTGTGGCCGTGACAGGT GCCTCTACCCCTGAGGGCACCGCCCCACCACCCCCTGCAGCCCCTGCGCCACCCAAGGGG GAGAAGGAGGGGCAGAGACCCACACAGCCTGTGTACCAGATCCAGAACCGGGGCATGGGC ACTGCCGCACCAGCAGCCATGGACCCTGTCGTGGGTCAGGCCAAACTACTGCCCCCAGAG CGCATGAAGCACAGCATCAAGTTGGTGGATGACCAGATGAATTGGTGTGACAGTGCCATC GAGTACCTGTTGGATCAGACTGATGTGTTGGTGGTTGGTGTCCTGGGCCTCCAGGGGACA GGCAAGTCCATGGTCATGTCATTGTTGTCAGCCAACACTCCAGAGGAGGACCAGAGGACT TATGTTTTCCGGGCCCAGAGCGCTGAAATGAAGGAACGAGGGGGCAACCAGACCAGTGGC ATCGACTTCTTTATTACCCAAGAACGGATTGTTTTCCTGGACACACAGCCCATCCTGAGC CCTTCTATCCTAGACCATCTCATCAATAATGACCGCAAACTGCCTCCAGAGTACAACCTT CCCCACACTTACGTTGAAATGCAGTCACTCCAGATTGCTGCCTTCCTTTTCACGGTCTGC CATGTGGTGATTGTTGTCCAGGACTGGTTCACAGACCTCAGTCTCTACAGGTTCCTGCAG ACAGCAGAGATGGTGAAGCCCTCCACCCCATCCCCCAGCCACGAGTCCAGCAGCTCATCG GGCTCCGATGAAGGCACCGAGTACTACCCCCACCTAGTCTTCTTGCAGAACAAAGCTCGC CGAGAGGACTTCTGTCCTCGGAAGCTGCGGCAGATGCACCTGATGATTGACCAGCTCATG GCCCACTCCCACCTGCGTTACAAGGGAACTCTGTCCATGTTACAATGCAATGTCTTCCCG GGGCTTCCACCTGACTTCCTGGACTCTGAGGTCAACTTATTCCTGGTACCCTTCATGGAC AGTGAAGCAGAGAGTGAAAACCCACCAAGAGCAGGACCTGGTTCCAGCCCACTCTTCTCC CTGCTGCCTGGGTATCGTGGCCACCCCAGTTTCCAGTCCTTGGTGAGCAAGCTCCGGAGC CAAGTGATGTCCATGGCCCGGCCACAGCTGTCACACACGATCCTCACCGAGAAGAACTGG TTCCACTACGCTGCCCGGATCTGGGATGGGGTGAGAAAGTCCTCTGCTCTGGCAGAGTAC AGCCGCCTGCTGGCCTGAGGCCAAGGAGAGGAATGTCATGCAGGGGACCTCCTGGGTCCG CAGTGTACTGCGAGGGAGCACAGATGTCCATCCCCCGCTGGGGTGGAGAGCGGCAGCAGG CCTGATGGATGAGGGATCGTGGCTTCCCGGCCCAGAGACATGAGGTGTCCAGGGCCAGGC CCCCCACCCTCAGTTGGGGCTGTTCCGGGGGTGACTGTGAGCGATCCCACCCCAAACCTG AGATGGGGTAGCCCGTCCTGTGTCCTCCACAGGGACAAGCAGTGGGAGGAGTCTGAATGG TCACCAGGAAGCCCGGGCTCCATCTTGACCTCCTTTTTCAGGGACAGGAGCAACAGGCCC CTCTTCCCTGACTCTAAGCCCTTCCCTGTAAGGTGAGGCAGGGTCTGGAGAGCTCTTTAT TGGAACAGATCTGGTGGTTCAAATAAACACAGTCATGCAAAAAAAAAAAAAAAAAAAAAA AA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC008869 unedited NAAGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGATCTCAG CTCGGGCCCGCGTTACATCCTCCTCCTCTTCTTCCTTCGGCCCATCTTTCCTTAGGGGCT GCAACCCGGACGCCGAGGCCGGTTTCGGAGTGGGGAGTGCCCATTTTCTCTCCTTCCCAC GTTCCTGGCCCCCAGACGCCATTTGCAGGCGGGTGGCTTGGGTCAGCCTCCCCGCCCCCA CCCGACTCCCGTCACGGGAGAGCGCACACCGCGCCCCGAGAACCAATCAGCAGCCGCGTT AGGTAACCATGTCTGAGTCTGGACACAGTCAGCCTGGACTCTATGGGATAGAGCGGCGGC GACGGTGGAAGGAGCCTGGCTCTGGTGGCCCCCAGAATCTCTCTGGGCCTGGTGGTCGGG AGAGGGACTACATTGCACCATGGGAAAGAGAGAGAAGGGATGCCAGCGAAGAGACAAGCA CTTCCGTCATGCAGAAAACCCCCATCATCCTCTCAAAACCTCCAGCAGAGCGGTCAAAAC AGCCACCACCTCCAACAGCCCCTGCTGCCCCGCCTGCTCCAGCCCCTCTGGAGAAGCCCA TCGTTCTCATGAAGCCACGGGAGGAGGGGAAGGGGCCTGTGGCCGTGACAGGTGCCTCTA CCCCTGAGGGCACCGCCCCACCACCCCCTGCAGCCCCTGCGCCACCCAAGGGGGATGAGG AGGGGCAGAGACCCACACAGCCTGTGTACCAGATCCAGAACCGGGGCATGGGCACTGCCG CACCAGCAGCCATGGACCCTGTCGTGGGTCATGCCAAACTACTGCCCCCAGAGCGCATGA AGCACAGCATCAAGTTGGTGGATGACCAGATGAATTGGNTGTGACAGTGCCATCGAGTAC CTGTTT
Restriction Sites	Please inquire
ACCN	BC008869
Insert Size	2291 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC008869.2, AAH08869.1
RefSeq Size	2282 bp
Locus ID	56006
Gene Summary	This gene encodes a regulatory subunit of the SMG1 complex, which plays a critical role in nonsense-mediated mRNA decay (NMD). Binding of the encoded protein to the SMG1 complex kinase scaffold protein results in the inhibition of its kinase activity. Mutations in this gene cause a multiple congenital anomaly syndrome in human patients, characterized by brain malformation, congenital heart disease and other features. [provided by RefSeq, Jul 2016]

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