CHRM1 (BC007740) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	cholinergic receptor, muscarinic 1; HM1; M1; MGC30125; muscarinic acetylcholine receptor M1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC007740 edited GGCAGTGGGGTTGAGGACCCTACAGACCCCTCTTCAGCCCCGTGGTGATGACTTTCCCCT GAGGAAGCCCTGTAGCGTGCCTGGAGGAAGGGGCTCTCCAACCCCAGCCCCACCTAGCCA CCATGAACACTTCAGCCCCACCTGCTGTCAGCCCCAACATCACCGTCCTGGCACCAGGAA AGGGTCCCTGGCAAGTGGCCTTCATTGGGATCACCACGGGCCTCCTGTCGCTAGCCACAG TGACAGGCAACCTGCTGGTACTCATCTCTTTCAAGGTCAACACGGAGCTCAAGACAGTCA ATAACTACTTCCTGCTGAGCCTGGCCTGTGCTGACCTCATCATCGGTACCTTCTCCATGA ACCTCTATACCACGTACCTGCTCATGGGCCACTGGGCTCTGGGCACGCTGGCTTGTGACC TCTGGCTGGCCCTGGACTATGTGGCCAGCAATGCCTCCGTCATGAATCTGCTGCTCATCA GCTTTGACCGCTACTTCTCCGTGACTCGGCCCCTGAGCTACCGTGCCAAGCGCACACCCC GCCGGGCAGCTCTGATGATCGGCCTGGCCTGGCTGGTTTCCTTTGTGCTCTGGGCCCCAG CCATCCTCTTCTGGCAGTACCTGGTAGGGGAGCGGACAGTGCTAGCTGGGCAGTGCTACA TCCAGTTCCTCTCCCAGCCCATCATCACCTTTGGCACAGCCATGGCTGCCTTCTACCTCC CTGTCACAGTCATGTGCACGCTCTACTGGCGCATCTACCGGGAGACAGAGAACCGAGCAC GGGAGCTGGCAGCCCTTCAGGGCTCCGAGACGCCAGGCAAAGGGGGTGGCAGCAGCAGCA GCTCAGAGAGGTCTCAGCCAGGGGCTGAGGGCTCACCAGAGACTCCTCCAGGCCGCTGCT GTCGCTGCTGCCGGGCCCCCAGGCTGCTGCAGGCCTACAGCTGGAAGGAAGAAGAGGAAG AGGACGAAGGCTCCATGGAGTCCCTCACATCCTCAGAGGGAGAGGAGCCTGGCTCCGAAG TGGTGATCAAGATGCCAATGGTGGACCCCGAGGCACAGGCCCCCACCAAGCAGCCCCCAC GGAGCTCCCCAAATACAGTCAAGAGGCCGACTAAGAAAGGGCGTGATCGAGCTGGCAAGG GCCAGAAGCCCCGTGGAAAGGAGCAGCTGGCCAAGCGGAAGACCTTCTCGCTGGTCAAGG AGAAGAAGGCGGCTCGGACCCTGAGTGCCATCCTCCTGGCCTTCATCCTCACCTGGACAC CGTACAACATCATGGTGCTGGTGTCCACCTTCTGCAAGGACTGTGTTCCCGAGACCCTGT GGGAGCTGGGCTACTGGCTGTGCTACGTCAACAGCACCATCAACCCCATGTGCTACGCAC TCTGCAACAAAGCCTTCCGGGACACCTTTCGCCTGCTGCTGCTTTGCCGCTGGGACAAGA GACGCTGGCGCAAGATCCCCAAGCGCCCTGGCTCCGTGCACCGCACTCCCTCCCGCCAAT GCTGATAGTCCCCTCTCCTGCATCCCTCCACCCCAGTCCCCGGGAAAGGCCGGTGGGAAG AGGGCAGGGGCTGCATCCTCAGCCCCAGGGCCCTGCTCAGGCCTCACCTGGCTTCCCAGG ACCCTGGGTCACCTTCCTGGGCAGCCCAGAGAGACCCTGCCAACTTTCCAGACTTCGCTA TTCCCAGGCAGGGAGGGAAACCCGGGGAACTGGTTTTTCTGTTCCCTGCTGGGTGGGAAT GCGCTCTTCACCAGGAAGAAGGCCCGGGAGGAGGATCCGGGCTTTGGACTCCTTGTTTGC CTTTAGGCAGGAAGTCAGGAGCCAGCAGGGCGGGCCAGGAGAAAGAAGGCTTAACATTAA GTATTCCTTGGCCCAGCAGCGGCCCAGATTGCGGTGTGAGATGGTGCCCCCTGGGGGGCA CAGCCAGAAACTGAACTGGCCGCTGGGAGAAAAGCCAGATGACAGGGAGCTGGGGAATCC CCTCGCTTCATAGGCAGAGCCCGCCCACCTGGGCCCTAGGCATACTCTCCAGGATTGTCC ACAAATGTCCTCAGAGGGTCCCTAGGTGGGTCAACTCCAAGGCAAATGTCCAAGCATCAG CAAGACAATGACACTGGAAGGGTCCGGCTTGGCTAGTCACATATCAAGTCCCGAGGCAGC AACAGGACCAGGAGCCAGGTGTCCTGACTGTCCTACAATATCATTTTCCTGGGAGTGGGA GTCAAGTGTGCCTGCTATCCAGCCGCAAATCCATACCCCCTGCCCCAGAGAAGCCTCAGT CCCTCCCTCCTGGCTCACAGCCACCACCTGGATGGATCTGCTCCATGCAGATCTAGCCAG GCCTCCCGCATGCTGCCTGCCTCCGGCCCTGCCCCACACAGGCCTGGCCCAGCCAGCAGG TTCTCTCCTGTGAGCTCCCCAATCCAACCCATGCATGGCCTCCCAGCCACCCGGATCTCC AGGCCCAGCCTGGCCCCAAATGTTCTTTCCTTTCATCCTCAGCAAGTGCTGAGTCTGTGA ATAAAGCCACATAACCAGCGGGCAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC007740 unedited NGAGTCATCTTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGCAGTG GGGTTGAGGACCCTACATACCCCTCTTCAGCCCCGTGGTGATGACTTTCCCCTGAGGAAG CCCTGTAGCGTGCCTGGAGGAAGGGGCTCTCCAACCCCAGCCCCACCTAGCCACCATGAA CACTTCAGCCCCACCTGCTGTCAGCCCCAACATCACCGTCCTGGCACCAGGAAAGGGTCC CTGGCAAGTGGCCTTCATTGGGATCACCACGGGCCTCCTGTCGCTAGCCACAGTGACAGG CAACCTGCTGGTACTCATCTCTTTCAAGGTCAACACGGAGCTCAAGACAGTCAATAACTA CTTCCTGCTGAGCCTGGCCTGTGCTGACCTCATCATCGGTACCTTCTCCATGAACCTCTA TACCACGTACCTGCTCATGGGCCACTGGGCTCTGGGCACGCTGGCTTGTGACCTCTGGCT GGCCCTGGACTATGTGGCCAGCAATGCCTCCGTCATGAATCTGCTGCTCATCAGCTTTGA CCGCTACTTCTCCGTGACTCGGCCCCTGAGCTACCGTGCCAAGCGCACACCCCGCCGGGC AGCTCTGATGATCGGCCTGGCCTGGCTGGTTTCCTTTGTGCTCTGGGCCCCAGCCATCCT CTTCTGGCAGTACCTGGTAGGGGAGCGGACAGTGCTAGCTGGGCAGTGCTACATCCAGTT CCTCTCCCAGCCCATCATCACCTTTGGCACAGCCATGGCTGCCTTCTACCTCCCTGTCAC AGTCATGTGCACGCTCTACTGGCGCATCTACCGGGAGACAGAGAACCGAGCACGGGAGCT GGCAGCCCTTCAGGGCTCCGAGACGCCAGGCTAGGGGGTGGCAGCAGCACG
Restriction Sites	Please inquire
ACCN	BC007740
Insert Size	2574 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC007740.2, AAH07740.1
RefSeq Size	2565 bp
RefSeq ORF	1380 bp
Locus ID	1128
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Calcium signaling pathway, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton
Gene Summary	The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.