MST1 (STK4) (BC005231) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	dJ211D12.2 (serine/threonine kinase 4 (MST1, KRS2)); DKFZp686A2068; kinase responsive to stress 2; KRS2; mammalian sterile 20-like 1; MST1; OTTHUMP00000043418; serine/threonine kinase 4; yeast Ste20-like; YSK3
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC005231 edited AGGTCCGCGGGAGGATGGAGCAGTGAGCGGGTCTGGGCGGCTGCTGGCAGCGCCATGGAG ACGGTACAGCTGAGGAACCCGCCGCGCCGGCAGCTGAAAAAGTTGGATGAAGATAGTTTA ACCAAACAACCAGAAGAAGTATTTGATGTCTTAGAGAAACTTGGAGAAGGGTGAGTGTAA AGAAACTATAGGTAGGTCATTGGGTCCCAGTCTTTTTCCTGCCCCAGAAGAAGCAGAAGG ATATGAACCTTTCAGCATTGTTCTAGGTGGGGTGGAAGGTAAATTTACAGCTTGTGATGT CCTTCTTCGCTTTACTCCAATCCCTATTATAGACAGATTTAGTGATTCCTGGTCTTTTTA ACACGAAGAATATCTATTGTTTTCTCTTTTGTAGGATCTGTATGATTTTATCTACTTAAC AGATAGCACTAATTAGATTAAAATTCTATAAGAAACTTTTTAATTTGCTGTTCATAATTT CTGATTGGTATGCAATAACTGTTTCAATGAAAATCAATGTAATTTAGTATTTTAATATTT GCACCTTTGTGAAATATAGTAAATAAATTAAGCACTATCACCACCTTCACAGCTACTTAG GAGATCCACAATCCTGGGTTGGGAGCCAGTGGATTTCCTGAAACACAGATTTGTTAATGC GTTTATAAAATACTGATGAAATGATATTAGTAATGTGCAGTGACCATCATTGACTTTCAT GATACAATTAAGTTTACATAATTTTTATACTTTTATATATCATTTTAATTCAATCCTGTG TATTTATTCCTTCTTCTGTAATGACCAGGAAATTAAAATGTTAATGACACCATTTCTTAA GTTTTTCTTGTTAATTTTTGAATAGGCGATACAATCATATGATTCAAAATTTAAAAAAAT ATTAAGACGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC005231 unedited NCCTGTTCGGATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCCATTACGGCCGG GGAGGTCCGCGGGAGGATGGAGCAGTGAGCGGGTCTGGGCGGCTGCTGGCAGCGCCATGG AGACGGTACAGCTGAGGAACCCGCCGCGCCGGCAGCTGAAAAAGTTGGATGAAGATAGTT TAACCAAACAACCAGAAGAAGTATTTGATGTCTTAGAGAAACTTGGAGAAGGGTGAGTGT AAAGAAACTATAGGTAGGTCATTGGGTCCCAGTCTTTTTCCTGCCCCAGAAGAAGCAGAA GGATATGAACCTTTCAGCATTGTTCTAGGTGGGGTGGAAGGTAAATTTACAGCTTGTGAT GTCCTTCTTCGCTTTACTCCAATCCCTATTATAGACAGATTTAGTGATTCCTGGTCTTTT TAACACGAAGAATATCTATTGTTTTCTCTTTTGTAGGATCTGTATGATTTTATCTACTTA ACAGATAGCACTAATTAGATTAAAATTCTATAAGAAACTTTTTAATTTGCTGTTCATAAT TTCTGATTGGTATGCAATAACTGTTTCAATGAAAATCAATGTAATTTAGTATTTTAATAT TTGCACCTTTGTGAAATATAGTAAATAAATTAAGCACTATCACCACCTTCACAGCTACTT AGGAGATCCACAATCCTGGGTTGGGAGCCAGTGGATTTCCTGANACACAGATTTGTTAAT GCGTTTATAAAATACTGATGAAATGATATTAGTAATGTGCAGTGACCATCATTGACTTTC ATGATACAATTAAGTTTACATAATTTTTATACTTTTATATATCATTTTAATTCAATCCTG TGTATTTATTCCTTNCTNCTGTAATGACCAGGAAATTAAAATGGTTATGACACCTT
Restriction Sites	Please inquire
ACCN	BC005231
Insert Size	942 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC005231.1, AAH05231.1
RefSeq Size	942 bp
Locus ID	6789
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	MAPK signaling pathway, Non-small cell lung cancer, Pathways in cancer
Gene Summary	The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq, Jul 2008]

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