MRPS21 (NM_031901) Human Untagged Clone
CAT＃: SC125003
MRPS21 (untagged)-Human mitochondrial ribosomal protein S21 (MRPS21), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 1200.00
CNY 2950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MDS016; MRP-S21; RPMS21 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC125003 sequence for NM_031901 edited (data generated by NextGen Sequencing)
ATGGCAAAACATCTGAAGTTCATCGCCAGGACTGTGATGGTACAGGAAGGGAACGTGGAA AGCGCATACAGGACCCTAAACAGAATCCTCACTATGGATGGGCTCATTGAGGACATTAAG CATCGGCGGTATTATGAGAAGCCATGCCGCCGGCGACAGAGGGAAAGCTATGAAAGGTGC CGGCGGATCTACAACATGGAAATGGCTCGCAAGATCAACTTCTTGATGCGAAAGAATCGG GCAGATCCGTGGCAGGGCTGCTGA Clone variation with respect to NM_031901.4 >OriGene 5' read for NM_031901 unedited
GGGGTCACAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGGTGGGCTA GGTACTGAGCGCGCGAGGCTCTACAGAGTGAAGGTTTAAATCCAAGGTCATGGCAAAACA TCTGAAGTTCATCGCCAGGACTGTGATGGTACAGGAAGGGAACGTGGAAAGCGCATACAG GACCCTAAACAGAATCCTCACTATGGATGGGCTCATTGAGGACATTAAGCATCGGCGGTA TTATGAGAAGCCATGCCGCCGGCGACAGAGGGAAAGCTATGAAAGGTGCCGGCGGATCTA CAACATGGAAATGGCTCGCAAGATCAACTTCTTGATGCGAAAGAATCGGGCAGATCCGTG GCAGGGCTGCTGAGGCCTGTGGGTGGGACACCCAGTGCGAAACCCTCATCCAGTTTTCTC TCCATCTCTTTTCTTTGTACAATCCCATTTCCTATTACCATTCTCTGCAATAAACTCAAA TCACATGTCTGCAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCAT AGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCT GGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCAT CATTTTGTCTGACTAGGTGTCCTTCTATATATTATGGGGTGGAGGGGGTGGTATGGAGCA AGGGGCAAGTTGGGAAGACACCTGTAGGCCCTGNCGGGTCTATTGGGAACCAAGCTGGAT GCAGTGGCACAATCTTGCTCACTGNCATCTCGNCTNCTGGGTTCAGCGATTTNCTGCCTC ACCTCCCGAGTGTTTGGATCCAGGCATGCATGACCAGCTCACTAATTTTGTTTTTGGTAG AGACGGGTTTACCATATTGNCAGC >OriGene 3' read for NM_031901 unedited
NGGNTACACTATGNNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTT GCAGACATGTGATTTGAGTTTATTGCAGAGAATGGTAATAGGAAATGGGATTGTACAAAG AAAAGAGATGGAGAGAAAACTGGATGAGGGTTTCGCACTGGGTGTCCCACCCACAGGCCT CAGCAGCCCTGCCACGGATCTGCCCGATTCTTTCGCATCAAGAAGTTGATCTTGCGAGCC ATTTCCATGTTGTAGATCCGCCGGCACCTTTCATAGCTTTCCCTCTGTCGCCGGCGGCAT GGCTTCTCATAATACCGCCGATGCTTAATGTCCTCAATGAGCCCATCCATAGTGAGGATT CTGTTTAGGGTCCTGTATGCGCTTTCCACGTTCCCTTCCTGTACCATCACAGTCCTGGCG ATGAACTTCAGATGTTTTGCCATGACCTTGGATTTAAACCTTCACTCTGTAGAGCCTCGC GCGCTCAGTACCTAGCCCACCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTAT TACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACG CCTACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACGACATTTTGGAAAGTCCCGTTG ATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGNTGGAGACTTGNAAATCCCC GTGAGTCAAACCGCTATCCACGCCCATTGGNTGTACTGNCCAAACCGCATCACCATGGTA ATAGCGATGACTAATACCTANATGTACTGCCCAGTANGAAAGTCCCGTAAAGTCATGTAC TGGGCATTATGCCAGGCGGGCCCATTACCGTCATTGACGTCAATAAGGGGCGGACTGGGC ATATGAACACTTGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_031901 |
| Insert Size | 600 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_031901.3, NP_114107.1 |
| RefSeq Size | 483 bp |
| RefSeq ORF | 264 bp |
| Locus ID | 54460 |
| UniProt ID | P82921 |
| Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice variants that differ in the 5' UTR; both transcripts encode the same protein. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) is the predominant transcript. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203193 | MRPS21 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein S21 (MRPS21), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 1200.00 |
|
| RC203193L3 | Lenti ORF clone of Human mitochondrial ribosomal protein S21 (MRPS21), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC203193L4 | Lenti ORF clone of Human mitochondrial ribosomal protein S21 (MRPS21), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG203193 | MRPS21 (tGFP-tagged) - Human mitochondrial ribosomal protein S21 (MRPS21), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 4370.00 |
