MT1X (BC032131) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	metallothionein 1X; MT-1l; MT1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC032131 edited CATCTGTCCCGCTGCGTGTTTTCCTCTTGATCGGGAACTCCTGCTTCTCCTTGCCTCGAA ATGGACCCCAACTGCTCCTGCTCGCCTGTTGGCTCCTGTGCCTGTGCCGGCTCCTGCAAA TGCAAAGAGTGCAAATGCACCTCCTGCAAGAAGAGTGAGTGCAGGGCCTTCCCTGCGAAT CTGGGGGATGGGCCAATTTAGAGCAGGGAACCCAGAGCTCTGCAGGCAGGGGCAGGCCAA TGACCAGCTTCCCCAAACCCCTCCTTCAACACCTGATTCAGAATCAGACCTCAAATTGCC TTAAAAATGGGCTGCTGCTCCTGCTGCCCTGTGGGCTGTGCCAAGTGTGCCCAGGGCTGC ATCTGCAAAGGGACGTCAGACAAGTGCAGCTGCTGTGCCTGATGCCAGGACAGCTGTGCT CTCAGATGTAAATAGAGCAACCTATATAAACCTGGATTTTTTTTTTTTTTGTACAACCCT GACCCGTTTGCTACATCTTTTTTTCTATGAAATATGTGAATGGCAATAAATTCATCTAGA CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC032131 unedited CCCCCGTCAGATTTTGTAAACGACTCACTATAGGCGGCCGCGCAATTCGCACGAGGCATC TGTCCCGCTGCGTGTTTTCCTCTTGGTGGGGAACTCCTGCTTCTCCTTGCCTCGAAATGG ACCCCAACTGCTCCTGCTCGCCTGTTGGCTCCTGTGCCTGTGCCGGCTCCTGCAAATGCA AAGAGTGCAAATGCACCTCCTGCAAGAAGAGTGAGTGCAGGGCCTTCCCTGCGAATCTGG GGGATGGGCCAATTTAGAGCAGGGAACCCAGAGCTCTGCAGGCAGGGGCAGGCCAATGAC CAGCTTCCCCAAACCCCTCCTTCAACACCTGATTCAGAATCAGACCTCAAATTGCCTTAA AAATGGGCTGCTGCTCCTGCTGCCCTGTGGGCTGTGCCAAGTGTGCCCAGGGCTGCATCT GCAAAGGGACGTCAGACAAGTGCAGCTGCTGTGCCTGATGCCAGGACAGCTGTGCTCTCA GATGTAAATAGAGCAACCTATATAAACCTGGATTTTTTTTTTTTTTGTACAACCCTGACC CGTTTGCTACATCTTTTTTTCTATGAAATATGTGAATGGCAATAAATTCATCTGGGCTAA NNNNAAANNNNNAAAAAAAAAAAAAGAAAAAACTCGACTCTAAATTGCGGCCGCGGTCAT AGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGGGACCCCTCCCAGTGCCTCTCCTG GCCCTGGAATTGCCACTCCAGTGCCCACCAGCCTTGTCCTAAAAAAATAAATTGCCTCAT TTTGGCTGACAAGGGGTGCTTCTATAAATTATGGGGGGAGGGGGTGGGGTATTTAACACA GGGGCCAATTTTAAAACAAACTTGTGGGGCCGGGGGGGTTTTTTGGAAACCAACTGAG
Restriction Sites	Please inquire
ACCN	BC032131
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC032131.2, AAH32131.1
RefSeq Size	574 bp
Locus ID	4501
Gene Summary	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. May be involved in FAM168A anti-apoptotic signaling (PubMed:23251525).[UniProtKB/Swiss-Prot Function]

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