MT1X (BC032338) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	metallothionein 1X; MT-1l; MT1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC032338 edited TCTGTCCCGCTGCGTGTTTTCCTCTTGATCGGGAACTCCTGCTTCTCCTTGCCTCGAAAT GGACCCCAACTGCTCCTGCTCGCCTGTTGGCTCCTGTGCCTGTGCCGGCTCCTGCAAATG CAAAGAGTGCAAATGCACCTCCTGCAAGAAGAGCTGCTGCTCCTGCTGCCCTGTGGGCTG TGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGACGTCAGACAAGTGCAGCTGCTGTGC CTGATGCCAGGACAGCTGTGCTCTCAGATGTAAATAGAGCAACCTATATAAACCTGGATT TTTTTTTTTTTTTTTTTGTACAACCCTGACCCGTTTGCTACATCTTTTTTTCTATGAAAT ATGTGAATGGCAATAAATTCATCTAGACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC032338 unedited GGGGGGTTCAGATTTGTAAACGACTCACTATAGGCGGCCGCGAATCAGATCTGGTACCGG TCCGGAATTCCCGGGATTCTGTCCCGCTGCGTGTTTTCCTCTTGATCGGGAACTCCTGCT TCTCCTTGCCTCGAAATGGACCCCAACTGCTCCTGCTCGCCTGTTGGCTCCTGTGCCTGT GCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCTCCTGCAAGAAGAGCTGCTGCTCC TGCTGCCCTGTGGGCTGTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGACGTCAGAC AAGTGCAGCTGCTGTGCCTGATGCCAGGACAGCTGTGCTCTCAGATGTAAATAGAGCAAC CTATATAAACCTGGATTTTTTTTTTTTTTTTTTTGTACAACCCTGACCCGTTTGCTACAT CTTTTTTTCTATGAAATATGTGAATGGCAATAAATTCATCTNGACTAANNAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGCGGCCG CGGTCATAGCTGTTTCCTGAACAAATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGC CTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAA GTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATATTATGGGGTGGAGGGGGTGGTT ATTGGAACAAAGGGCAAGTTTGGAAGACAACCTGTAGGGCCTGCGGGGTCTATTTGGAAC CAAGCTGNAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAG CGATTCTCCTGCCCCAGCCTCCCGAGTTGGTGGGGATCCAAGCCT
Restriction Sites	NotI-NotI
ACCN	BC032338
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC032338.1, AAH32338.1
RefSeq Size	454 bp
Locus ID	4501
Gene Summary	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. May be involved in FAM168A anti-apoptotic signaling (PubMed:23251525).[UniProtKB/Swiss-Prot Function]

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