WDR85 (DPH7) (BC003123) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C9orf112; FLJ90634; OTTHUMP00000022705; RP11-48C7.3; WD repeat domain 85
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC003123 edited GTCCGGCCGGCCCCGGCCTCGCCGCCCCGCGCAGTACCCAGCCCGGCCCCGCCGACCCGC CTCTACTGCCGGCTCCGCGCCCTTCCCCGAGGGCTGGATGATGGGCTGTTTCGCCCTGCA AACGGTGGACACCGAGCTGACCGCGGACTCGGTGGAGTGGTGCCCGCTGCAAGGCTGCAG GCACCTGCTGGCGTGCGGGACCTACCAGCTGCGGCGGCCGGAGGACCGGCCTGCCGGCCC CCAGAACAAGGGTGGAATGGAAGTTAAGGAGCCTCAGGTCCGTTTAGGCCGTCTCTTCCT GTACAGTTTCAATGACAACAACTCTATTCACCCTCTGGTCGAGGTCCAAAGAAAAGATAC TTCTGCAATCCTGGACATGAAATGGTGTCACATCCCGGTGGCTGGACATGCCCTCTTGGG CTTGGCAGATGCCAGTGGATCCATACAACTGCTCCGCCTGGTGGAATCTGAGAAGAGCCA CGTGCTGGAGCCATTGTCCAGCCTTGCCCTGGAGGAGCAGTGTCTGGCTTTGTCCCTAGA TTGGTCCACTGGGAAAACTGGAAGGGCCGGGGACCAGCCCTTGAAGATCATCAGCAGTGA CTCCACAGGGCAGCTCCACCTCCTGATGGTGAATGAGACGAGGCCCAGGCTGCAGAAAGT GGCCTCATGGCAGGCACATCAATTCGAGGCCTGGATTGCTGCTTTCAATTACTGGCATCC AGAAATTGTGTATTCAGGGGGCGACGATGGCCTTCTGAGGGGCTGGGACACCAGGGTACC CGGCAAATTTCTCTTCACCAGCAAAAGACACACCATGGGTGTGTGCAGCATCCAGAGCAG CCCTCATCGGGAGCACATCCTGGCCACGGGAAGCTATGATGAACACATCCTACTGTGGGA CACACGAAACATGAAGCAGCCGTTGGCAGATACGCCTGTGCAGGGTGGGGTATGGAGAAT CAAGTGGCACCCTTTCCACCACCACCTGCTCCTGGCCGCCTGCATGCACAGTGGCTTTAA GATCCTCAACTGCCAAAAGGCAATGGAGGAGAGGCAGGAGGCGACGGTCCTGACATCTCA CACATTGCCCGACTCGCTGGTGTATGGAGCCGACTGGTCCTGGCTGCTCTTCCGTTCTCT GCAGCGGGCCCCCTCGTGGTCCTTTCCTAGCAACCTAGGAACCAAGACGGCAGACCTGAA GGGTGCAAGCGAGTTGCCAACACCCTGTCATGAATGCAGAGAGGATAACGATGGGGAGGG CCATGCCAGACCCCAGAGTGGAATGAAGCCACTCACAGAGGGCATGAGGAAGAATGGCAC CTGGCTGCAGGCTACAGCAGCCACCACACGTGACTGTGGCGTGAACCCAGAAGAAGCAGA CTCAGCCTTCAGCCTCCTGGCCACCTGCTCCTTCTATGACCATGCGCTCCACCTCTGGGA GTGGGAGGGGAACTGAGCTTGAAATCATGAAGCCCCTTCCCACAAGGAAACCAGGAGGGA GACTGCGAGTGAGTGCCCGGGACCACCTCATCAGAGATGCTTACTGCAGCCCTGCAGGTG CCTGGGCACTGATGGAATCCACAGTGTAGTCAGAAAAGCTGTTGACTTCTCTTAAATCAG CTTCCCTGCTGGGCCCCTGAAAGTGGACTGGGTGATTCTGTCTGGCAGAGAGTGGGGAAA AGACGCGGTTTCCAGCTTGCAGATTTGTTAAGTTTCTCAGGCAGATTTTGACTTTCAGCC TTTCATACTTGTTTAAGCAACTATTTGTATTAAATGAAGTTTTTTGAAAAAAAAAAAAAA AAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC003123 unedited TCGTATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGGTCCGGCC GGCCCCGGCCTCGCCGCCCCGCGCAGTACCCAGCCCGGCCCCGCCGACCCGCCTCTACTG CCGGCTCCGCGCCCTTCCCCGAGGGCTGGATGATGGGCTGTTTCGCCCTGCAAACGGTGG ACACCGAGCTGACCGCGGACTCGGTGGAGTGGTGCCCGCTGCAAGGCTGCAGGCACCTGC TGGCGTGCGGGACCTACCAGCTGCGGCGGCCGGAGGACCGGCCTGCCGGCCCCCAGAACA AGGGTGGAATGGAAGTTAAGGAGCCTCAGGTCCGTTTAGGCCGTCTCTTCCTGTACAGTT TCAATGACAACAACTCTATTCACCCTCTGGTCGAGGTCCAAAGAAAAGATACTTCTGCAA TCCTGGACATGAAATGGTGTCACATCCCGGTGGCTGGACATGCCCTCTTGGGCTTGGCAG ATGCCAGTGGATCCATACAACTGCTCCGCCTGGTGGAATCTGAGAAGAGCCACGTGCTGG AGCCATTGTCCAGCCTTGCCCTGGAGGAGCAGTGTCTGGCTTTGTCCCTAGATTGGTCCA CTGGGAAAACTGGAAGGGCCGGGGACCAGCCCTTTGAGATCATCAGCAGTGACTCCACAG GGCAGCTCCACCTCCTGATGGTGAATGAGACGAAGCCCANGCTGCAGAAAGTGGCCTCAT GGCAGGCACATCANTTCGAGGCCTGGATTGCTGCTTTCATTACTGGCATCCAGAAATGTG TATTCAGGNNGCGACNATAGCCCTTCTGTAGGGCTGGGACACCAGGGNTACCCCGGCAAA TTNTTCTTCACAAGCAAAGAACACCCATGGGTGTGTGCAGCATCCAAAGCAGCCCCTCAT GNGGAGCCCTATCCTGTCCACCGGAAAGCTATGGATGAACACCTCCTACTGTGTGGAACA ACGAAACTGAACCAGCCCTT
Restriction Sites	Please inquire
ACCN	BC003123
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003123.1, AAH03123.2
RefSeq Size	1808 bp
Locus ID	92715
Gene Summary	Diphthamide is a post-translationally modified histidine residue present in elongation factor 2, and is the target of diphtheria toxin. This gene encodes a protein that contains a WD-40 domain, and is thought to be involved in diphthamide biosynthesis. A similar protein in yeast functions as a methylesterase, converting methylated diphthine to diphthine, which can then undergo amidation to produce diphthamide. [provided by RefSeq, Oct 2016]

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