BRUNOL4 (CELF4) (BC045711) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bruno-like 4, RNA binding protein; Bruno -like 4, RNA binding protein; bruno-like 4, RNA binding protein (Drosophila); BRUNOL-4; CELF4; CUG-BP and ETR-3 like factor 4; LYST-interacting protein LIP9; RNA-binding protein BRUNOL4
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC126602 sequence for BC045711 edited (data generated by NextGen Sequencing) ATGTATATAAAGATGGCCACGTTAGCAAACGGACAGGCTGACAACGCAAGCCTCAGTACC AACGGGCTCGGCAGCAGCCCGGGCAGTGCCGGGCACATGAACGGATTAAGCCACAGCCCG GGGAACCCGTCGACCATTCCCATGAAGGACCACGATGCCATCAAGCTGTTCATTGGGCAG ATCCCCCGCAACCTGGATGAGAAGGACCTCAAGCCCCTCTTCGAGGAGTTTGGCAAAATC TACGAGCTTACGGTTCTGAAGGACAGGTTCACAGGCATGCACAAAGGCTGCGCCTTCCTC ACCTACTGCGAGCGTGAGTCAGCGCTGAAGGCCCAGAGCGCGCTGCACGAGCAGAAGACT CTGCCCGGGATGAACCGGCCGATCCAGGTGAAGCCTGCGGACAGCGAGAGCCGAGGAGAN GATAGAAAACTCTTCGTGGGCATGCTCAACAAGCAACAGTCCGAGGACGACGTGCGCCGC CTTTTCGAGGCCTTTGGGAACATCGAGGAGTGCACCATCCTGCGCGGGCCCGACGGCAAC AGCAAGGGGTGCGCCTTTGTGAAGTACTCCTCCCACGCCGAGGCGCAGGCCGCCATCAAC GCGCTACACGGCAGCCAGACCATGCCGGGAGCCTCGTCCAGTCTGGTGGTCAAGTTCGCC GACACCGACAAGGAGCGCACGATGCGGCGAATGCAGCAGATGGCTGGCCAGATGGGCATG TTCAACCCCATGGCCATCCCTTTCGGGGCCTACGGCGCCTACGCTCAGGCACTGATGCAG CAGCAAGCGGCCCTGATGGCATCAGTCGCGCAGGGCGGCTACCTGAACCCCATGGCTGCC TTCGCTGCCGCCCAGATGCAGCAGATGGCGGCCCTCAACATGAATGGCCTGGCGGCCGCA CCTATGACCCCAACCTCAGGTGGCAGCACCCCTCCGGGCATCACTGCACCAGCCGTGCCT AGCATCCCATCCCCCATTGGGGTGAATGGCTTCACCGGCCTCCCCCCACAGGCCAATGGG CAACCTGCTGCGGAAGCTGTGTTCGCCAATGGCATCCACCCCTACCCAGCACAGAGCCCC ACCGCCGCGGACCCCCTGCAGCAGGCCTACGCCGGAGTGCAGCAGTATGCAGGTCCAGCT GCCTACCCTGCTGCCTATGGTCAGATAAGCCAGGCCTTTCCTCAGCCGCCTCCAATGATC CCCCAGCAGCAGAGAGAAGGCTTCGTGAGCTTCGACAACCCGGCCAGCGCGCAGACCGCC ATCCAGGCCATGAACGGCTTCCAGATCGGCATGAAGAGGCTCAAGGTGCAGCTGAAGCGG CCCAAAGACGCCAATCGCCCGTACTGA Clone variation with respect to BC045711.1 420 a=>n 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC045711 unedited TGTAATCACGACTCTCTATAGNNGGCGGCCGCGCAATTCGGCACGAGGGAGAGCAGAGAG CGAGCGGAGAGCGAGGTGTAGAGAAACCGAGGGGGAGAGAACCCGAGTGTGTGTATGCGT GTGCGTGTGTGAGCGCGAGCGAGCGAGCGAGAGAGAGGAGCGAGAGAGTGTGAGCGAGAA AGAATAAAAGGAAAGAAGATTTTCTCTATGTATATAAAGATGGCCACGTTAGCAAACGGA CAGGCTGACAACGCAAGCCTCAGTACCAACGGGCTCGGCAGCAGCCCGGGCAGTGCCGGG CACATGAACGGATTAAGCCACAGCCCGGGGAACCCGTCGACCATTCCCATGAAGGACCAC GATGCCATCAAGCTGTTCATTGGGCAGATCCCCCGCAACCTGGATGAGAAGGACCTCAAG CCCCTCTTCGAGGAGTTTGGCAAAATCTACGAGCTTACGGTTCTGAAGGACAGGTTCACA GGCATGCACAAAGGCTGCGCCTTCCTCACCTACTGCGAGCGTGAGTCAGCGCTGAAGGCC CAGAGCGCGCTGCACGAGCAGAAGACTCTGCCCGGGATGAACCGGCCGATCCAGGTGAAG CCTGCGGACAGCGAGAGCCGAGGAGATAGAANACTCTTCGTGGGCATGCTCAACAAGCCA CAGTCCGAGGACGACGTGCGCCGCCTTTTCGAGGCCTTTGGGAACATCGAGGAGTGCACC ATCCTGCGCGGGCCCGACGGCACAGCAAGGGTGCGCCTTTGTGAAGTACTCCTCCCACGC CGAGCGCAGGCCGNNCATCACGCGCTACACGGGAGCCAGACCATGCCCGGNAGCCTCGTT CAGTCTGTGGGGCAAGTCCGCCGCAACGACAAGGAGCGCACGATGCGCGAATGCANANAT GGCTTGCCAAATGGCC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC045711 unedited NTAGCTATGNACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCATCTT CTTCTTCATGTCATATATATTTTCCCCCAAACACGTGCCCTCTGAACTCCATAGACGCTA TACTTTCCTTGAAGAAATGTTACAGTCACACAGACAGTGTCTGGAGTCTTCAGCTTGATT GATATTGGCTGATATGTCAAAGGTGTCATCCAACAGTTCTCATTTATAAATATATATAGA GAGAGGTTTGTTTTTTAATGTAGCCCGTTCAGCATCCTGCCCTGTGCGAGTCCTGGTCTC CCCCGGGGGACGCTCCCGCCGGCGCTCAGTACGGGCGATTGGCGTCTTTGGGCCGCTTCA GCTGCACCTTGAGCCTCTTCATGCCGATCTGGAAGCCGTTCATGGCCTGGATGGCGGTCT GCGCGCTGGCCGGGTTGTCGAAGCTCACGAAGCCTTCTCTCTGCTGCTGGGGGATCATTG GAGGCGGCTGAGGAAAGGCCTGGCTTATCTGACCATAGGCAGCAGGGTAGGCAGCTGGAC CTGCATACTGCTGCACTCCGGCGTAGGCCTGCTGCAGGGGGTCCGCGGCGGTGGGGCTCT GTGCTGGGTAGGGGTGGATGCCATTGGCGAACACAGCTTCCGCAGCAGGTTGCCCATTGG CCTGTGGGGGGAGGCCGGTGAAGCCATTCCCCCCATGGGGGATGGGATGCTAGGCACAGC TGGTGCAGTGATGCCCGGAGGGGTGCTGCCACCTGAGTTNGGGGTCATTAGTGCCGGCCG CCAGCCATTATGTTTGAGGGCCGCCTTTGCTGCATTGGGCCGCAACGAAGCACCATGGGG TTAAGGTACCCGCCCTGCCCATGAGCCACTAGGGCCGGTTGCTGCTGATTATGCCTGAAC GTAAGCCCCGAGCCCCAAAAGAATGCCCCTGGGGTGAAAATCCCCTTGGCCCACCTTGCT
Restriction Sites	NotI-NotI
ACCN	BC045711
Insert Size	1850 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC045711.1, AAH45711.1
RefSeq Size	2104 bp
RefSeq ORF	1347 bp
Locus ID	56853
Gene Summary	Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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