MT ATP6 (ATP6) (NM_173702) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ATP6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC126708 representing NM_173702. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAACGAAAACCTGTTCGCGTCTTTTATCGCGCCGACCATTCTGGGTCTGCCGGCTGCGGTACTGATC ATTCTGTTTCCGCCGCTGCTGATCCCGACCTCTAAATATCTGATTAACAACCGTCTGATCACCACTCAG CAGTGGCTGATCAAACTGACTTCTAAACAGATGATGACCATGCATAACACCAAAGGTCGTACCTGGTCT CTGATGCTGGTTTCCCTGATCATCTTCATCGCGACTACTAACCTGCTGGGCCTGCTGCCGCACTCTTTC ACCCCGACTACCCAGCTGTCCATGAACCTGGCGATGGCGATTCCGCTGTGGGCGGGTACCGTTATCATG GGTTTCCGTTCTAAAATCAAAAACGCGCTGGCGCACTTCCTGCCGCAGGGCACTCCGACCCCGCTGATT CCGATGCTGGTAATTATCGAAACCATCTCCCTGCTGATCCAGCCGATGGCACTGGCGGTTCGTCTGACT GCGAACATTACTGCGGGTCATCTGCTGATGCACCTGATTGGTTCTGCTACTCTGGCTATGAGCACCATC AACCTGCCGTCTACCCTGATTATCTTTACCATCCTGATTCTGCTGACTATCCTGGAAATCGCGGTTGCA CTGATTCAGGCGTACGTGTTTACTCTGCTGGTGTCCCTGTACCTGCACGACAACACTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_173702
Insert Size	681 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_173702.1
RefSeq Size	843 bp
RefSeq ORF	681 bp
Locus ID	4508
Protein Families	Transmembrane
Protein Pathways	Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
MW	24.8 kDa
Gene Summary	Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Key component of the proton channel; it may play a direct role in the translocation of protons across the membrane. [UniProtKB/Swiss-Prot Function]

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