eIF2B epsilon (EIF2B5) (AK056400) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CACH; CLE; EIF-2B; EIF2Bepsilon; LVWM
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK056400, the custom clone sequence may differ by one or more nucleotides AAAAAGAGAAAAAGGTGCTGCTGCGCCTTTTCAAGCCTTGGACCTTTACGCCCGCACTTTCCTCTGCCCA GAAAGCTTACTGCTTAACTCCAGTACAACTTACTGGTGCGCCTGGATCCACCCCCGTCTCCACTCTGCTT AAGACACAGGAGGCTGATAGGCTGGAAGAATGAATAGATGACATAAAATAGTCATTGTTATCCCCACATA ACTTGGCAGCAAGATTGTGGAACTCTGCAGTCAGACAGATCGGGTTCCAATACCAGTTCTTCCACTTACT AGCTGGGTAAACTTCCTTCATCCTTCTGAGAGTTCAAGAAACGTTTGCTGAACGAATGAATAAGATGTTT TGCAGAGATAATGCTTGTATCGTGCCTGGCCCAAAATCAGCGCTCAATGGAATTACTGGTCTCCCTACTA TATGCCTGCCACCTTCTCTATTTATGCAGGTTCTGAGTTCCTCGCAGCGGGGGAACATCTGGCTCGGTTC TATCTCCCCAAGGCGCTGGGTTCCCAGAAGTTTCTAGAATGAATACGGAGGGCTACATATTTCACATGCG CGGGGCGGGGAAACTGCTGGGCGATTACAGATTCCCGATGGAGCTGGCCCCTTCGTGGCGACCACAAGTC CTATCACGCCACAAGAATCTCGGCTTGTTCACCTACAAATGAAGGCCGACGATACTTGCAGACCGAGTCT GACATCTATGGGACCCCACTCCTACCCTGCGCCTCAGGGGCCGGGACAACCGTGACCCGTACCGACTCCT CGCGCCTCCGGGATCAACCTCAGACCCCTGTGGGCTCCCGGGGAAGACGCATGCGCACTGCTCCCTCCTC GTCTCCTTCCCTTCGTTAATTAGTCCATCGCATTTCAAGTGCTGGATCCTTTTTGTCCCCTACTGCGTGC GGTGGCAGCTTCCTTGCGGAAGTGGTGACCGTGAGAGAAGAAGATGGCGGCCCCTGTAGTGGCGCCGCCT GGTGTGGTGGTTAGTCGGGCTAACAAGCGCAGCGGCGCGGGGCCGGGAGGCAGCGGTGGCGGGGGAGCCA GAGGGGCGGAGGAGGAACCGCCGCCGCCCCTACAAGCAGTTCTGGTGGCCGATAGCTTCGATCGCCGCTT CTTCCCCATCTCCAAGGACCAGCCTCGGGTGAGCGCCGCGCACGCGAGCAGCCAGAGGGCAGGAAGGGTA GCAGGGCTGGAGCAAGTTCTCATTTCGGCTAACTACAACAGCATGCCCTTGAAGGACCTGCGTCTATGCT GTTATCCTAAAACCGGATGCAGAGGGACTGTCTAAACCCTGATGACTGCTGACCAAGTTAGTGGCCGGCA GATTTGGGCATTCGTAATGCTGTCCACCTCGGGTTTGAAAGGAATGAAGAGAGCTAAATGTAGAAACAGC GCTTGAGAGAAGCCTTCACGCGGGGATTCAGTGCCCCTAGAAGAGGCTGCGCCGCTTTTAACCTTGCCGA TGACGGTTGCTTAGGTGAAAAGAAACGACCTTTTGCCTCTGAAAGGGCGGTAATATGCCTTAAAAAGAGC GGGCTAGATCTCAGGCAGTGTATTTTTTTTTCCTTCCAGGTTCTAATGATAGCCGGTGCTATCTGTCGAA AGGACAAAATGGGTTTAGTGGTTGATTGCCATCAGCCCCCGAGTTGTCTAGACAATGGGGTATTGGACTC AGCTGGGTCGTTCAGCAGTCAGTTCAGAAAGAGGGAACTGCATTGGATTTAAGGAAAATCCTCTCTAATT CCCTCGAAAAAGCCTAATGGCTGTTCATTTTTGACAAGTGACGGGAGATGGCTAACCTCTGACGCTTAGA AAGGGATCAAGGAAGGAAATCTTATGGGGAAGAAGGCCTTAGCAATGCTTAAATTTATGGAAATATGATT GATTTCCAGTATTATCCTTTGCCAGCAGTGAACTGCCATTCTGTCACAGCTCTGTGTCTGATACAAGGCA CATAGATTCTGTACTTACCATCCCCAAATTGCAATGTCTCAGACTCAGGCTTAGAGCATGGCATGAACAT CAAAGGCAGGAACCTGTTTATCTTTGAATTGGAAAGATACAGCAAAATTACACTGTTTGGAAATACGAAT AGAGGAGTGAAAATTGTTGCAGTGGGGTACCCAAAGGGATTCGAGACCTCAAGTTTTTTTTCATCTTGTA TCCTTCAGGTCCTCTTGCCCCTGGCCAATGTGGCATTAATTGACTACACTCTGGAATTCCTGACTGCCAC AGGTGTACAGGAAACATTTGTCTTTTGTTGCTGGAAAGCTGCTCAAATCAAAGAACATTTACTGTAAGGC CCTGCAACTTTTCTTTCCATGTTTCGCCATCTTTTTCCAGTTTTTTCAGGATGAATGTAACTAAGGGGAA ATGTGGGAGGGGAAAAATGTGTCTACTTAGATGTCATTGTAAGTTCTAAGGGTATTTTCTACACTTATCA GGAGGTGAGCAGCTGAAACTTTGTATCTGTCTGTCTTGGGTTTTCTGTGACAAAGATTAAAGAATAAACT TGGGTTTTTTCTGAATCTCATT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK056400 unedited NGGGGGACCCCCCCTNTCTCCCCCNNNNGGGTTCGCATTTTGTAATACGACTTTTATAGG GCGGCCGCGAATTCGCACGAGGATGGCGGCCCCTTTTTTGGCGCCGCCTGGTGTGGTGGT TAGTCGGGCTAACAAGCGCAGCGGCGCGGGGCCGGGAGGCAGCGGTGGCGGGGGAGCCAG AGGGGCGGAGGAGGAACCGCCGCCGCCCCTACAAGCAGTTCTGGTGGCCGATAGCTTCGA TCGCCGCTTCTTCCCCATCTCCAAGGACCAGCCTCGGGTGAGCGCCGCGCACGCGAGCAG CCAGAGGGCAGGAAGGGTGGCAGGGCTGGAGCAAGTTCTCATTTCGGCTAACTACAACAG CATGCCCTTGAAGGACCTGCGTCTATGCTGTTATCCTAAAACCGGATGCAGAGGGACTGT CTAAACCCTGATGACTGCTGACCAAGTTAGTGGCCGGCAGATTTGGGCATTCGTAATGCT GTCCACCTCGGGTTTGAAAGGAATGAAGAGAGCTAAATGTAGAAACAGCGCTTGAGAGAA GCCTTCACGCGGGGATTCAGTGCCCCTAGAAGAGGCTGCGCCGCTTTTAACCTTGCCGAT GACGGTTGCTTANGTGAAAAGAAACGACCTTTTGCCTCTNGAAGGGCCGTAATATGCCTT TAAAAGAGCGGGCTAGATCTCANGCAGTGTATTTTTTTTTTTCCTTCCAGTTCTAATGAT AGCCCGGGCTATCTGTCGAAAGGACAAATGGGTTTAGTGGTTGATTGCATCAGCCCCCGA GTGTCTAACATGGGGTATTGGACTCANCTGGTCGTTCACAGTCATTTAGAAAGAGGGACT GCATTGATTTAGGGAAATCCTTTTTATTCCTTCAAAAGCTATAGCTGTTATTTTTGCCAT GACCGGAATGGTTCCTTTGCCCTTAAAGGGTTCAGGAANAATCTTTGGGGAAAAGCCTTA CCAGCTTT
Restriction Sites	NotI-NotI
ACCN	AK056400
Insert Size	1700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	A TrueClone.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK056400.1
RefSeq Size	2542 bp
RefSeq ORF	2542 bp
Locus ID	8893
Gene Summary	This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq, Nov 2009]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.