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PICALM (NM_007166) Human Untagged Clone
CAT＃: SC127391
PICALM (untagged)-Human phosphatidylinositol binding clathrin assembly protein (PICALM), transcript variant 1
CNY 7,288.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CALM; CLTH; LAP |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_007166, the custom clone sequence may differ by one or more nucleotides
ATGTCCGGCCAGAGCCTGACGGACCGAATCACTGCCGCCCAGCACAGTGTCACCGGCTCTGCCGTATCCA AGACAGTATGCAAGGCCACGACCCACGAGATCATGGGGCCCAAGAAAAAGCACCTGGACTACTTAATTCA GTGCACAAATGAGATGAATGTGAACATCCCACAGTTGGCAGACAGTTTATTTGAAAGAACTACTAATAGT AGTTGGGTGGTGGTCTTCAAATCTCTCATTACAACTCATCATTTGATGGTGTATGGAAATGAGCGTTTTA TTCAGTATTTGGCTTCAAGAAACACGTTGTTTAACTTAAGCAATTTTTTGGATAAAAGTGGATTGCAAGG ATATGACATGTCTACATTTATTAGGCGGTATAGTAGATATTTAAATGAGAAAGCAGTTTCATACAGACAA GTTGCATTTGATTTCACAAAAGTGAAGAGAGGGGCTGATGGAGTTATGAGAACAATGAACACAGAAAAAC TCCTAAAAACTGTACCAATTATTCAGAATCAGATGGATGCACTTCTTGATTTTAATGTTAATAGCAATGA ACTTACAAATGGGGTAATAAATGCTGCCTTCATGCTCCTGTTCAAAGATGCCATTAGACTGTTTGCAGCA TACAATGAAGGAATTATTAATTTGTTGGAAAAATATTTTGATATGAAAAAGAACCAATGCAAAGAAGGTC TTGACATCTATAAGAAGTTCCTAACTAGGATGACAAGAATCTCAGAGTTCCTCAAAGTTGCAGAGCAAGT TGGAATTGACAGAGGTGATATACCAGACCTTTCACAGGCCCCTAGCAGTCTTCTTGATGCTTTGGAACAA CATTTAGCTTCCTTGGAAGGAAAGAAAATCAAAGATTCTACAGCTGCAAGCAGGGCAACTACACTTTCCA ATGCAGTGTCTTCCCTGGCAAGCACTGGTCTATCTCTGACCAAAGTGGATGAAAGGGAAAAGCAGGCAGC ATTAGAGGAAGAACAGGCACGTTTGAAAGCTTTAAAGGAACAGCGCCTAAAAGAACTTGCAAAGAAACCT CATACCTCTTTAACAACTGCAGCCTCTCCTGTATCCACCTCAGCAGGAGGGATAATGACTGCACCAGCCA TTGACATATTTTCTACCCCTAGTTCTTCTAACAGCACATCAAAGCTGCCCAATGATCTGCTTGATTTGCA GCAGCCAACTTTTCACCCATCTGTACATCCTATGTCAACTGCTTCTCAGGTAGCAAGTACATGGGGAGAT CCTTTCTCTGCTACTGTAGATGCTGTTGATGATGCCATTCCAAGCTTAAATCCTTTCCTCACAAAAAGTA GTGGTGATGTTCACCTTTCCATTTCTTCAGATGTATCTACTTTTACTACTAGGACACCTACTCATGAAAT GTTTGTTGGATTCACTCCTTCTCCAGTTGCACAGCCACACCCTTCAGCTGGCCTTAATGTTGACTTTGAA TCTGTGTTTGGAAATAAATCTACAAATGTTATTGTAGATTCTGGGGGCTTTGATGAACTAGGTGGACTTC TCAAACCAACAGTGGCCTCTCAGAACCAGAACCTTCCTGTTGCCAAACTCCCACCTAGCAAGTTAGTATC TGATGACTTGGATTCATCTTTAGCCAACCTTGTGGGCAATCTTGGCATCGGAAATGGAACCACTAAGAAT GATGTAAATTGGAGTCAACCAGGTGAAAAGAAGTTAACTGGGGGATCTAACTGGCAACCAAAGGTTGCAC CAACAACCGCTTGGAATGCTGCAACAATGGCACCCCCTGTAATGGCCTATCCTGCTACTACACCAACAGG CATGATAGGATATGGAATTCCTCCACAAATGGGAAGTGTTCCTGTAATGACGCAACCAACCTTAATATAC AGCCAGCCTGTCATGAGACCTCCAAACCCCTTTGGCCCTGTATCAGGAGCACAGATACAGTTTATGTAA >OriGene 5' read for NM_007166 unedited
GCGGCCCGCGAATTCGCACGAGGGTGCGGTGGGGGGTGGGGACCCTCCGGCTCTTGGGGG TCCCAGTCCCCGCCGGCTGCTGAGCGGGTGGGGTGGTGGAGGAGCTGCAGAGATGTCCGG CCAGAGCCTGACGGACCGAATCACTGCCGCCCAGCACAGTGTCACCGGCTCTGCCGTATC CAAGACAGTATGCAAGGCCACGACCCACGAGATCATGGGGCCCAAGAAAAAGCACCTGGA CTACTTAATTCAGTGCACAAATGAGATGAATGTGAACATCCCACAGTTGGCAGACAGTTT ATTTGAAAGAACTACTAATAGTAGTTGGGTGGTGGTCTTCAAATCTCTCATTACAACTCA TCATTTGATGGTGTATGGAAATGAGCGTTTTATTCAGTATTTGGCTTCAAGAAACACGTT GTTTAACTTAAGCAATTTTTTGGATAAAAGTGGATTGCAAGGATATGACATGTCTACATT TATTAGGCGGTATAGTAGATATTTAAATGAGAAAGCAGTTTCATACAGACAAGTTGCATT TGATTTCACAAAAGTGAAAAGAGGGGCTGATGGAGTTATGAGAACAATGAACACCAGAAA ACTCCTAAAAACTGTCCCAATTATTCAGATCAAATGGATGCCCCTCCTTGGATTTTAAGT TAAATACCAATGGAACCTACCAAAGGGGGAAAAAAAGGCTGCCCTTAAGGGCCCCTGGTA AAAAAGGCCCTTAAAACTGGTGGGCCCTTACCAGGGAGGGGATTTTTAATTTTGTGGGAA AAAAATTTTTGTGTTTAAAAAAAAACCCTGCCCAAAAGGGTCTCTCCCTCTTTTAAAAAT TCCCCCCTTAGGGAAACAAA >OriGene 3' read for NM_007166 unedited
NNNNNNNNCCCCCCCCCCCCCNNCCCCCNCCCCCCCCNNCCTTTGACTTTGGAACGCGGN CCGCNTACTAAGATCGAGTTTTTTTTTTTTTTTTTTGGGGGAACTGCATTTTATTCTGAA AATCCACCCATTTTGCTAACATACATTTTAATATTGTAAACAAAAATAGAATCTGCAGAG ACAATGCAACAAGAATGCTTAAACTCATGTACAGAATTGCTTTCCTACAATGAACTGTCC TTCTTAAGGCCCCTCTCCACCCAAATGTTAAAGATGTATTTACACAAAGCACCGATCAAC AGTGCAGTTTAATTCTTCGTTTACTAAACTCTTGGCTAGACATTAACTTTAAAGATACTA TTTCCCCTTATTTAAAAGGTATATGATCATAACATGGCACGAGCCAAAGAAAATGTTCAA GGACTTTGCCCCCCTTCCCTCCCCCTTGTAACACCTTCTAGAGTTTTACTGAAAAGAAGA AAGTCTTGAAGTGAAAGCAATTTCTCACATTCATTTGGAAAGGCCCTATGTCAAATGGAA AAAAATGACCTGCCCAGCACAAAGCAGTAAAGACCCTTCCCAAGCCCTAATGCTGAAATT AAAAGGAAGGGCTTTTCCTAATCAAGGAACGTTCCCTGGCAAAACCTCTAGCCAAAAATT GCCTTATAGGTTAAAGGCCTTAAAAGGGCTCCCCCAAAACCCACAAAGGGCCGGAAATTT TTAAAAAATTCCAACCCCTGGCCCTGGCTTTAAAAAATTAAAGGGCTCTTTTTGGAAACT CAAAAAAAACTCTCCAAAAAAAACCCTTTTTTGGGGGAAAAAAAAAAAAAAAACGGGGGC CATCCCCATCTCCTTTTTTTTAAAGGGGGGCCGGGAACAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_007166 |
| Insert Size | 3630 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007166.2, NP_009097.2 |
| RefSeq Size | 3860 bp |
| RefSeq ORF | 1959 bp |
| Locus ID | 8301 |
| UniProt ID | Q13492 |
| Domains | ENTH |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(10;11)(p13;q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The role of PTB domain containing adaptor proteins on PICALM-mediated APP endocytosis and localization
,Merthan, L;Haller, A;Thal, DR;von Einem, B;von Arnim, CAF;,
Biochem. J.
,PubMed ID 31300465
[PICALM]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213791 | PICALM (Myc-DDK-tagged)-Human phosphatidylinositol binding clathrin assembly protein (PICALM), transcript variant 1 |
CNY 7,280.00 |
|
| RC213791L1 | Lenti ORF clone of Human phosphatidylinositol binding clathrin assembly protein (PICALM), transcript variant 1, Myc-DDK-tagged |
CNY 9,680.00 |
|
| RC213791L2 | Lenti ORF clone of Human phosphatidylinositol binding clathrin assembly protein (PICALM), transcript variant 1, mGFP tagged |
CNY 9,680.00 |
|
| RC213791L3 | Lenti ORF clone of Human phosphatidylinositol binding clathrin assembly protein (PICALM), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213791L4 | Lenti ORF clone of Human phosphatidylinositol binding clathrin assembly protein (PICALM), transcript variant 1, mGFP tagged |
CNY 9,680.00 |
|
| RG213791 | PICALM (tGFP-tagged) - Human phosphatidylinositol binding clathrin assembly protein (PICALM), transcript variant 1 |
CNY 8,880.00 |
