LOC390714 (BC024289) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC024289, the custom clone sequence may differ by one or more nucleotides CCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACGGAGCACCGAGGATTCACCATGGAAC TGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTC TGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGT AGCTATAGCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTCATCGATGAGTAGTA GTAGTAGTTACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAA CTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATTACTGTGCGAGAGATCTC CGCCAGCTAACTTCCTACTGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGCCT CCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCT GGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGC GGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGC CCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGA CAAGAAAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAACTCCTG GGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGG TCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGT GGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC CTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCC CAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCC CCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGC GACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGG ACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGT CTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCG GGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCCCAGGCTCTCGGGGTCGCGCGAGGATGCTTGGCA CGTACCCCGTGTACATACTTCCCGGGCGCCCAGCATGGAAATAAAGCACCCAGCGCTGCCCTGGGCCCCT GCAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC024289 unedited GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTAGCCCTGGATTCCA AGGCCTATCCACTTGGTGATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTC CGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAG TCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGA TTCACCTTCAGTAGCTATAGCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAG TGGGTCTCATCCATTAGTAGTAGTAATAGTTACATATACTACGCAGACTCAGTGAAGGGC CGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTG AGAGCCGAGGACACGGCTGTATATTACTGTGCGAGAGATAGTCACCGGGACTTTGACTAC TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTC CCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGNGCTGCCTGGTC AAGGACTACTTCCCCGAACCGGTNGACGGTGTCGTGGAACTCANGCGCCCTGACCAGCGG CGTGCACACCTTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCACAACGTGGT GACCGTGCCCTTCAGCAGCTTGGGCACCCAGACCTANCATCTGCACGTGAATCACAAGCC CAGCACACCCNAGGTGACCAAGAAGAGTTGAGCCCANATCTTGTGACAAACTTACACATG CCACCGTGCCCAGACTGACTTCCTGTGGGGGGAACCCGTAAGTNTTTCCTTTTTNCCCCA NACCCANGACACCCNATGATCTCCGCACCCCTGAGACAGGGG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC024289 unedited NNTTTAGCTCTGNACCGCGGCCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTCCCAGGCAGGGGGGGGGGCTTTATTTCCATGCTGGGTGCCTGGGAAGT ATGTAAACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCAAAAGCCCGGGGAGCGGGGG CTTGCCGGCCGTCGCACTCATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGGGG TTGGGCAAAGCCTCATGCATCACGGAGCATGAAAAGACGTTCCCCTGCTGCCACCTGCTC TTGTCCACGGGGAGCTTGCTATAAAGGAAAAAGGAGCCGTCGGAGTCCAGCACGGGAGGC GTGGCCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTG GGATAAAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCATCTCCTCCCGG GATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT TTCTCGATGGGGGCTGGGAGGGCTTTGTTGGACACCTTGCACTTGTACTCCTTGCCATTC AGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGNACGGGCCTGTGAACTGC TCCTCCCGCGGCTTTTGTCTGGGATTATGCACCTCCACGCCGCCCACGTACCAGTTGAAC TTGGCCTCAAGGTCTTCGTGCCTCACGTCCACCACCACGCTGGGACCTCAGGGGTCCGGG AGACATGAGGGCGGCCCTGGCTTTGGGGGGAAAAAGAAAAATGACGGTCCCCCCAGGAGT CAAGGGGCTGGCCCCGGGGGCTCGTGGAAATTTTGCCCCAATATTGGCCCTCAACCCTTT GTCACCTTGGG
Restriction Sites	NotI-NotI
ACCN	BC024289
Insert Size	1680 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC024289.1
RefSeq Size	1630 bp
RefSeq ORF	1630 bp
Locus ID	390714
Domains	ig

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