FGD4 (NM_139241) Human Untagged Clone
CAT＃: SC128121
FGD4 (untagged)-Human FYVE, RhoGEF and PH domain containing 4 (FGD4)
CNY 5616.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CMT4H; FRABP; ZFYVE6 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC128121 sequence for NM_139241 edited (data generated by NextGen Sequencing)
ATGGAGGAAATTAAACCTGCCTCTGCTTCTTGTGTCTCAAAAGAAAAACCCAGTAAGGTA TCAGATCTCATCAGTCGCTTTGAAGGAGGCAGCTCATTATCAAATTATAGTGATTTGAAG AAAGAGTCTGCTGTGAACCTAAATGCTCCTAGAACCCCAGGAAGGCATGGATTGACAACC ACACCTCAACAAAAACTCCTCTCCCAGCACTTGCCACAGAGGCAGGGAAATGATACAGAT AAGACTCAGGGTGCACAGACTTGTGTGGCCAACGGTGTAATGGCAGCACAAAACCAGATG GAATGTGAGGAGGAGAAAGCTGCCACTCTTAGCTCAGATACTTCTATTCAAGCTTCTGAA CCCTTGCTTGATATGCACATAGTGAATGGAGAAAGAGATGAAACTGCCACAGCTCCTGCA TCACCCACAACAGACAGCTGTGATGGAAATGCTTCTGACAGTAGCTACAGGACTCCAGGC ATAGGCCCAGTGCTCCCCCTAGAAGAAAGAGGGGCAGAAACAGAAACCAAGGTACAAGAG AGGGAAAATGGGGAAAGCCCTCTGGAACTGGAGCAGCTGGACCAGCACCATGAGATGAAG GAGACTAATGAGCAAAAACTTCACAAAATAGCCAATGAACTTTTGCTTACTGAAAGAGCT TATGTCAACCGACTTGACCTCTTAGATCAGGTATTTTATTGCAAACTGTTGGAAGAAGCA AACCGAGGCTCGTTTCCAGCAGAGATGGTGAATAAAATCTTTTCTAATATTTCATCAATA AATGCCTTCCATAGTAAATTCCTCTTGCCAGAGCTGGAGAAACGAATGCAAGAATGGGAA ACTACTCCTAGAATTGGAGACATCCTTCAGAAATTGGCACCATTCCTTAAGATGTATGGA GAATATGTGAAAGGATTTGATAATGCAATGGAATTGGTTAAAAACATGACAGAACGTATT CCCCAGTTCAAATCAGTGGTTGAAGAAATTCAGAAACAGAAAATCTGTGGGAGCTTAACT TTGCAGCATCACATGCTAGAACCTGTTCAGCGGATTCCCCGGTATGAGATGCTCCTTAAG GACTATCTAAGGAAATTGCCTCCTGATTCCCTGGACTGGAATGATGCTAAAAAATCACTT GAAATTATATCTACAGCAGCAAGCCATTCTAATAGTGCAATAAGGAAAATGGAGAACCTA AAGAAACTCTTAGAGATTTATGAAATGTTGGGAGAAGAAGAAGACATTGTAAACCCTTCA AATGAACTAATAAAAGAAGGACAGATCCTCAAACTAGCTGCTCGAAACACTTCAGCACAA GAACGCTACCTTTTCTTATTCAACAACATGTTGCTGTACTGTGTGCCCAAATTCAGCTTG GTAGGCTCTAAATTCACAGTTCGAACCAGGGTTGGCATTGATGGAATGAAAATTGTAGAG ACTCAAAATGAAGAATATCCACATACTTTCCAGGTGTCTGGGAAAGAGAGAACACTGGAA CTGCAGGCCAGTTCTGCACAAGACAAAGAAGAATGGATCAAGGCCCTTCAAGAAACCATC GATGCTTTTCATCAAAGGCATGAAACCTTCAGAAATGCAATTGCAAAGGATAATGACATT CACTCAGAGGTTTCTACTGCTGAGCTAGGGAAAAGAGCCCCAAGATGGATCCGAGATAAT GAAGTGACAATGTGTATGAAATGTAAAGAACCTTTCAATGCACTGACACGAAGGAGGCAT CATTGTCGAGCATGTGGATATGTGGTTTGTTGGAAATGCTCCGACTACAAAGCTCAACTT GAATATGATGGTGGTAAATTGAGCAAAGTTTGTAAAGACTGTTATCAAATCATAAGTGGA TTCACAGACAGTGAAGAAAAGAAAAGAAAAGGAATTTTAGAGATTGAATCAGCAGAAGTA TCTGGAAACAGTGTGGTGTGCAGCTTTCTTCAGTATATGGAGAAGTCAAAACCTTGGCAG AAAGCTTGGTGTGTGATCCCCAAGCAAGACCCTCTTGTGCTGTACATGTATGGTGCCCCC CAGGACGTCAGAGCCCAGGCCACCATTCCACTTCTGGGCTATGTGGTGGATGAAATGCCA AGGAGCGCAGACCTGCCACACAGTTTCAAACTGACCCAGTCTAAGTCCGTGCACAGCTTT GCTGCAGACAGTGAGGAACTGAAGCAGAAGTGGCTGAAAGTCATCCTTTTAGCTGTCACA GGTGAGACACCAGGTGGTCCAAATGAGCATCCAGCCACCTTGGATGATCATCCTGAACCT AAGAAAAAATCAGAATGCTGA Clone variation with respect to NM_139241.2 374 c=>t;1305 g=>a;1518 g=>a >OriGene 5' read for NM_139241 unedited
GGGGTTCNCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCAGGGGA GCCTGTGCTGGGTGAGATTATCTGCAATGGACCGGGTTAATGGGAGGCTTGCTTTGGCTC GCTGGAAGAACAAATGAAGGGAGTTTTACTTCAACCATTTGTGACTTCTGGCCAAGAGGA ATAAATTCAGCTTTTATAAGGCAGCAGTACCTGTCCAAAGATCGCTTTAGTTCCACCTTG CTCCACAAGCAGCACAACCACACTGGTTGGTGAGAATGTATCTGAAGAAGAGGCTCAGGG AATAAATGGGAACAGGCCAGCAAAACACTCAGCTGCAAGTCCAAAGCCACAAGTGCCTCC AAAGCCATTACACCTGCAGAATTCACCTTCGTCCAATATACACCAAACCCCCAGGCATAA AGCTTTACCTAGTGCAAAACCAAGGATGGAGGAAATTAAACCTGCCTCTGCTTCTTGTGT CTCAAAAGAAAAACCCAGTAAGGTATCAGATCTCATCAGTCGCTTTGAAGGAGGCAGCTC ATTATCAAATTATAGTGATTTGAAGAAAGAGTCTGCTGTGAACCTAAATGCTCCTAGAAC CCCAGGAAGGCATGGATTGACAACCACACCTCAACAAAAACTCCTCTCCCAGCACTTGCC ACAGAGGCAGGGAAATGATACAGATAAGACTCAGGGTGCACAGACTTGTGTGGCCAACGG TGTAATGGCAGCACAAAACCAGATGGAATGTGAGGAGGAGAAAGCTGCCACTCTTAGCTC AGAACTTCTATTCAAGCTTCTGAACCCTTGCTTGATATGCACATAGTGAATGGAGAAAGA GATGAAACTGCCACAGCTCCTGCATCACCCACACAGACAGCTGTGATGGAAATGCTTCTG ACAGTAGCTACAGGACTTCCAGCTAC >OriGene 3' genomic read for NM_139241 unedited
GGGGCGGTTGTACATCTCAAAAAAGGTTTAATTTGNATAAAAAGCATAAGTTAAATAGTA AACTTAGTGTGAGATTTTAATCACAAGTACAAAAGAGTGAAGAGCAGCCTTCATGACAAG GAATCATGTGACCAGCCCCCACCCCAAACATAGGTTATCACATGTGGATTTTGTTTCATT CCGACAAAGGAAAAGGCCCATACAGTTATCTTAAGTGTTCATTATATAGAGACCTTATTT AACCATGCTCGATTATCTTACGGAAAAGAAAAACATGACGATAAATGTGGTTTCCACATA TCCTAGTTCTGTAAAAAGTCAAGTGTCTCATAAAGTTTGGGTTTTGGTGAAAATGTTTGT ACATGCTGATGTTAACTACTGCAATAATTATAGGATTGTTAACGGGTAAATAACCTGTAT GACTTTGACTGTCCCACTGACCAATATGCACTTTAATCTTACAATAATCAAGACTGACGC ATTAACTGAACCCTCATCTTATATTAATAGAACCTCTCTAGAGAGGCACTGTTTTAAATA GGCCCTGACACAAAAAATGGGCCTTTAAAAGTACTGTTCGGAATTCACAAATTAGTGCCA TGCCACATAAATTACCAATTTTTGTAAAAATATCAATCCCCTCCAATGCCAAATCGCAAA ACCTAAATCACCCGAAACATTTCTTACATTTCAGAACATTGCTCAGTTCCCACGGTACAC CTGGCTTTCATATTTAAAAATCAATTAGGGATTAATGTGGACCCTTACACCATGTCTAAC ATATATATTTAACTCCACATAAACCAAATTGCTATTTCCTTAAGTACATTCTTAAGCAGT CTTATTTGCAACACCATTATATTCCTTTCCTTTTTCAGTCGCGAAATACTAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_139241 |
| Insert Size | 4500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_139241.1, NP_640334.1 |
| RefSeq Size | 2931 bp |
| RefSeq ORF | 2301 bp |
| Locus ID | 121512 |
| UniProt ID | Q96M96 |
| Domains | RhoGEF, FYVE, PH |
| Gene Summary | This gene encodes a protein that is involved in the regulation of the actin cytoskeleton and cell shape. This protein contains an actin filament-binding domain, which together with its Dbl homology domain and one of its pleckstrin homology domains, can form microspikes. This protein can activate MAPK8 independently of the actin filament-binding domain, and it is also involved in the activation of CDC42 via the exchange of bound GDP for free GTP. The activation of CDC42 also enables this protein to play a role in mediating the cellular invasion of Cryptosporidium parvum, an intracellular parasite that infects the gastrointestinal tract. Mutations in this gene can cause Charcot-Marie-Tooth disease type 4H (CMT4H), a disorder of the peripheral nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015] Transcript Variant: This variant (3) has an alternate 5' terminal exon, which results in a different 5' UTR and translation initiation at a downstream AUG start codon, compared to variant 1. The resulting isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219442 | FGD4 (Myc-DDK-tagged)-Human FYVE, RhoGEF and PH domain containing 4 (FGD4) |
CNY 5608.00 |
|
| RC219442L3 | Lenti ORF clone of Human FYVE, RhoGEF and PH domain containing 4 (FGD4), Myc-DDK-tagged |
CNY 7700.00 |
|
| RC219442L4 | Lenti ORF clone of Human FYVE, RhoGEF and PH domain containing 4 (FGD4), mGFP tagged |
CNY 7700.00 |
|
| RG219442 | FGD4 (tGFP-tagged) - Human FYVE, RhoGEF and PH domain containing 4 (FGD4) |
CNY 7208.00 |
