DDI1 (NM_001001711) Human Untagged Clone

CAT#: SC300294

DDI1 (untagged)-Human DNA-damage inducible 1 homolog 1 (S. cerevisiae) (DDI1)



  "NM_001001711" in other vectors (4)

CNY 7220.00


货期*
4周

规格
    • 10 ug

Product images

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC300294 representing NM_001001711.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCTGATCACCGTGTACTGCGTGCGGAGGGACCTCTCCGAGGTCACCTTCTCTCTCCAGGTCAGCCCC
GACTTTGAGCTCCGAAACTTCAAGGTCCTCTGCGAAGCGGAGTCCAGAGTCCCCGTCGAAGAGATCCAG
ATCATCCACATGGAGCGACTCCTCATCGAGGACCACTGTTCCCTGGGCTCCTACGGCCTCAAAGATGGC
GATATCGTGGTTTTACTGCAGAAGGACAATGTGGGACCTCGGGCTCCAGGGCGTGCCCCGAACCAGCCT
CGTGTAGACTTCAGTGGCATTGCGGTGCCTGGGACGTCCAGCTCCCGTCCACAGCACCCTGGACAGCAG
CAGCAGCGCACACCCGCTGCCCAGCGGTCACAGGGCTTGGCGTCAGGAGAGAAGGTGGCCGGCCTGCAA
GGTCTGGGCAGCCCCGCCCTGATCCGCAGCATGCTGCTCTCCAACCCCCACGATCTGTCCCTGCTCAAG
GAACGCAACCCTCCCTTGGCGGAAGCCCTGCTCAGCGGAAGCCTTGAGACCTTTTCTCAGGTGCTGATG
GAGCAGCAAAGGGAAAAGGCCTTGAGAGAGCAAGAGAGGCTTCGTCTCTACACAGCCGACCCACTGGAT
CGGGAAGCTCAGGCCAAAATAGAAGAGGAAATCCGGCAGCAAAACATTGAAGAAAACATGAATATAGCG
ATAGAAGAGGCCCCCGAGAGTTTTGGACAAGTGACGATGCTCTACATTAACTGCAAAGTGAATGGGCAT
CCTTTGAAGGCTTTTGTTGACTCGGGCGCCCAGATGACCATTATGAGCCAGGCTTGTGCCGAGCGATGT
AACATCATGAGGCTGGTGGACCGACGGTGGGCTGGGGTTGCTAAAGGAGTGGGCACACAGAGAATTATT
GGCCGTGTTCATCTAGCTCAGATTCAAATTGAAGGTGATTTCTTACAGTGCTCTTTCTCCATACTTGAG
GATCAACCCATGGATATGCTTCTAGGCCTAGATATGCTCCGGAGACATCAATGTTCCATCGATTTGAAG
AAAAATGTGCTGGTCATCGGCACCACTGGCACGCAGACTTATTTTCTTCCTGAGGGAGAGTTGCCCTTA
TGCTCTAGGATGGTAAGTGGGCAAGATGAGTCTTCGGACAAGGAAATTACACATTCAGTCATGGATTCA
GGACGAAAAGAGCATTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001001711
Insert Size 1191 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001001711.2
RefSeq Size 2615 bp
RefSeq ORF 1191 bp
Locus ID 414301
UniProt ID Q8WTU0
MW 44.1 kDa
Gene Summary Probable aspartic protease (Probable). Seems to act as a proteasomal shuttle which links the proteasome and replication fork proteins like RTF2 (Probable). Required, with DDI2, for cellular survival following replication stress. Together or redudantly with DDI2, removes RTF2 from stalled forks to allow cell cycle progression after replication stress and maintains genome integrity (PubMed:29290612).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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