DDI1 (NM_001001711) Human Untagged Clone
CAT＃: SC300294
DDI1 (untagged)-Human DNA-damage inducible 1 homolog 1 (S. cerevisiae) (DDI1)
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC300294 representing NM_001001711.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTGATCACCGTGTACTGCGTGCGGAGGGACCTCTCCGAGGTCACCTTCTCTCTCCAGGTCAGCCCC GACTTTGAGCTCCGAAACTTCAAGGTCCTCTGCGAAGCGGAGTCCAGAGTCCCCGTCGAAGAGATCCAG ATCATCCACATGGAGCGACTCCTCATCGAGGACCACTGTTCCCTGGGCTCCTACGGCCTCAAAGATGGC GATATCGTGGTTTTACTGCAGAAGGACAATGTGGGACCTCGGGCTCCAGGGCGTGCCCCGAACCAGCCT CGTGTAGACTTCAGTGGCATTGCGGTGCCTGGGACGTCCAGCTCCCGTCCACAGCACCCTGGACAGCAG CAGCAGCGCACACCCGCTGCCCAGCGGTCACAGGGCTTGGCGTCAGGAGAGAAGGTGGCCGGCCTGCAA GGTCTGGGCAGCCCCGCCCTGATCCGCAGCATGCTGCTCTCCAACCCCCACGATCTGTCCCTGCTCAAG GAACGCAACCCTCCCTTGGCGGAAGCCCTGCTCAGCGGAAGCCTTGAGACCTTTTCTCAGGTGCTGATG GAGCAGCAAAGGGAAAAGGCCTTGAGAGAGCAAGAGAGGCTTCGTCTCTACACAGCCGACCCACTGGAT CGGGAAGCTCAGGCCAAAATAGAAGAGGAAATCCGGCAGCAAAACATTGAAGAAAACATGAATATAGCG ATAGAAGAGGCCCCCGAGAGTTTTGGACAAGTGACGATGCTCTACATTAACTGCAAAGTGAATGGGCAT CCTTTGAAGGCTTTTGTTGACTCGGGCGCCCAGATGACCATTATGAGCCAGGCTTGTGCCGAGCGATGT AACATCATGAGGCTGGTGGACCGACGGTGGGCTGGGGTTGCTAAAGGAGTGGGCACACAGAGAATTATT GGCCGTGTTCATCTAGCTCAGATTCAAATTGAAGGTGATTTCTTACAGTGCTCTTTCTCCATACTTGAG GATCAACCCATGGATATGCTTCTAGGCCTAGATATGCTCCGGAGACATCAATGTTCCATCGATTTGAAG AAAAATGTGCTGGTCATCGGCACCACTGGCACGCAGACTTATTTTCTTCCTGAGGGAGAGTTGCCCTTA TGCTCTAGGATGGTAAGTGGGCAAGATGAGTCTTCGGACAAGGAAATTACACATTCAGTCATGGATTCA GGACGAAAAGAGCATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001001711 |
| Insert Size | 1191 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001001711.2 |
| RefSeq Size | 2615 bp |
| RefSeq ORF | 1191 bp |
| Locus ID | 414301 |
| UniProt ID | Q8WTU0 |
| MW | 44.1 kDa |
| Gene Summary | Probable aspartic protease (Probable). Seems to act as a proteasomal shuttle which links the proteasome and replication fork proteins like RTF2 (Probable). Required, with DDI2, for cellular survival following replication stress. Together or redudantly with DDI2, removes RTF2 from stalled forks to allow cell cycle progression after replication stress and maintains genome integrity (PubMed:29290612).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205403 | DDI1 (Myc-DDK-tagged)-Human DNA-damage inducible 1 homolog 1 (S. cerevisiae) (DDI1) |
CNY 3656.00 |
|
| RC205403L3 | Lenti ORF clone of Human DNA-damage inducible 1 homolog 1 (S. cerevisiae) (DDI1), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC205403L4 | Lenti ORF clone of Human DNA-damage inducible 1 homolog 1 (S. cerevisiae) (DDI1), mGFP tagged |
CNY 5890.00 |
|
| RG205403 | DDI1 (tGFP-tagged) - Human DNA-damage inducible 1 homolog 1 (S. cerevisiae) (DDI1) |
CNY 5256.00 |
