TNRC6B (NM_001024843) Human Untagged Clone
CAT＃: SC302303
TNRC6B (untagged)-Human trinucleotide repeat containing 6B (TNRC6B), transcript variant 3
CNY 7176.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC302303 representing NM_001024843.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAAACCAATGAGGGAGAAGTATCGGAAGAAAGCAGTTCCAAGGTGGAACAGGAGGATTTTGTAATG GAAGGGCATGGCAAGACTCCACCTCCTGGTGAAGAAAGCAAACAAGAGAAGGAGCAAGAAAGGGAAGAA CAGTTAATGGAAGACAAGAAAAGGAAGAAAGAGGATAAAAAGAAAAAAGAAGCCACTCAGAAGGTCACG GAACAAAAAACCAAAGTGCCCGAAGTGACGAAACCAAGTTTAAGCCAACCAACGGCCGCCAGCCCAATT GGCAGCTCTCCATCGCCACCAGTCAATGGTGGCAACAATGCCAAAAGGGTGGCAGTGCCGAACGGACAA CCGCCAAGCGCCGCCCGCTACATGCCTCGGGAGGTGCCGCCGCGATTCCGTTGCCAGCAGGACCACAAA GTGTTACTAAAACGTGGGCAGCCCCCTCCACCGTCCTGCATGCTCCTTGGGGGTGGGGCAGGGCCTCCT CCCTGCACAGCACCTGGAGCAAACCCAAACAACGCACAAGTGACAGGAGCGCTGCTGCAGAGTGAGAGT GGGACTGCGCCAGTCTGGAGCAAAAGCACACCACCTGCTCCAGATAATGGTACTTCCGCTTGGGGTGAG CCAAATGAAAGCAGTCCTGGGTGGGGCGAGATGGATGATACGGGAGCATCGACCACAGGCTGGGGGAAC ACGCCCGCCAACGCTCCCAATGCCATGAAGCCTAATTCCAAATCTATGCAAGACGGCTGGGGGGAGAGT GACGGGCCAGTCACAGGAGCTCGCCATCCCAGCTGGGAAGAGGAGGAGGATGGAGGAGTCTGGAACACC ACTGGCTCTCAGGGCAGTGCTTCCTCCCACAACTCAGCAAGCTGGGGACAAGGAGGAAAGAAACAAATG AAGTGCTCACTCAAAGGAGGAAACAATGATTCATGGATGAATCCTCTTGCCAAACAGTTTTCAAATATG GGATTGCTGAGTCAGACTGAAGATAATCCAAGCAGCAAAATGGATTTGTCTGTAGGAAGCCTTTCAGAT AAAAAATTTGATGTGGACAAGCGAGCGATGAATCTCGGGGATTTTAATGATATCATGAGGAAGGATCGA TCTGGGTTCCGTCCACCTAATTCCAAAGACATGGGAACCACAGATAGTGGGCCTTATTTTGAGAAGGGC GGTAGTCATGGTTTGTTTGGAAACAGCACAGCACAATCGAGAGGTCTGCACACACCCGTGCAGCCACTA AATTCTTCTCCCAGTCTCCGGGCGCAAGTGCCTCCCCAGTTTATTTCCCCCCAGGTTTCTGCCTCAATG CTCAAGCAGTTTCCCAACAGTGGCCTGAGTCCAGGTCTTTTCAATGTGGGGCCCCAGTTATCTCCTCAA CAAATTGCCATGCTGAGCCAGCTTCCACAAATTCCCCAGTTTCAGTTGGCATGTCAGCTTCTCTTGCAG CAGCAGCAACAGCAGCAGTTGTTACAGAACCAGAGAAAGATTTCTCAAGCTGTACGCCAACAGCAAGAG CAGCAGCTGGCTCGAATGGTGAGTGCACTGCAGCAGCAGCAGCAGCAGCAGCAGAGGCAGCCAGGCATG AAGCACTCGCCCTCTCATCCTGTTGGGCCCAAGCCGCATCTGGACAACATGGTACCCAACGCATTGAAT GTGGGGCTCCCAGACCTTCAAACCAAAGGGCCAATACCTGGATATGGTTCTGGCTTCAGCTCTGGCGGC ATGGACTATGGCATGGTTGGTGGGAAGGAGGCTGGAACCGAGTCTCGCTTTAAACAGTGGACCTCCATG ATGGAGGGGCTGCCCTCTGTAGCCACACAGGAAGCCAATATGCACAAAAATGGCGCTATAGTGGCCCCT GGTAAAACCCGGGGAGGGTCACCGTACAACCAGTTTGATATCATCCCTGGTGACACACTGGGTGGCCAT ACGGGTCCTGCTGGTGATAGCTGGTTACCTGCCAAATCTCCACCAACAAATAAAATCGGAAGTAAATCC AGCAATGCCAGTTGGCCTCCAGAATTCCAACCAGGAGTGCCATGGAAAGGTATCCAAAACATTGACCCT GAATCTGACCCCTATGTCACCCCAGGAAGTGTGCTGGGGGGTACAGCCACATCTCCCATTGTAGATACT GACCACCAACTGCTGCGGGATAACACCACAGGGTCTAATTCTTCCCTCAACACCTCGCTGCCTTCACCT GGTGCCTGGCCCTACAGTGCCTCTGACAACTCCTTTACCAACGTTCATAGCACTTCAGCAAAGTTCCCT GATTACAAATCAACATGGTCCCCAGATCCCATAGGACACAACCCCACTCATCTCTCCAACAAGATGTGG AAAAACCATATTTCCTCCAGGAACACTACACCGCTGCCCCGCCCACCTCCTGGTCTGACCAACCCCAAA CCATCATCTCCCTGGAGCAGCACAGCACCCCGATCAGTCAGGGGGTGGGGGACACAGGACTCACGGCTC GCCTCGGCCTCTACCTGGAGTGATGGTGGCTCAGTTCGTCCTAGTTACTGGCTGGTTCTTCACAATCTC ACCCCACAGATTGATGGGTCAACCTTGAGAACGATCTGCATGCAGCATGGCCCACTGCTGACATTCCAT CTGAATCTAACCCAGGGCACTGCCCTGATCCGATACAGCACCAAACAGGAGGCGGCCAAGGCCCAAACT GCACTGCACATGTGTGTGTTGGGAAACACTACCATCCTTGCTGAGTTTGCCACTGATGATGAAGTCAGC CGCTTTCTGGCACAAGCTCAGCCCCCTACACCTGCAGCAACCCCAAGTGCGCCAGCTGCGGGGTGGCAG TCGCTGGAGACCGGCCAGAACCAGTCAGATCCCGTGGGACCTGCTCTGAATCTTTTTGGTGGGTCCACT GGGCTCGGGCAGTGGAGCAGCAGCGCTGGTGGCAGCAGCGGGGCCGATCTTGCTGGCGCTTCATTGTGG GGGCCCCCAAACTATTCTTCTAGCTTATGGGGAGTCCCAACGGTGGAAGATCCCCATAGGATGGGCAGC CCTGCTCCTTTACTACCTGGTGACCTTCTGGGAGGAGGGTCGGATTCAATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001024843 |
| Insert Size | 3090 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001024843.1 |
| RefSeq Size | 15976 bp |
| RefSeq ORF | 3090 bp |
| Locus ID | 23112 |
| UniProt ID | Q9UPQ9 |
| MW | 109.3 kDa |
| Gene Summary | Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent translational repression and siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. As scaffoldng protein associates with argonaute proteins bound to partially complementary mRNAs and simultaneously can recruit CCR4-NOT and PAN deadenylase complexes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate promoter, has a different translation start site, and is missing two in-frame coding exons compared to variant 1. This results in a shorter isoform (3) with a distinct N-terminus and lacking two internal protein segments compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206220 | TNRC6B (Myc-DDK-tagged)-Human trinucleotide repeat containing 6B (TNRC6B), transcript variant 3 |
CNY 7168.00 |
|
| RC206220L3 | Lenti ORF clone of Human trinucleotide repeat containing 6B (TNRC6B), transcript variant 3, Myc-DDK-tagged |
CNY 10740.00 |
|
| RC206220L4 | Lenti ORF clone of Human trinucleotide repeat containing 6B (TNRC6B), transcript variant 3, mGFP tagged |
CNY 10740.00 |
|
| RG206220 | TNRC6B (tGFP-tagged) - Human trinucleotide repeat containing 6B (TNRC6B), transcript variant 3 |
CNY 9690.00 |
