Melanopsin (OPN4) (NM_001030015) Human Untagged Clone
CAT＃: SC302494
OPN4 (untagged)-Human opsin 4 (OPN4), transcript variant 2
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MOP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC302494 representing NM_001030015.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAACCCTCCTTCGGGGCCAAGAGTCCCGCCCAGCCCAACCCAAGAGCCCAGCTGCATGGCCACCCCA GCACCACCCAGCTGGTGGGACAGCTCCCAGAGCAGCATCTCCAGCCTGGGCCGGCTTCCATCCATCAGT CCCACAGCACCTGGGACTTGGGCTGCTGCCTGGGTCCCCCTCCCCACGGTTGATGTTCCAGACCATGCC CACTATACCCTGGGCACAGTGATCTTGCTGGTGGGACTCACGGGGATGCTGGGCAACCTGACGGTCATC TATACCTTCTGCAGAGCTGTGCTTCGTGGAGTCACTGTGATGATGCAGAGCAGAAGCCTCCGGACACCT GCCAACATGTTCATTATCAACCTCGCGGTCAGCGACTTCCTCATGTCCTTCACCCAGGCCCCTGTCTTC TTCACCAGTAGCCTCTATAAGCAGTGGCTCTTTGGGGAGACAGGCTGCGAGTTCTATGCCTTCTGTGGA GCTCTCTTTGGCATTTCCTCCATGATCACCCTGACGGCCATCGCCCTGGACCGCTACCTGGTAATCACA CGCCCGCTGGCCACCTTTGGTGTGGCGTCCAAGAGGCGTGCGGCATTTGTCCTGCTGGGCGTTTGGCTC TATGCCCTGGCCTGGAGTCTGCCACCCTTCTTCGGCTGGAGCGCCTACGTGCCCGAGGGGTTGCTGACA TCCTGCTCCTGGGACTACATGAGCTTCACGCCGGCCGTGCGTGCCTACACCATGCTTCTCTGCTGCTTC GTGTTCTTCCTCCCTCTGCTTATCATCATCTACTGCTACATCTTCATCTTCAGGGCCATCCGGGAGACA GGACGGGCTCTCCAGACCTTCGGGGCCTGCAAGGGCAATGGCGAGTCCCTGTGGCAGCGGCAGCGGCTG CAGAGCGAGTGCAAGATGGCCAAGATCATGCTGCTGGTCATCCTCCTCTTCGTGCTCTCCTGGGCTCCC TATTCCGCTGTGGCCCTGGTGGCCTTTGCTGGGTACGCACACGTCCTGACACCCTACATGAGCTCGGTG CCAGCCGTCATCGCCAAGGCCTCTGCAATCCACAACCCCATCATTTACGCCATCACCCACCCCAAGTAC AGGGTGGCCATTGCCCAGCACCTGCCCTGCCTGGGGGTGCTGCTGGGTGTATCACGCCGGCACAGTCGC CCCTACCCCAGCTACCGCTCCACCCACCGCTCCACGCTGACCAGCCACACCTCCAACCTCAGCTGGATC TCCATACGGAGGCGCCAGGAGTCCCTGGGCTCGGAGAGTGAGGTGGGCTGGACACACATGGAGGCAGCA GCTGTGTGGGGAGCTGCCCAGCAAGCAAATGGGCGGTCCCTCTACGGTCAGGGTCTGGAGGACTTGGAA GCCAAGGCACCCCCCAGACCCCAGGGACACGAAGCAGAGACTCCAGGGAAGACCAAGGGGCTGATCCCC AGCCAGGACCCCAGGATGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001030015 |
| Insert Size | 1470 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001030015.2 |
| RefSeq Size | 2419 bp |
| RefSeq ORF | 1470 bp |
| Locus ID | 94233 |
| UniProt ID | Q9UHM6 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 53.8 kDa |
| Gene Summary | Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This gene encodes a photoreceptive opsin protein that is expressed within the ganglion and amacrine cell layers of the retina. In mouse, retinal ganglion cell axons expressing this gene projected to the suprachiasmatic nucleus and other brain nuclei involved in circadian photoentrainment. In mouse, this protein is coupled to a transient receptor potential (TRP) ion channel through a G protein signaling pathway and produces a physiologic light response via membrane depolarization and increased intracellular calcium. The protein functions as a sensory photopigment and may also have photoisomerase activity. Experiments with knockout mice indicate that this gene attenuates, but does not abolish, photoentrainment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) includes an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in a longer protein (isoform 2) compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215793 | OPN4 (Myc-DDK-tagged)-Human opsin 4 (OPN4), transcript variant 2 |
CNY 3656.00 |
|
| RC215793L3 | Lenti-ORF clone of OPN4 (Myc-DDK-tagged)-Human opsin 4 (OPN4), transcript variant 2 |
CNY 5890.00 |
|
| RC215793L4 | Lenti-ORF clone of OPN4 (mGFP-tagged)-Human opsin 4 (OPN4), transcript variant 2 |
CNY 5890.00 |
|
| RG215793 | OPN4 (tGFP-tagged) - Human opsin 4 (OPN4), transcript variant 2 |
CNY 4370.00 |
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