RGL3 (NM_001035223) Human Untagged Clone
CAT＃: SC302825
RGL3 (untagged)-Human ral guanine nucleotide dissociation stimulator-like 3 (RGL3), transcript variant 2
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC302825 representing NM_001035223.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCGCACAGCAGGCAAAGAGCTGGCCCTGGCACCGCTGCAGGACTGGGGTGAAGAGACCGAGGAC GGCGCGGTGTACAGTGTCTCCCTGCGGCGGCAGCGCAGTCAGCGCAGGAGCCCGGCGGAGGGCCCCGGG GGCAGCCAGGCTCCCAGCCCCATTGCCAATACCTTCCTCCACTATCGAACCAGCAAGGTGAGGGTGCTG AGGGCAGCGCGCCTGGAGCGGCTGGTGGGAGAGTTGGTGTTTGGAGACCGTGAGCAGGACCCCAGCTTC ATGCCCGCCTTCCTGGCCACCTACCGGACCTTTGTACCCACTGCCTGCCTGCTGGGCTTTCTGCTGCCA CCAATGCCACCGCCCCCACCTCCCGGGGTAGAGATCAAGAAGACAGCGGTACAAGATCTGAGCTTCAAC AAGAACCTGAGGGCTGTGGTGTCAGTGCTGGGCTCCTGGCTGCAGGACCACCCTCAGGATTTCCGAGAC CACCCTGCCCATTCGGACCTGGGCAGTGTCCGAACCTTTCTGGGCTGGGCGGCCCCAGGGAGTGCTGAG GCTCAAAAAGCAGAGAAGCTTCTGGAAGATTTTTTGGAGGAGGCTGAGCGAGAGCAGGAAGAGGAGCCG CCTCAGGTGTGGACAGGACCTCCCAGAGTTGCCCAAACTTCTGACCCAGACTCTTCAGAGGCCTGCGCG GAGGAAGAGGAAGGGCTCATGCCTCAAGGTCCCCAGCTCCTGGACTTCAGCGTGGACGAGGTGGCCGAG CAGCTGACCCTCATAGACTTGGAGCTCTTCTCCAAGGTGAGGCTCTACGAGTGCTTGGGCTCCGTGTGG TCGCAGAGGGACCGGCCGGGGGCTGCAGGCGCCTCCCCCACTGTGCGCGCCACCGTGGCCCAGTTCAAC ACCGTGACCGGCTGTGTGCTGGGTTCCGTGCTCGGAGCACCGGGCTTGGCCGCCCCGCAGAGGGCGCAG CGGCTGGAGAAGTGGATCCGCATCGCCCAGCGCTGCCGAGAACTGCGGAACTTCTCCTCCTTGCGCGCC ATCCTGTCCGCCCTGCAATCTAACCCCATCTACCGGCTCAAGCGCAGCTGGGGGGCAGTGAGCCGGGAA CCGCTATCTACTTTCAGGAAACTTTCGCAGATTTTCTCCGATGAGAACAACCACCTCAGCAGCAGAGAG ATTCTTTTCCAGGAGGAGGCCACTGAGGGATCCCAAGAAGAGGACAACACCCCAGGCAGCCTGCCCTCA AAACCACCCCCAGGCCCTGTCCCCTACCTTGGCACCTTCCTTACGGACCTGGTTATGCTGGACACAGCC CTGCCGGATATGTTGGAGGGGGATCTCATTAACTTTGAGAAGAGGAGGAAGGAGTGGGAGATCCTGGCC CGCATCCAGCAGCTGCAGAGGCGCTGTCAGAGCTACACCCTGAGCCCCCACCCGCCCATCCTGGCTGCC CTGCATGCCCAGAACCAGCTCACCGAGGAGCAGAGCTACCGGCTCTCCCGGGTCATTGAGCCACCAGCT GCCTCCTGCCCCAGCTCCCCACGCATCCGACGGCGGATCAGCCTCACCAAGCGTCTCAGTGCGAAGCTT GCCCGAGAGAAAAGCTCATCACCTAGTGGGAGTCCCGGGGACCCCTCATCCCCCACCTCCAGTGTGTCC CCAGGGTCACCCCCCTCAAGTCCTAGAAGCAGAGATGCTCCTGCTGGCAGTCCCCCGGCCTCTCCAGGG CCCCAGGGCCCCAGCACCAAGCTGCCCCTGAGCCTGGACCTGCCCAGCCCCCGGCCCTTCGCTTTGCCT CTGGGCAGCCCTCGAATCCCCCTCCCGGCGCAGCAGAGCTCGGAGGCCCGTGTCATCCGCGTCAGCATC GACAATGACCACGGGAACCTGTATCGAAGCATCTTGCTGACCAGTCAGGACAAAGCCCCCAGCGTGGTC CGGCGAGCCTTGCAGAAGCACAATGTGCCCCAGCCCTGGGCCTGTGACTATCAGCTCTTTCAAGTCCTT CCTGGGGACCGGGTGCTCCTGATTCCTGACAATGCCAACGTCTTCTATGCCATGAGTCCAGTCGCCCCC AGAGACTTCATGCTGCGGCGGAAAGAGGGGACCCGGAACACTCTGTCTGTCTCCCCAAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001035223 |
| Insert Size | 2133 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001035223.3 |
| RefSeq Size | 2553 bp |
| RefSeq ORF | 2133 bp |
| Locus ID | 57139 |
| UniProt ID | Q3MIN7 |
| MW | 78.1 kDa |
| Gene Summary | Guanine nucleotide exchange factor (GEF) for Ral-A. Potential effector of GTPase HRas and Ras-related protein M-Ras. Negatively regulates Elk-1-dependent gene induction downstream of HRas and MEKK1 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211012 | RGL3 (Myc-DDK-tagged)-Human ral guanine nucleotide dissociation stimulator-like 3 (RGL3), transcript variant 2 |
CNY 5200.00 |
|
| RC211012L3 | Lenti ORF clone of Human ral guanine nucleotide dissociation stimulator-like 3 (RGL3), transcript variant 2, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC211012L4 | Lenti ORF clone of Human ral guanine nucleotide dissociation stimulator-like 3 (RGL3), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RG211012 | RGL3 (tGFP-tagged) - Human ral guanine nucleotide dissociation stimulator-like 3 (RGL3), transcript variant 2 |
CNY 4370.00 |
