LIPF (NM_004190) Human Untagged Clone
CAT＃: SC303449
LIPF (untagged)-Human lipase, gastric (LIPF), transcript variant 2
CNY 3656.00
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GL; HGL; HLAL |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_004190, RT-PCR generated
AAATGTGGCTGCTTTTAACAATGGCAAGTTTGATATCTGTACTGGGGACTACACATGGTT TGTTTGGAAAATTACATCCTGGAAGCCCTGAAGTGACTATGAACATTAGTCAGATGATTA CTTATTGGGGATACCCAAATGAAGAATATGAAGTTGTGACTGAAGATGGTTATATTCTTG AAGTCAATAGAATTCCTTATGGGAAGAAAAATTCAGGGAATACAGGCCAGAGACCTGTTG TGTTTTTGCAGCATGGTTTGCTTGCATCAGCCACAAACTGGATTTCCAACCTGCCGAACA ACAGCCTTGCCTTCATTCTGGCAGATGCTGGTTATGATGTGTGGCTGGGCAACAGCAGAG GAAACACCTGGGCCAGAAGAAACTTGTACTATTCACCAGATTCAGTTGAATTCTGGGCTT TCAGCTTTGATGAAATGGCTAAATATGACCTTCCAGCCACAATCGACTTCATTGTAAAGA AAACTGGACAGAAGCAGCTACACTATGTTGGCCATTCCCAGGGCACCACCATTGGTTTTA TTGCCTTTTCCACCAATCCCAGCCTGGCTAAAAGAATCAAAACCTTCTATGCTCTAGCTC CTGTTGCCACTGTGAAGTATACAAAAAGCCTTATAAACAAACTTAGATTTGTTCCTCAAT CCCTCTTCAAGTTTATATTTGGTGACAAAATATTCTACCCACACAACTTCTTTGATCAAT TTCTTGCTACTGAAGTGTGCTCCCGTGAGATGCTGAATCTCCTTTGCAGCAATGCCTTAT TTATAATTTGTGGATTTGACAGTAAGAACTTTAACACGAGTCGCTTGGATGTGTATCTAT CACATAATCCAGCAGGAACTTCTGTTCAAAACATGTTCCATTGGACCCAGGCTGTTAAGT CTGGGAAATTCCAAGCTTATGACTGGGGAAGCCCAGTTCAGAATAGGATGCACTATGATC AGTCCCAACCTCCCTACTACAATGTGACAGCCATGAATGTACCAATTGCAGTGTGGAACG GTGGCAAGGACCTGTTGGCTGACCCCCAAGATGTTGGCCTTTTGCTTCCAAAACTCCCCA ATCTTATTTACCACAAGGAGATTCCTTTTTACAATCACTTGGACTTTATCTGGGCAATGG ATGCCCCTCAAGAAGTTTACAATGACATTGTTTCTATGATATCAGAAGATAAAAAGTAGT TCTGGATTTAAAGAATTATCCGTTTGTTTTT >OriGene 5' read for NM_004190 unedited
NNNNNNAAGTTCACTATTGTATACGACTCCTATAGGCGGCCGCGNATTCAGATCTGGTAC CAAATGTGGCTGCTTTTAACAATGGCAAGTTTGATATCTGTACTGGGGACTACACATGGT TTGTTTGGAAAATTACATCCTGGAAGCCCTGAAGTGACTATGAACATTAGTCAGATGATT ACTTATTGGGGATACCCAAATGAAGAATATGAAGTTGTGACTGAAGATGGTTATATTCTT GAAGTCAATAGAATTCCTTATGGGAAGAAAAATTCAGGGAATACAGGCCAGAGACCTGTT GTGTTTTTGCAGCATGGTTTGCTTGCATCAGCCACAAACTGGATTTCCAACCTGCCGAAC AACAGCCTTGCCTTCATTCTGGCAGATGCTGGTTATGATGTGTGGCTGGGCAACAGCAGA GGAAACACCTGGGCCAGAAGAAACTTGTACTATTCACCAGATTCAGTTGAATTCTGGGCT TTCAGCTTTGATGAAATGGCTAAATATGACCTTCCAGCCACAATCGACTTCATTGTAAAG AAAACTGGACAGAAGCAGCTACACTATGTTGGCCATTCCCAGGGCACCACCATTGGGTTT TATTGCCTTTTCCACCAATCCCAGCCTGGCTAAAAGAATCAAAACCTTCTATGCTCTAGC TCCTGTTGCCACTGTGAAGTATACAAAAAGCCTTATAAACAAACTTAGNATTTGTTCCTC AATCCCTCTTCAAGTTTATTATTTGGTGACAAAATATTCTACCCACACAACTTCTTTGAT CAATTTCTTGCTACTGGAAGTGTGCTCCCGTGAGATGCTGAATCTCCTTTGCAGCAATGC CTTATTTATAATTTGGTGGATTTGACAGTAAGAACTTTAACACGAGTCGTTTGGGGATGT GTAT >OriGene 3' read for NM_004190 unedited
NNCCATTGGNGAATGGCACTTNCCAGGNCCAGNANAGCACTGGGGNAGGGTCACAGGNAT GCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTT GNGAAAAACAAACGGATAATTCTTTAAATCCAGAACTACTTTTTATCTTCTGATATCATA GAAACAATGTCATTGTAAACTTCTTGAGGGGCATCCATTGCCCAGATAAAGTCCAAGTGA TTGTAAAAAGGAATCTCCTTGTGGTAAATAAGATTGGGGAGTTTTGGAAGCAAAAGGCCA ACATCTTGGGGGTCAGCCAACAGGTCCTTGCCACCGTTCCACACTGCAATTGGTACATTC ATGGCTGTCACATTGTAGTAGGGAGGTTGGGACTGATCATAGTGCATCCTATTCTGAACT GGGCTTCCCCAGTCATAAGCTTGGAATTTCCCAGACTTAACAGCCTGNGTCCAATGGAAC ATGTTTTGAACAGAAGTTCCTGCTGGATTATGTGATAGATACACATCCAAGCGACTCGTG TTAAAGTTCTTACTGTCAAATCCACAAATTATAAATAAGGCATTGCTGCANAGGAGATTC AGCATCTCACGGGAGCACACTTCAGTAGCAAGAAATTGATCAAAGAAAAGTTGTGTGGGT AGAAATATTTTGTCACCAAATATAAACTTGAAGAGGGGATTGAGGAACANATCTAAAGGT TTGTTAATAAGGCTTTTTGTATACTTCACAGTGGCAACAGGAGCTAGACCTAANAAAGGT TTTGATTCTTTTTACCAGGCTGGGATTGGGGGGAAAAGGCAATAAACCAATGGTTGGTGC CCTGGGAATGGNCCACATAGTGTAACTGGCTCTGTCCCGGTTTCTTACAATGAAATCGAT GTGGCTGGGG |
| Restriction Sites | Please inquire |
| ACCN | NM_004190 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This construct is a cloned PCR fragment that was fully sequenced. There are no nucleotide differences between the OriGene clone and the NCBI reference ORF. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004190.1, NP_004181.1 |
| RefSeq Size | 1365 bp |
| RefSeq ORF | 1197 bp |
| Locus ID | 8513 |
| UniProt ID | P07098 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Glycerolipid metabolism, Metabolic pathways |
| Gene Summary | This gene encodes gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221225 | LIPF (Myc-DDK-tagged)-Human lipase, gastric (LIPF), transcript variant 2 |
CNY 3656.00 |
|
| RC221225L1 | Lenti ORF clone of Human lipase, gastric (LIPF), transcript variant 2, Myc-DDK-tagged |
CNY 6056.00 |
|
| RC221225L2 | Lenti ORF clone of Human lipase, gastric (LIPF), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RC221225L3 | Lenti ORF clone of Human lipase, gastric (LIPF), transcript variant 2, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC221225L4 | Lenti ORF clone of Human lipase, gastric (LIPF), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RG221225 | LIPF (tGFP-tagged) - Human lipase, gastric (LIPF), transcript variant 2 |
CNY 5256.00 |
