DLEC1 (NM_007337) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CFAP81; DLC-1; DLC1; F56; FAP81
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC303915 representing NM_007337. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGACCAGGAGCTCCAAAACGCGGAGGTCTTTAGCGTCCCGGACCAACGAGTGCCAGGGGACAATG TGGGCGCCAACTTCGCCACCAGCCGGGTCCAGCAGCCCCAGCCAGCCCACCTGGAAGTCCTCCTTGTAT TCCTCCCTCGCCTACTCTGAGGCCTTCCACTACAGCTTCGCAGCCCGGCCCCGCCGCCTCACGCAGCTT GCGCTGGCGCAGCGTCCCGAGCCTCAGCTGCTTCGTCTGCGCCCCTCCTCGCTGCGCACCCAAGATATC TCGCACTTGCTCACCGGCGTCTTCCGCAACTTGTACTCAGCCGAGGTCATCGGCGACGAAGTGAGCGCA AGCTTGATCAAGGCCCGCGGCAGCGAGAATGAGCGCCACGAGGAGTTCGTGGACCAGCTGCAGCAGATT CGGGAGCTCTATAAGCAGCGGCTGGATGAGTTTGAAATGTTGGAGAGACATATCACTCAGGCCCAAGCA CGGGCTATTGCGGAAAATGAGCGGGTCATGAGCCAGGCTGGAGTACAGGACCTCGAGAGCCTTGTCAGG TTGCCTCCAGTGAAGAGTGTCTCCAGATGGTGTATAGACAGCGAGTTGCTACGGAAACATCATTTGATC TCCCCAGAAGATTACTACACCGATACAGTGCCGTTTCACTCTGCACCTAAAGGCATCTCCCTACCTGGA TGTTCAAAACTGACATTTAGCTGTGAGAAGCGTTCCGTCCAGAAGAAAGAGCTGAACAAGAAGCTTGAA GATTCATGCAGGAAGAAGCTTGCTGAGTTCGAAGATGAGTTAGACCACACTGTGGACAGCCTGACATGG AATTTAACTCCTAAGGCCAAAGAAAGGACCAGAGAACCTCTCAAGAAAGCAAGTCAACCAAGGAATAAA AACTGGATGAACCACTTACGTGTGCCACAGAGAGAGCTAGACAGACTTCTGCTTGCCAGAATGGAGAGT CGGAACCACTTCCTAAAAAATCCCCGTTTTTTTCCTCCTAACACTCGATATGGAGGCAAGTCTCTTGTT TTTCCTCCAAAGAAGCCAGCACCGATAGGAGAATTCCAGAGTACAGAGCCAGAACAGAGTTGTGCTGAT ACTCCAGTGTTTCTAGCTAAGCCACCAATTGGGTTTTTCACAGATTATGAAATTGGTCCAGTTTATGAG ATGGTAATTGCGCTGCAGAACACCACCACGACCAGCCGCTACCTGCGAGTCCTCCCGCCTTCCACGCCA TACTTCGCTCTGGGACTGGGGATGTTCCCAGGAAAAGGTGGAATGGTGGCTCCTGGAATGACCTGCCAG TACATTGTCCAGTTTTTTCCCGACTGCCTTGGGGATTTTGATGATTTTATTTTAGTGGAGACCCAGTCA GCCCACACACTTCTGATCCCCCTGCAGGCCCGGAGGCCGCCCCCCGTGCTGACATTGTCACCGGTGTTG GACTGTGGTTACTGCCTCATTGGGGGAGTCAAGATGACCAGATTCATCTGCAAAAATGTGGGTTTCAGT GTTGGCAGGTTCTGCATTATGCCCAAAACAAGCTGGCCACCACTAAGTTTCAAGGCCATTGCAACCGTC GGCTTTGTTGAACAACCTCCTTTTGGAATCCTGCCTTCGGTGTTTGAGCTGGCCCCGGGACATGCTATA TTAGTGGAGGTCTTGTTTTCCCCAAAGAGCCTAGGAAAGGCAGAGCAGACCTTCATCATCATGTGCGAC AACTGCCAGATAAAGGAGCTGGTGACCATAGGAATTGGGCAGCTGATTGCTTTGGATCTGATCTATATT TCTGGTGAAAAAAGCCAGCCAGACCCTGGAGAGCTCACAGACTTAACAGCCCAGCACTTCATACGATTT GAGCCTGAAAACCTTCGGTCCACGGCTAGGAAGCAGCTGATTATTAGAAATGCTACGCACGTGGAGCTG GCCTTCTACTGGCAGATCATGAAGCCCAACCTGCAGCCCCTCATGCCTGGAGAAACCTTCAGCATGGAC AGCATCAAGTGCTACCCCGACAAGGAGACTGCCTTCTCCATCATGCCCAGAAAGGGGGTTCTAAGCCCC CACACAGACCACGAGTTCATCCTGAGCTTTTCTCCTCATGAGCTGAGGGATTTTCACAGTGTGCTCCAG ATGGTGCTAGAGGAAGTCCCAGAGCCTGTAAGTTCAGAAGCGGAGAGCCTGGGGCACTCCTCCTACTCT GTGGATGATGTGATTGTCCTGGAAATCGAGGTGAAAGGCTCAGTAGAACCTTTCCAGGTTCTCTTAGAG CCATATGCCCTCATCATCCCAGGGGAGAACTACATTGGGATAAATGTGAAGAAGGCTTTTAAGATGTGG AACAACAGCAAGTCACCCATCAGATACCTGTGGGGGAAGATCAGCGACTGCCACATCATTGAAGTGGAG CCCGGCACAGGGGTCATAGAGCCCAGTGAGGTCGGGGATTTTGAGTTGAACTTTACTGGGGGTGTCCCT GGCCCCACAAGCCAGGACCTGCTGTGTGAAATCGAAGACTCGCCCTCGCCAGTGGTGTTACACATTGAG GCTGTCTTTAAGGGGCCTGCCCTCATCATCAACGTCTCAGCCCTTCAGTTTGGTCTGCTCCGCCTGGGG CAGAAAGCCACAAACTCCATCCAGATCCGGAACGTCAGCCAGCTCCCAGCCACATGGCGCATGAAGGAG AGCCCAGTCTCCCTCCAGGAAAGGCCTGAGGATGTGTCTCCCTTCGACATTGAGCCTTCGAGTGGCCAG CTTCACTCTCTGGGGGAGTGCAGGGTGGACATCACCTTGGAGGCCCTGCACTGCCAGCATCTGGAGACC GTCCTGGAGCTGGAGGTGGAAAATGGTGCCTGGAGCTACCTTCCTGTGTATGCTGAGGTACAGAAGCCC CATGTGTACCTACAGAGCAGCCAGGTGGAGGTTAGAAATCTCTACCTGGGTGTGCCCACGAAGACAACC ATCACACTTATCAATGGCACGCTCCTGCCTACCCAGTTCCACTGGGGCAAGCTCCTCGGACACCAAGCA GAATTCTGCATGGTGACAGTCTCCCCCAAACATGGCCTGCTGGGCCCAAGTGAGGAGTGCCAGCTCAAG TTGGAGTTGACTGCTCATACCCAGGAAGAGCTGACCCATCTGGCCCTCCCTTGTCACGTGTCAGGCATG AAGAAGCCACTGGTTCTAGGCATTTCTGGGAAGCCCCAGGGACTGCAAGTGGCCATTACCATCTCTAAG GAGAGCTCTGATTGCAGCACAGAGCAGTGGCCAGGCCACCCAAAGGAGCTCCGCCTGGACTTTGGCTCA GCGGTGCCACTGAGGACCCGTGTGACTCGCCAGCTCATTCTCACCAATCGCTCCCCAATACGGACCCGT TTCTCCCTCAAGTTTGAGTATTTCGGGAGCCCCCAAAACAGCCTGAGCAAAAAGACCAGCCTTCCCAAC ATGCCTCCTGCCCTGCTAAAGACAGTGCGGATGCAAGAGCACCTGGCCAAGCGAGAGCAGCTGGATTTT ATGGAGAGCATGCTATCCCACGGGAAAGGAGCTGCTTTCTTCCCTCACTTTTCCCAGGGCATGCTGGGG CCCTACCAGCAGCTGTGCATTGACATCACAGGCTGTGCCAACATGTGGGGCGAGTACTGGGACAACCTC ATCTGCACGGTGGGAGACCTGCTGCCGGAAGTCATCCCAGTGCACATGGCAGCGGTGGGCTGCCCCATC AGCTCCCTGAGGACCACCTCCTACACTATTGACCAGGCCCAGAAGGAACCAGCCATGAGGTTCGGCACC CAGGTCTCCGGAGGAGACACAGTTACCCGAACCCTTCGCCTGAATAACTCCAGCCCCTGTGACATCCGC CTGGATTGGGAGACCTATGTTCCAGAAGACAAGGAAGACCGGCTGGTGGAGCTGCTGGTGTTTTATGGG CCACCTTTCCCGCTGCGGGACCAAGCCGGGAATGAGCTTGTGTGCCCTGATACCCCTGAGGGTGGCTGC CTCCTCTGGTCCCCAGGCCCCTCCAGTTCATCGGAATTCAGCCATGAAACTGACTCATCAGTTGAGGGC AGCTCCAGTGCCAGCAATAGGGTGGCACAGAAGCTCATCTCAGTCATCCTGCAGGCACATGAGGGGGTG CCCTCCGGCCACCTGTACTGTATCAGCCCCAAGCAGGTGGTGGTCCCTGCTGGGGGCAGCAGTACCATC TACATCTCCTTCACCCCTATGGTGCTCAGCCCTGAGATCCTGCACAAGGTGGAGTGTACTGGCTACGCC CTGGGTTTCATGAGCTTGGACAGCAAGGTGGAAAGGGAGATTCCAGGGAAGAGGCATCGCCTGCAGGAC TTTGCGGTGGGACCCCTGAAACTGGACCTGCATAGCTACGTGAGGCCTGCACAGCTAAGTGTGGAGCTG GACTACGGCGGCAGTATGGAATTCCAGTGCCAGGCCAGTGACCTCATTCCCGAGCAGCCCTGCTCTGGG GTGCTGAGTGAGCTGGTGACCACCCACCACCTGAAGCTGACCAACACTACAGAGATCCCACACTACTTC CGGCTTATGGTCTCCAGGCCCTTCTCCGTTTCTCAAGATGGGGCGAGCCAGGACCACAGAGCTCCTGGC CCTGGCCAGAAGCAGGAGTGTGAGGAGGAGACAGCCTCAGCGGACAAGCAGCTGGTGCTCCAAGCACAG GAGAACATGCTGGTGAACGTGTCCTTCTCACTCTCCCTGGAGCTGCTCTCCTATCAGAAGCTCCCAGCT GACCAGACACTGCCTGGGGTGGACATTCAGCAGAGTGCGAGTGGAGAGAGAGAGATGGTGTTTACTCAG AACCTGCTCCTGGAGTACACCAACCAGACCACTCAGGTGGTGCCCCTGCGGGCTGTGGTGGCCGTGCCT GAGCTGCAGCTCTCCACCAGCTGGGTGGACTTTGGGACCTGCTTTGTGAGCCAGCAGCGAGTCCGGGAG GTCTACCTGATGAACCTGAGCGGGTGCCGAAGCTACTGGACTATGCTGATGGGAGTAGTGAGCTGTACG AGTCCACGATGGTGGTGGAAGGTGTGCTCGGTGAGAAGTCCTGCACCCTGCGGCTCCGGGGCCAAGGCT CCTATGATGAGAGATACATGTTGCCTCACCAGCCCTGAGGCTCCGCCCCAGCCCTCAGCCCCAGGCCCC AGCTGGAGAAAAAACATTGCCCAGGGATTAGGAGCAGCTCTTCAGCACAAAGACACAGACTTGGGGACC TGGGGACCTCTGGGCAGCTCCTGGAATGGAAGAACCCCCTTCCACAATGGTCTCAGCCTAGGCCCTCAT GATATGTCCTCAGAGCTAACATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_007337
Insert Size	5337 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007337.3
RefSeq Size	5511 bp
RefSeq ORF	5337 bp
Locus ID	9940
UniProt ID	Q9Y238
MW	197.9 kDa
Gene Summary	The cytogenetic location of this gene is 3p21.3, and it is located in a region that is commonly deleted in a variety of malignancies. Down-regulation of this gene has been observed in several human cancers including lung, esophageal, renal tumors, and head and neck squamous cell carcinoma. In some cases, reduced expression of this gene in tumor cells is a result of aberrant promoter methylation. Several alternatively spliced transcripts have been observed that contain disrupted coding regions and likely encode nonfunctional proteins.[provided by RefSeq, Mar 2016] Transcript Variant: This variant (DLEC1-S3) lacks an exon in the 3' coding region that results in a frameshift and use of a downstream stop codon, compared to variant DLEC1-N1. The encoded isoform (DLEC1-S3) has a longer, distinct C-terminus compared to the DLEC1-N1 isoform. There are currently no publicly available full-length transcripts supporting this variant, but it is supported by data in PMID:10213508.

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