PLCH2 (NM_014638) Human Untagged Clone
CAT＃: SC304146
PLCH2 (untagged)-Human phospholipase C, eta 2 (PLCH2)
CNY 27270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PLC-eta2; PLC-L4; PLCeta2; PLCL4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC304146 representing NM_014638.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCTGGTCCATGGCCCTCCCCCGACAGCCGGACCAAGGGAACGGTGGCCTGGCTGGCGGAGGTACTC CTCTGGGTTGGAGGGAGTGTGGTGCTGTCTTCAGAGTGGCAGCTCGGCCCCCTGGTGGAGCGGTGCATG GGTGCCATGCAAGAGGGGATGCAGATGGTGAAGCTGCGTGGCGGCTCCAAGGGCCTGGTCCGCTTCTAC TACCTGGACGAGCACCGCTCCTGCATCCGCTGGAGGCCCTCACGCAAGAACGAGAAGGCCAAGATCTCC ATCGACTCCATCCAGGAGGTGAGTGAGGGGCGGCAGTCGGAGGTCTTCCAGCGCTACCCTGACGGCAGC TTCGACCCCAACTGCTGCTTCAGCATCTACCACGGCAGCCACCGCGAGTCGCTGGACCTGGTCTCCACC AGCAGCGAGGTGGCGCGCACCTGGGTCACTGGCCTGCGCTACCTCATGGCCGGCATCAGCGACGAGGAC AGCCTGGCTCGCCGCCAGCGCACCAGGGACCAGTGGCTGAAGCAGACGTTTGACGAGGCCGACAAGAAC GGGGATGGCAGCCTGAGCATTGGCGAGGTCCTGCAGCTGCTGCACAAGCTCAACGTGAACCTGCCCCGG CAGAGGGTGAAGCAGATGTTCAGGGAAGCGGACACGGATGACCACCAAGGGACGCTGGGTTTTGAAGAG TTCTGTGCCTTCTACAAGATGATGTCCACCCGCCGGGACCTCTACCTGCTCATGCTGACCTACAGCAAC CACAAGGACCACCTGGATGCCGCCAGCCTGCAGCGCTTCCTGCAGGTGGAGCAGAAGATGGCGGGTGTG ACCCTCGAGAGCTGCCAGGACATCATCGAGCAGTTTGAGCCATGCCCAGAAAACAAGAGTAAGGGGCTG CTGGGCATTGATGGCTTCACCAACTACACCAGGAGCCCTGCTGGTGACATCTTCAACCCTGAGCACCAC CATGTGCACCAGGACATGACGCAGCCGCTGAGCCACTACTTCATCACCTCGTCCCACAACACCTACCTC GTGGGTGACCAGCTCATGTCCCAGTCACGGGTGGACATGTATGCTTGGGTCCTGCAGGCTGGCTGCCGC TGCGTGGAGGTGGACTGCTGGGATGGGCCCGACGGGGAGCCCATTGTGCACCATGGCTACACTCTGACT TCCAAGATCCTCTTCAAAGACGTCATTGAAACCATCAACAAATATGCCTTCATCAAGAATGAGTACCCA GTGATCCTGTCCATCGAAAACCACTGCAGTGTCATCCAGCAGAAGAAAATGGCCCAGTATCTGACTGAC ATCCTTGGGGACAAGCTGGACCTGTCATCAGTGAGCAGTGAAGATGCCACCACACTCCCCTCTCCACAG ATGCTCAAGGGCAAGATCCTCGTGAAGGGGAAGAAGCTCCCAGCCAACATCAGCGAGGATGCGGAGGAA GGCGAGGTGTCTGATGAGGACAGTGCTGATGAGATTGACGATGACTGCAAGCTCCTCAATGGGGATGCA TCCACCAATCGAAAGCGTGTAGAAAACACTGCTAAGAGGAAACTGGATTCCCTCATCAAAGAGTCGAAG ATTCGGGACTGTGAGGACCCCAACAACTTCTCCGTCTCCACACTGTCCCCATCTGGAAAGCTCGGACGC AAGAGCAAGGCTGAAGAGGACGTGGAGTCTGGGGAGGATGCCGGGGCCAGCAGACGCAATGGCCGCCTC GTCGTGGGAAGCTTCTCCAGGCGCAAGAAGAAGGGCAGCAAGCTGAAGAAGGCGGCCAGCGTGGAGGAG GGAGATGAGGGTCAGGACTCCCCGGGAGGCCAGAGCCGAGGGGCGACCCGGCAGAAGAAGACCATGAAG CTGTCCCGGGCCCTCTCTGACCTGGTGAAGTACACCAAGTCCGTGGCCACCCACGACATAGAGATGGAG GCGGCGTCCAGCTGGCAGGTGTCGTCCTTCAGCGAGACCAAGGCCCACCAGATTCTGCAGCAGAAGCCG GCGCAGTACCTACGCTTCAACCAGCAGCAGCTCTCCCGCATCTACCCCTCCTCCTACCGTGTGGACTCC AGCAACTACAACCCGCAGCCCTTCTGGAACGCCGGCTGCCAAATGGTTGCCCTGAACTACCAGTCAGAG GGGCGGATGCTGCAGCTGAACCGAGCCAAGTTCAGCGCCAACGGTGGCTGCGGCTACGTACTCAAGCCT GGGTGCATGTGCCAGGGCGTGTTCAACCCCAACTCGGAGGACCCCCTGCCCGGGCAGCTCAAGAAGCAG CTGGTGCTCCGGATCATCAGTGGCCAGCAGCTTCCCAAGCCGCGCGACTCCATGCTGGGGGACCGTGGG GAGATCATCGACCCCTTTGTGGAGGTGGAGATCATTGGGCTCCCTGTGGACTGCAGCAGGGAGCAGACC CGCGTGGTGGACGACAACGGGTTCAACCCCACCTGGGAGGAGACCCTGGTTTTCATGGTGCACATGCCG GAGATCGCGCTGGTCCGCTTCCTCGTCTGGGACCACGATCCCATCGGGCGTGACTTCATTGGCCAGAGG ACGCTGGCCTTCAGCAGCATGATGCCAGGCTACAGACACGTGTACCTAGAAGGGATGGAAGAGGCCTCC ATCTTCGTGCATGTGGCTGTCAGTGACATCAGCGGTAAGGTCAAGCAGGCTCTGGGCCTAAAAGGCCTC TTCCTCCGAGGCCCAAAGCCCGGCTCGCTGGACAGTCATGCTGCTGGGCGGCCCCCGGCCCGGCCCTCC GTTAGCCAGCGGATCCTGCGGCGCACGGCCAGCGCCCCGACCAAGAGCCAGAAGCCGGGCCGCAGGGGC TTCCCGGAGCTGGTCCTGGGTACACGGGACACAGGCTCCAAGGGGGTGGCAGACGATGTGGTGCCCCCC GGGCCCGGACCTGCTCCGGAAGCCCCAGCCCAGGAGGGGCCCGGCAGCGGCAGCCCCCGAGACACCCGC CCCCTCTCCACGCAGCGGCCACTCCCCCCACTGTGCAGCCTGGAAACCATCGCTGAGGAGCCCGCCCCA GGCCCTGGTCCCCCGCCACCAGCGGCTGTCCCCACCAGCTCTTCTCAGGGACGGCCCCCATACCCCACA GGACCCGGAGCCAATGTGGCAAGCCCCCTAGAGGACACTGAGGAGCCCCGAGACAGCAGGCCTCGGCCG TGCAACGGCGAGGGCGCCGGCGGGGCATACGAGAGGGCCCCCGGCAGCCAGACGGACGGCAGGAGCCAG CCCCGGACCCTGGGCCACCTGCCCGTGATTAGAAGGGTGAAGAGTGAGGGGCAGGTGCCCACGGAGCCC CTGGGAGGGTGGCGGCCCCTGGCCGCTCCCTTTCCAGCTCCTGCCGTGTACTCCGATGCCACGGGCAGT GACCCGCTGTGGCAGCGGCTGGAGCCATGTGGCCACCGAGACAGCGTTTCCTCCTCCTCCAGCATGTCA TCCAGCGACACTGTCATTGACCTCTCCCTGCCCAGCCTGGGCCTGGGCCGCAGCCGTGAGAACCTCGCT GGAGCCCACATGGGACGCCTGCCCCCCAGGCCCCACTCGGCTTCGGCTGCCCGCCCAGACCTGCCACCT GTGACCAAGAGCAAATCCAACCCCAACCTTCGGGCTACAGGCCAGCGGCCTCCCATACCTGACGAACTG CAGCCCAGGTCCCTGGCCCCAAGGATGGCTGGCCTCCCCTTCCGGCCTCCCTGGGGCTGCCTTTCCCTG GTGGGCGTGCAGGACTGCCCCGTGGCTGCCAAGTCCAAGAGCCTGGGCGACCTCACTGCTGATGACTTT GCCCCTAGCTTTGAGGGCGGCTCCCGCAGACTGAGCCACAGCCTGGGCCTCCCGGGAGGGACACGGCGG GTGTCGGGGCCAGGGGTGAGACGGGACACCCTGACAGAGCAGCTGCGCTGGCTCACTGTCTTCCAGCAG GCAGGAGACATCACGTCACCCACCAGCCTGGGCCCGGCTGGGGAGGGGGTGGCAGGGGGCCCTGGTTTT GTGCGGCGCTCCTCCTCCCGCAGCCACAGCCGCGTGCGTGCCATTGCCAGCCGGGCCCGCCAGGCCCAG GAGCGGCAGCAGAGACTGCAGGGCCTGGGCCGGCAGGGACCCCCAGAAGAGGAGCGGGGCACCCCCGAG GGCGCCTGCTCCGTGGGCCACGAGGGCAGTGTGGATGCACCAGCACCCTCCAAGGGAGCCCTCGGGCCA GCATCCGCGGCTGCTGAAAACCTGGTCCTGCTCCGCCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_014638 |
| Insert Size | 4251 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014638.3 |
| RefSeq Size | 4857 bp |
| RefSeq ORF | 4251 bp |
| Locus ID | 9651 |
| UniProt ID | O75038 |
| Protein Families | Druggable Genome |
| MW | 154.7 kDa |
| Gene Summary | PLCH2 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave PtdIns(4,5) P2 to generate second messengers inositol 1,4,5-trisphosphate and diacylglycerol (Zhou et al., 2005 [PubMed 16107206]).[supplied by OMIM, Jun 2009] Transcript Variant: This variant (1) encodes the longest isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211882 | PLCH2 (Myc-DDK-tagged)-Human phospholipase C, eta 2 (PLCH2) |
CNY 9688.00 |
|
| RC211882L3 | Lenti-ORF clone of PLCH2 (Myc-DDK-tagged)-Human phospholipase C, eta 2 (PLCH2) |
CNY 16060.00 |
|
| RC211882L4 | Lenti-ORF clone of PLCH2 (mGFP-tagged)-Human phospholipase C, eta 2 (PLCH2) |
CNY 16060.00 |
|
| RG211882 | PLCH2 (tGFP-tagged) - Human phospholipase C, eta 2 (PLCH2) |
CNY 15580.00 |
