PDCD11 (NM_014976) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ALG-4; ALG4; NFBP; RRP5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC304191 representing NM_014976. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAAACCTGGAAGAAAGCTTCCCCCGAGGAGGTACAAGAAAGATCCACAAACCAGAGAAAGCTTTC CAGCAGTCAGTTGAACAAGACAACTTATTTGATATTTCTACTGAAGAGGGATCCACCAAAAGAAAAAAG AGCCAGAAGGGGCCAGCAAAAACAAAAAAGTTGAAAATCGAAAAGAGAGAAAGCAGCAAGTCCGCAAGA GAGAAGTTTGAAATCCTTAGTGTTGAGTCCCTGTGTGAGGGAATGCGTATTTTGGGTTGCGTGAAAGAG GTGAATGAACTGGAACTGGTGATTAGTCTCCCCAATGGCCTCCAGGGCTTTGTGCAAGTCACTGAAATC TGTGATGCCTACACCAAAAAGCTGAATGAGCAGGTGACACAAGAACAACCTCTGAAGGACCTACTTCAC TTGCCTGAACTTTTCTCACCTGGAATGCTGGTAAGATGTGTGGTGAGCAGTCTGGGCATCACAGACAGG GGCAAGAAGAGTGTCAAGCTGTCTCTGAACCCCAAAAATGTCAACAGAGTGCTGAGTGCTGAGGCCCTG AAGCCTGGCATGCTACTTACAGGTACCGTATCCAGCCTGGAAGACCATGGCTACCTAGTGGACATTGGT GTTGATGGGACCAGAGCTTTTCTGCCACTGCTGAAAGCCCAGGAGTACATCAGACAGAAGAACAAAGGT GCTAAACTAAAGGTGGGTCAGTACCTGAACTGCATTGTTGAAAAGGTGAAAGGCAACGGAGGAGTTGTT AGTCTGTCTGTTGGTCACTCAGAGGTTTCTACGGCCATTGCTACTGAACAGCAGAGCTGGAACCTTAAT AACTTGCTACCAGGACTGGTGGTCAAAGCTCAGGTACAGAAGGTGACTCCATTTGGCCTTACGCTAAAC TTCCTCACATTCTTCACGGGCGTGGTTGACTTTATGCACCTGGATCCCAAGAAAGCTGGAACATATTTC TCAAATCAGGCAGTGAGGGCCTGCATCCTTTGCGTCCATCCTCGAACCAGAGTTGTGCACCTGAGCCTG CGCCCCATCTTCCTACAGCCTGGACGCCCACTCACCCGACTCTCTTGCCAGAACCTTGGAGCAGTGCTG GATGATGTTCCTGTCCAGGGTTTTTTCAAAAAGGCTGGGGCCACCTTTAGGCTGAAGGATGGGGTTCTG GCCTATGCCCGGCTCAGCCATCTCTCTGATTCTAAGAACGTCTTCAATCCTGAGGCCTTCAAGCCAGGG AACACTCACAAGTGTAGAATTATTGACTACAGCCAAATGGATGAACTGGCCTTGCTCTCTCTACGAACG TCTATTATTGAAGCTCAGTACCTTAGATATCATGACATCGAACCTGGGGCAGTGGTAAAGGGCACAGTG CTAACCATAAAGTCATATGGGATGCTGGTGAAGGTGGGCGAGCAGATGAGGGGCCTGGTACCTCCCATG CACCTGGCTGACATCCTGATGAAGAATCCGGAGAAGAAGTACCACATCGGGGATGAGGTCAAGTGCCGG GTTTTGCTTTGTGACCCTGAAGCCAAGAAGCTGATGATGACCCTGAAAAAAACCCTGATTGAGTCCAAA CTACCTGTCATTACCTGCTATGCCGATGCCAAGCCTGGTCTGCAGACACATGGCTTCATCATCAGGGTC AAGGACTATGGCTGCATTGTGAAGTTCTACAACAATGTGCAGGGACTGGTGCCCAAGCATGAGCTCAGT ACTGAGTATATCCCTGACCCGGAGAGAGTTTTTTACACTGGCCAGGTGGTGAAGGTTGTCGTATTGAAC TGTGAGCCATCCAAAGAGAGGATGCTCTTATCCTTCAAGCTGTCGAGTGATCCAGAGCCAAAGAAAGAG CCTGCAGGACACAGTCAGAAGAAAGGAAAAGCCATTAACATTGGGCAGTTGGTAGATGTGAAGGTTTTA GAGAAGACCAAAGATGGGCTGGAGGTGGCTGTCCTGCCCCACAACATCCGTGCTTTCCTCCCCACATCT CATCTGTCGGACCACGTTGCCAACGGCCCATTGTTACATCATTGGCTCCAGGCAGGTGACATCCTTCAC CGAGTCCTGTGTCTGAGCCAGAGCGAGGGGCGTGTTCTTCTTTGCAGGAAGCCAGCCTTGGTCTCCACA GTAGAAGGTGGCCAGGATCCCAAGAACTTCTCAGAAATCCATCCTGGAATGCTGCTCATTGGTTTTGTG AAGAGCATCAAGGACTATGGCGTGTTCATCCAGTTCCCCTCAGGTCTTAGCGGACTGGCCCCAAAAGCT ATCATGAGTGACAAATTTGTGACCTCCACAAGTGACCACTTTGTTGAGGGCCAGACAGTAGCGGCAAAG GTGACCAATGTGGATGAGGAGAAGCAGCGGATGCTGCTGTCACTGCGGCTGTCGGACTGTGGTCTGGGG GACTTGGCTATCACCAGCCTCCTCCTCCTGAATCAGTGCCTGGAGGAGCTGCAGGGCGTGCGCAGCCTT ATGAGCAACCGAGACTCTGTGTTGATCCAGACGCTGGCCGAGATGACCCCAGGAATGTTCCTTGACCTA GTGGTGCAGGAGGTGTTGGAAGATGGCTCTGTGGTATTCAGTGGGGGTCCAGTGCCCGACCTGGTCCTG AAAGCCAGCAGATACCATCGCGCAGGGCAGGAGGTGGAATCTGGGCAGAAAAAGAAGGTTGTTATCTTA AATGTTGATCTTTTGAAGTTGGAAGTGCACGTTTCCCTTCACCAGGACTTGGTGAATAGAAAAGCTAGA AAGCTGAGGAAAGGCAGCGAACACCAGGCGATTGTGCAGCACTTGGAGAAGTCCTTTGCCATTGCCTCC TTGGTAGAGACGGGCCACCTGGCAGCTTTCTCCCTGACCTCTCACCTCAACGACACCTTCCGCTTTGAC TCAGAGAAATTGCAGGTGGGACAGGGTGTCTCCCTAACCCTCAAGACCACAGAACCAGGAGTGACTGGC CTTCTTTTGGCTGTGGAGGGGCCGGCTGCCAAGAGGACCATGAGGCCGACCCAGAAGGACTCTGAGACA GTTGATGAGGATGAAGAAGTGGATCCAGCTCTGACTGTAGGGACCATAAAGAAGCACACCCTCTCCATC GGGGACATGGTCACAGGGACTGTCAAGTCCATTAAGCCTACCCATGTGGTTGTGACTCTGGAAGATGGC ATTATTGGCTGTATCCATGCCTCCCACATTCTAGATGATGTTCCAGAGGGCACCTCTCCTACTACCAAG CTGAAGGTTGGGAAGACGGTCACTGCCCGAGTGATTGGCGGGCGAGACATGAAGACATTCAAGTATCTC CCAATAAGTCACCCCAGATTCGTTCGAACCATCCCGGAGCTGAGTGTTCGGCCAAGTGAGCTGGAGGAT GGCCACACTGCTCTTAACACTCACTCTGTTAGCCCCATGGAGAAGATTAAACAGTACCAGGCCGGCCAG ACTGTTACTTGCTTCTTAAAGAAATACAATGTGGTGAAGAAATGGCTTGAGGTGGAGATTGCCCCAGAC ATCCGGGGGAGAATTCCCTTATTGCTCACTTCTCTGAGCTTCAAGGTTCTGAAGCATCCAGATAAGAAG TTCCGGGTTGGCCAGGCCCTGAGGGCCACCGTTGTTGGCCCAGATTCCTCCAAGACCCTCTTATGTCTG TCCCTCACAGGTCCTCACAAGCTTGAGGAAGGGGAAGTGGCCATGGGCCGAGTGGTGAAGGTGACTCCC AACGAGGGGCTGACCGTCTCCTTCCCCTTTGGGAAGATAGGAACAGTCAGTATATTTCACATGAGTGAC TCCTACTCCGAGACGCCCCTGGAAGACTTCGTCCCCCAGAAGGTTGTCAGATGTTACATCCTGTCCACT GCAGACAACGTATTGACTTTGTCGCTGCGATCATCCAGAACAAACCCGGAGACGAAAAGCAAAGTAGAA GATCCAGAGATTAACTCCATCCAGGACATTAAGGAAGGGCAGCTTCTGAGGGGCTATGTAGGGTCCATC CAGCCACACGGTGTGTTCTTTCGCCTTGGCCCCTCCGTTGTGGGTTTGGCTCGGTACTCCCATGTCTCC CAGCACAGCCCGTCCAAGAAAGCCCTTTATAACAAACACCTCCCTGAAGGGAAGCTGCTCACAGCCAGG GTCCTACGCCTTAACCACCAGAAGAACCTGGTAGAGCTGTCTTTCCTCCCCGGAGACACTGGGAAGCCA GACGTGCTTTCTGCTTCCTTGGAAGGGCAACTTACAAAGCAAGAGGAGAGGAAAACAGAGGCTGAGGAG AGAGACCAAAAAGGGGAAAAGAAAAATCAGAAAAGGAACGAGAAGAAGAACCAGAAGGGGCAGGAGGAG GTGGAGATGCCCAGCAAGGAGAAGCAACAGCCCCAGAAGCCACAGGCGCAGAAGCGGGGCGGGCGGGAG TGCCGGGAGTCTGGGAGTGAGCAGGAAAGAGTGAGCAAGAAGCCAAAGAAAGCCGGCCTGTCAGAGGAG GACGACAGCCTTGTGGACGTGTACTATCGGGAGGGAAAAGAGGAGGCAGAAGAGACGAATGTGCTGCCC AAGGAGAAGCAAACCAAGCCAGCAGAAGCGCCCCGGCTGCAGCTGTCTTCAGGCTTCGCTTGGAATGTG GGACTAGACTCTCTGACCCCGGCCTTGCCACCTCTAGCAGAGAGCTCAGACAGCGAGGAGGATGAGAAG CCACACCAAGCCACGATAAAGAAAAGCAAGAAAGAAAGGGAGTTGGAGAAGCAGAAGGCAGAGAAGGAA CTGTCCCGCATTGAGGAGGCGCTGATGGATCCTGGGCGGCAGCCAGAGTCCGCGGATGATTTTGACCGA CTGGTGCTGAGCTCCCCCAACAGCTCCATTCTGTGGCTGCAGTACATGGCTTTCCACCTGCAGGCCACG GAGATCGAGAAGGCCCGTGCCGTGGCTGAGAGGGCCCTTAAGACCATCTCCTTCAGAGAGGAGCAGGAG AAGCTGAACGTGTGGGTGGCTCTGCTGAACCTGGAGAACATGTACGGCTCTCAGGAGTCCCTGACCAAG GTCTTTGAGCGAGCCGTGCAGTACAACGAGCCTCTCAAAGTCTTTCTCCACCTGGCTGACATCTACGCC AAGTCAGAGAAATTCCAGGAAGCTGGTGAACTCTACAACCGGATGCTGAAGCGTTTCCGGCAGGAGAAA GCTGTGTGGATCAAATACGGCGCCTTCCTTCTGCGGAGGAGCCAGGCTGCAGCCAGTCACCGCGTGCTG CAGCGAGCCCTGGAGTGCCTGCCTAGCAAGGAGCATGTGGATGTCATTGCCAAGTTTGCCCAGCTTGAG TTTCAGCTGGGGGATGCAGAGCGGGCCAAAGCCATTTTTGAGAACACGCTGAGCACCTACCCAAAGCGC ACAGATGTCTGGTCGGTCTATATCGACATGACCATCAAGCACGGCAGCCAGAAGGACGTCCGGGACATC TTTGAGCGGGTCATTCATCTGAGCTTGGCCCCCAAGAGAATGAAGTTCTTCTTCAAGCGCTACCTGGAC TACGAGAAGCAGCATGGCACTGAGAAGGATGTGCAGGCAGTCAAGGCCAAGGCCCTGGAGTATGTGGAG GCCAAGAGCTCAGTGCTAGAGGACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_014976
Insert Size	5616 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_014976.1
RefSeq Size	6416 bp
RefSeq ORF	5616 bp
Locus ID	22984
UniProt ID	Q14690
MW	208.7 kDa
Gene Summary	PDCD11 is a NF-kappa-B (NFKB1; 164011)-binding protein that colocalizes with U3 RNA (MIM 180710) in the nucleolus and is required for rRNA maturation and generation of 18S rRNA (Sweet et al., 2003 [PubMed 14624448]; Sweet et al., 2008 [PubMed 17654514]).[supplied by OMIM, Oct 2008]

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