SIPA1L3 (NM_015073) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CTRCT45; SPAL3; SPAR3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC304219 representing NM_015073. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCACCTATCGGGCCATCCCCAGCGATGGTGTGGACCTGGCAGCCAGCTGTGGCGCCAGGGTGGGC GATGTCCTCCCTGGGCCACACACAGGGGACTACGCTCCCTTGGGATTCTGGGCCCAGAATGGCAGCATG TCCCAGCCTCTTGGCGAGAGCCCGGCCACCGCCACCGCCACCGCCACCGCCACCACCCGCCCCAGCCCC ACCACTCCCGCAATGCCCAAGATGGGCGTGCGCGCAAGGGTGGCCGACTGGCCGCCCAAGCGGGAGGCC CTGAGAGAGCACAGCAACCCAAGCCCCTCCCAGGACACAGATGGCACAAAGGCCACCAAGATGGCCCAT TCCATGAGGAGCATACAGAACGGACAGCCCCCCACCAGCACCCCGGCTTCCTCAGGGTCCAAAGCCTTC CACCGACTCTCCAGGAGAAGGTCCAAAGACGTGGAGTTCCAGGACGGGTGGCCCCGGTCCCCCGGCAGG GCCTTCCTCCCCCTTCGGCACCGCAGCAGCAGCGAGATCACCCTCAGCGAGTGTGACGCGGAGGACGCG GGGGAGCCGCGGGGGGCCCGGCACACGGGGGCGCTGCCCCTCTTCCGCGAGTACGGGAGCACCTCGTCC ATCGACGTGCAGGGCATGCCCGAGCAGAGCTTCTTCGACATCCTGAACGAGTTCCGCAGCGAGCAGCCC GACGCCCGAGGGTGCCAGGCCCTCACCGAGCTCCTCCGGGCAGATCCTGGCCCACACCTCATGGGGGGC GGCGGCGGAGCCAAGGGGGACTCCCACAACGGGCAGCCCGCCAAGGACAGCCTCCTGCCACTGCAGCCC ACGAAGGAGAAGGAGAAGGCCCGGAAGAAACCTGCGCGGGGCCTCGGCGGCGGGGACACGGTGGACTCG TCCATCTTTCGGAAGCTAAGGAGCAGCAAACCCGAGGGGGAGGCTGGGCGTTCCCCGGGGGAGGCCGAC GAGGGCCGGAGCCCCCCGGAAGCCAGCAGGCCGTGGGTGTGTCAGAAGAGCTTCGCCCACTTCGACGTG CAGAGCATGCTGTTCGACCTCAACGAGGCGGCCGCCAACAGGGTGTCGGTGTCGCAGCGGCGGAACACC ACCACGGGTGCTTCGGCCGCTTCCGCCGCCTCGGCCATGGCCTCCCTCACGGCCTCGCGGGCCCACAGC CTCGGAGGCCTGGACCCGGCCTTCACCAGCACAGAGGACCTAAACTGCAAGGAGAACTTGGAGCAGGAC CTCGGCGATGACAACAGCAACGACCTGCTGCTCAGCTGCCCGCACTTCCGCAATGAGATCGGGGGCGAG TGTGAGCGCAACGTGAGCTTCTCCCGGGCTTCCGTGGGCTCCCCGAGCAGCGGCGAGGGCCACCTGGCA GAGCCCGCCCTGAGCGCCTACCGCACCAACGCCAGCATCTCGGTGTTGGAAGTTCCCAAGGAGCAGCAG CGGACGCAGAGTCGGCCCCGGCAGTACAGCATCGAGCATGTGGACCTGGGCGCCCGCTACTACCAGGAT TACTTCGTGGGCAAAGAACATGCCAATTACTTCGGCGTGGATGAGAAGCTGGGGCCAGTGGCTGTGAGC ATTAAGCGGGAGAAGCTGGAAGACCACAAGGAGCACGGACCTCAGTACCAGTACAGGATCATCTTCCGG ACCCGCGAGCTCATCACCCTGCGGGGCTCCATCCTGGAAGATGCTACGCCCACAGCCACCAAGCATGGG ACCGGGCGGGGCCTGCCCTTGAAGGATGCCCTGGAGTATGTCATCCCCGAGCTCAACATCCACTGCCTG CGGCTGGCCCTCAACACCCCCAAGGTGACGGAGCAACTGCTGAAGCTCGATGAGCAAGGGCTCTGCCGG AAGCACAAGGTGGGCATCCTCTATTGCAAGGCCGGCCAGAGCTCCGAGGAGGAGATGTACAACAATGAG GAGGCCGGCCCCGCCTTTGAGGAGTTCCTCTCCCTCATCGGCGAGAAGGTCTGCCTGAAGGGCTTCACC AAGTACGCTGCCCAGCTGGACGTCAAGACCGACTCCACGGGAACCCACTCCCTCTACACGATGTACCAG GACTACGAGATCATGTTCCATGTCTCCACCCTGCTCCCTTACACCCCCAACAACAGGCAGCAGCTGCTA CGGAAGAGGCACATAGGAAATGACATCGTGACGATCATCTTCCAGGAGCCTGGCGCGCTACCGTTCACC CCCAAGAACATCCGCTCCCACTTCCAGCACGTCTTCATCATTGTCCGAGTCCACAACCCCTGCACTGAT AACGTCTGTTACAGTATGGCTGTGACCCGATCCAAAGACGCTCCTCCTTTCGGCCCCCCCATCCCCAGT GGAACCACATTCCGCAAATCCGACGTCTTCAGAGACTTCTTGCTGGCCAAGGTGATTAACGCTGAGAAC GCCGCGCACAAGTCCGACAAGTTCCACACCATGGCCACCAGGACCCGCCAGGAGTATCTCAAGGACCTG GCCGAAAACTGTGTCTCCAACACCCCCATCGACTCCACCGGCAAATTCAACCTCATCTCCCTGACCTCC AAGAAGAAGGAAAAGACAAAAGCACGGGCTGGCGCTGAGCAGCACAGTGCAGGGGCCATCGCCTGGAGG GTGGTGGCCCAGGACTACGCCCAGGGGGTGGAAATCGACTGCATTTTGGGAATTTCCAATGAGTTTGTG GTGCTCCTGGACTTACGCACCAAGGAGGTGGTGTTCAACTGCTACTGCGGGGATGTCATTGGCTGGACT CCAGACTCCTCCACACTCAAAATCTTCTATGGACGAGGAGACCACATCTTCCTACAGGCGACAGAGGGT TCTGTGGAGGACATAAGGGAGATAGTGCAGAGACTGAAGGTGATGACCAGTGGCTGGGAGACGGTGGAC ATGACGCTTCGGCGGAACGGGCTCGGGCAGCTGGGCTTCCACGTGAAGTACGACGGCACGGTGGCCGAG GTTGAGGACTATGGGTTCGCCTGGCAGGCCGGCCTCCGGCAGGGCAGCCGACTAGTGGAGATCTGCAAG GTGGCCGTGGTCACACTGACCCACGACCAGATGATCGACCTGCTGCGCACCTCTGTCACTGTGAAGGTG GTCATCATCCCGCCTTTTGAGGACGGCACTCCCCGGAGGGGTTGGCCGGAGACCTACGACATGAATACC TCGGAGCCCAAGACGGAGCAGGAAAGCATCACTCCTGGGGGCCGGCCCCCCTACCGCAGCAATGCTCCC TGGCAGTGGAGCGGGCCCGCATCCCATAACTCTCTACCAGCCTCCAAGTGGGCCACTCCAACCACTCCC GGCCATGCCCAGTCCCTGAGCCGGCCCCTGAAGCAGACCCCCATAGTCCCCTTCCGGGAGTCCCAGCCA CTGCACAGCAAGAGGCCTGTCAGCTTCCCAGAAACCCCTTACACAGTATCACCAGCAGGGGCCGACAGA GTCCCTCCCTACCGACAGCCTTCTGGGAGCTTCTCCACCCCCGGTTCGGCCACCTACGTGAGATACAAG CCATCCCCAGAAAGGTACACGGCTGCCCCACACCCCCTGCTATCTCTTGATCCCCACTTCAGCCACGAT GGGACGTCCAGCGGCGACTCCTCTTCCGGCGGCCTGACCAGCCAGGAGAGCACCATGGAACGCCAGAAG CCAGAGCCTTTGTGGCATGTGCCTGCCCAGGCCAGGCTCTCAGCCATAGCCGGAAGCAGCGGGAACAAG CACCCGTCCAGGCAGGATGCAGCAGGCAAAGATTCCCCCAACAGGCATTCCAAAGGAGAACCTCAATAC TCAAGTCATTCCAGCAGCAACACCCTCTCCAGCAACGCATCCAGCAGCCACAGCGACGACCGCTGGTTC GACCCCCTGGACCCCCTGGAGCCAGAGCAAGACCCCCTCTCCAAGGGTGGCTCTAGTGACAGCGGCATC GACACCACCCTCTACACCTCCAGCCCTAGCTGCATGTCCCTGGCCAAGGCTCCACGGCCCGCCAAGCCA CACAAGCCCCCTGGAAGTATGGGCCTTTGTGGCGGGGGTCGCGAGGCCGCTGGGAGGTCCCACCACGCA GACAGGCGGCGGGAGGTCTCCCCTGCCCCCGCAGTTGCCGGCCAAAGCAAGGGCTACCGACCGAAGCTG TACTCCTCCGGCTCCAGCACCCCCACGGGACTGGCGGGGGGCAGCCGAGACCCACCGAGGCAGCCCAGT GACATGGGCTCGAGGGTTGGCTACCCCGCTCAGGTTTACAAAACTGCCAGTGCAGAGACTCCTCGGCCC TCCCAGCTGGCCCAGCCCAGCCCCTTTCAGCTCTCCGCCTCCGTCCCCAAGTCCTTCTTCTCCAAGCAG CCTGTACGCAATAAGCACCCAACAGGGTGGAAGAGAACGGAGGAGCCCCCACCACGGCCACTCCCCTTC AGTGACCCAAAGAAGCAGGTGGACACGAACACCAAAAATGTCTTTGGGCAACCGAGGTTGAGGGCATCC CTCCGAGACCTCCGGTCACCACGGAAGAACTACAAATCCACCATCGAGGATGACCTGAAGAAACTCATC ATCATGGACAACCTGGGGCCAGAGCAGGAGAGAGACACGGGACAGTCACCGCAGAAGGGCCTGCAGCGG ACGCTGTCGGACGAGAGCCTGTGCAGCGGGCGCCGGGAGCCCAGCTTCGCCAGCCCCGCTGGCCTAGAG CCAGGGCTGCCCAGCGACGTGCTCTTCACCAGCACCTGCGCCTTCCCGTCCAGCACGCTGCCTGCACGC CGCCAGCACCAGCACCCCCACCCGCCCGTCGGCCCCGGTGCCACCCCTGCCGCCGGCAGCGGCTTTCCC GAGAAGAAATCCACCATCTCAGCCTCGGAGCTCTCGCTGGCTGATGGGCGGGACCGCCCCCTGCGGCGC CTGGACCCTGGGCTGATGCCCCTGCCTGACACAGCTGCTGGCCTCGAGTGGTCCAGCCTGGTGAACGCA GCCAAGGCATACGAAGTGCAAAGAGCCGTCTCACTCTTCTCTCTGAACGACCCGGCCCTGAGCCCGGAC ATCCCGCCTGCACACAGTCCTGTCCACAGCCACCTGAGCCTGGAGAGGGGACCCCCGACCCCCAGGACC ACCCCTACCATGAGCGAGGAGCCACCCCTGGATCTGACAGGCAAGGTGTACCAGCTGGAGGTGATGCTG AAACAGCTGCACACTGACCTGCAGAAGGAGAAGCAGGACAAGGTGGTGCTCCAGTCAGAGGTGGCCAGC CTGCGGCAGAACAACCAGCGGCTGCAGGAGGAGTCGCAGGCCGCCAGCGAGCAGCTGCGCAAGTTTGCG GAGATCTTCTGCAGGGAGAAGAAGGAGCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_015073
Insert Size	5346 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_015073.2
RefSeq Size	8018 bp
RefSeq ORF	5346 bp
Locus ID	23094
UniProt ID	O60292
MW	194.6 kDa
Gene Summary	This gene belongs to the signal induced proliferation associated 1 family of genes, which encode GTPase-activating proteins specific for the GTP-binding protein Rap1. Rap1 has been implicated in regulation of cell adhesion, cell polarity, and organization of the cytoskeleton. Like other members of the family, the protein encoded by this gene contains RapGAP and PDZ domains. In addition, this protein contains a C-terminal leucine zipper domain. This gene is proposed to function in epithelial cell morphogenesis and establishment or maintenance of polarity. Consistently, expression of the protein in cell culture showed localization to cell-cell borders in apical regions, and downregulation of the gene in 3D Caco2 cell culture resulted in abnormal cell polarity and morphogenesis. Allelic variants of this gene have been associated with congenital cataracts in humans. [provided by RefSeq, Feb 2016]

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