NUP188 (NM_015354) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	hNup188; KIAA0169; SANDSTEF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC304297 representing NM_015354. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGCGGCCGCCGGCGGGCCGTGTGTGAGGAGCAGTAGAGAACTGTGGACTATTCTGCTTGGAAGG TCAGCTCTGAGAGAGCTGAGTCAGATTGAGGCAGAACTGAATAAACATTGGCGGCGATTGTTAGAGGGG CTTTCTTACTACAAACCTCCCAGTCCAAGTTCAGCTGAAAAAGTGAAAGCTAATAAAGATGTAGCTTCA CCATTGAAGGAACTGGGTTTAAGAATCAGCAAGTTTTTGGGTCTTGATGAAGAACAGAGTGTGCAGTTA CTCCAGTGTTACCTGCAAGAGGACTACAGGGGTACTCGGGACTCAGTAAAGACAGTACTGCAAGATGAG AGGCAGAGCCAGGCCTTAATCCTGAAGATTGCAGATTATTATTATGAAGAAAGAACCTGTATTCTTCGT TGTGTCTTACACCTTCTCACTTACTTCCAAGATGAAAGACACCCCTATAGGGTTGAATATGCAGACTGT GTTGATAAATTGGAGAAGGAACTAGTTTCAAAATACAGACAGCAGTTCGAAGAGCTTTATAAAACTGAA GCACCAACTTGGGAGACACATGGAAATCTCATGACAGAGCGCCAAGTGTCTCGCTGGTTTGTTCAGTGC CTTCGGGAACAGTCCATGCTGCTAGAAATTATTTTCCTTTATTATGCATACTTTGAGATGGCACCCAGT GACTTACTTGTATTAACCAAGATGTTTAAAGAGCAAGGATTTGGTAGTAGGCAGACCAATAGGCACCTG GTGGATGAGACTATGGATCCTTTTGTAGATCGGATTGGCTACTTCAGTGCCCTCATCCTGGTGGAGGGC ATGGATATCGAGTCCTTGCATAAGTGTGCTTTGGATGACAGAAGAGAACTGCATCAGTTTGCGCAGGAT GGGCTTATTTGTCAGGATATGGACTGTTTAATGTTGACCTTTGGGGACATTCCACATCATGCCCCAGTG CTTTTGGCCTGGGCTCTCCTCCGTCACACTCTGAACCCAGAAGAGACAAGCAGTGTGGTCCGGAAGATA GGTGGCACAGCCATCCAGCTGAATGTGTTTCAGTACTTGACCCGATTGCTCCAGTCCCTTGCCAGTGGG GGAAATGATTGCACCACCAGCACTGCATGCATGTGTGTCTATGGACTGCTCTCTTTCGTTCTGACCTCG TTGGAGCTGCACACCCTGGGCAATCAGCAGGATATAATTGATACAGCATGTGAAGTATTGGCCGACCCT TCTCTTCCGGAACTGTTCTGGGGAACAGAGCCAACTTCTGGCCTTGGGATCATTCTGGACAGTGTGTGT GGAATGTTTCCCCACCTTCTCTCCCCACTCCTGCAACTGCTCCGAGCCCTGGTATCAGGGAAGTCCACA GCCAAAAAGGTGTATAGCTTCTTGGATAAGATGTCTTTCTACAATGAACTTTATAAACACAAGCCTCAT GATGTGATCTCCCATGAAGATGGAACTCTTTGGCGGAGACAAACACCCAAACTCCTTTATCCCCTTGGG GGTCAAACCAACCTTCGCATACCTCAAGGCACTGTGGGCCAAGTAATGTTGGATGATAGGGCATACCTG GTACGCTGGGAATACTCCTATAGCAGCTGGACCCTCTTTACCTGCGAGATTGAAATGTTGCTTCATGTT GTTTCAACTGCAGATGTGATTCAGCACTGCCAGCGAGTCAAACCCATCATTGATCTCGTCCATAAGGTC ATCAGTACAGACCTGTCGATAGCAGACTGTCTCCTGCCCATCACATCTCGCATCTACATGCTGCTGCAG CGGTTAACGACAGTGATCTCCCCACCTGTGGATGTCATTGCTTCTTGTGTCAACTGCTTAACTGTTTTG GCTGCCCGCAATCCAGCAAAGGTCTGGACTGATCTTCGTCACACAGGTTTTTTACCATTTGTGGCCCAT CCTGTCTCCAGCCTGAGTCAGATGATTAGTGCGGAAGGGATGAATGCTGGAGGGTACGGAAACCTCTTG ATGAACAGTGAACAGCCTCAGGGCGAGTATGGGGTTACTATTGCCTTTCTGCGCTTGATCACCACCCTT GTCAAGGGGCAACTTGGTAGTACCCAGAGCCAAGGACTTGTACCCTGTGTAATGTTTGTGCTGAAGGAG ATGCTTCCCAGCTACCATAAGTGGCGCTACAACTCTCATGGAGTGAGGGAACAGATTGGTTGCCTGATC TTGGAGCTGATTCATGCGATACTGAACCTGTGCCACGAGACAGACCTGCACAGCAGTCATACTCCCAGC CTGCAGTTTCTCTGCATCTGCAGCCTGGCATACACAGAAGCAGGACAGACAGTTATCAATATCATGGGC ATTGGCGTGGACACCATTGACATGGTGATGGCTGCTCAGCCTCGAAGTGATGGGGCAGAGGGCCAGGGG CAGGGCCAGCTGCTGATCAAGACAGTGAAACTGGCATTCTCCGTCACCAACAATGTTATTCGGCTGAAA CCTCCTTCTAATGTGGTGTCCCCCCTGGAACAGGCTCTCTCACAACATGGTGCTCATGGAAACAACCTC ATTGCTGTTCTAGCCAAATACATCTACCACAAACATGACCCTGCTTTGCCACGTCTTGCCATTCAGCTG CTGAAACGTCTGGCCACGGTGGCCCCAATGTCAGTGTATGCTTGTCTGGGCAATGATGCGGCTGCCATT CGTGATGCCTTCCTGACCCGATTGCAGAGCAAAATTGAGGACATGCGCATCAAAGTCATGATTCTAGAG TTCCTCACTGTTGCAGTAGAGACCCAGCCAGGCCTCATCGAACTGTTTCTGAACCTGGAAGTTAAGGAT GGCAGTGATGGCTCAAAGGAATTCAGCCTTGGGATGTGGAGCTGTCTCCATGCAGTGCTGGAGCTGATT GATTCCCAACAGCAAGATCGATACTGGTGCCCACCCCTGCTGCATCGTGCCGCCATTGCCTTTTTGCAT GCTCTGTGGCAGGATCGGAGGGACAGTGCCATGCTGGTCCTCCGAACCAAACCCAAGTTTTGGGAAAAT TTAACCAGTCCGCTGTTTGGAACCCTTTCTCCTCCCTCTGAAACATCAGAGCCCAGCATCCTGGAAACC TGTGCCCTAATCATGAAGATAATTTGCTTGGAGATATACTATGTAGTAAAGGGTTCATTAGACCAGTCA TTAAAGGATACACTGAAGAAATTTTCCATCGAGAAACGCTTTGCCTACTGGTCAGGGTATGTCAAGTCA TTGGCAGTTCACGTGGCCGAAACAGAAGGCAGCAGCTGCACCTCCTTGTTAGAGTACCAGATGCTGGTG TCCGCCTGGAGGATGCTTCTCATCATTGCCACCACTCATGCAGATATAATGCACCTGACTGACTCTGTG GTGCGTCGCCAGCTCTTTCTTGACGTGCTTGATGGAACCAAAGCATTACTCCTAGTTCCAGCCTCAGTG AACTGCCTTCGCCTTGGCTCCATGAAGTGCACTCTGCTGCTTATCCTCCTCCGGCAGTGGAAGAGAGAG TTAGGTTCTGTGGATGAAATCCTTGGACCCTTGACGGAGATCCTGGAGGGAGTGCTGCAGGCCGACCAG CAACTCATGGAGAAGACCAAGGCCAAGGTGTTCTCAGCATTCATCACAGTGTTGCAAATGAAGGAGATG AAAGTAAGTGACATCCCCCAGTACTCCCAGCTGGTGCTGAATGTCTGTGAGACCCTCCAAGAGGAAGTG ATTGCACTCTTCGACCAGACCCGCCACAGTCTGGCATTAGGCAGTGCCACAGAGGACAAGGACAGCATG GAGACTGACGACTGTTCTCGGTCCCGGCACAGGGACCAGCGTGATGGGGTGTGTGTCCTGGGCCTGCAC CTGGCCAAGGAGCTGTGTGAGGTAGACGAGGATGGTGACTCCTGGCTGCAGGTAACCCGCAGGCTCCCC ATCCTACCCACCCTCCTCACCACTCTAGAGGTGAGCCTTCGCATGAAGCAGAACCTGCATTTCACTGAG GCCACATTGCATCTGCTCCTCACCCTGGCTCGCACTCAGCAGGGAGCCACAGCAGTGGCTGGAGCTGGC ATCACCCAGAGCATTTGTTTGCCCCTTCTGAGTGTGTACCAGCTGAGCACCAACGGCACAGCACAGACA CCTAGTGCCTCTCGGAAGTCCCTGGATGCCCCCTCTTGGCCAGGAGTCTACCGCCTGTCCATGTCCCTG ATGGAGCAGCTGCTCAAAACTCTGCGCTACAACTTCCTGCCTGAGGCCCTGGACTTCGTGGGTGTCCAC CAGGAGCGGACCTTACAGTGCCTCAACGCAGTGAGGACAGTGCAGAGTCTGGCCTGCCTGGAGGAGGCG GACCACACCGTGGGTTTTATTCTGCAGCTCTCTAACTTCATGAAGGAGTGGCACTTCCACCTGCCTCAG CTCATGCGTGATATCCAGGTCAACCTGGGTTACTTGTGCCAGGCATGTACCTCTCTCCTGCACAGTCGA AAGATGCTGCAGCATTACTTACAGAACAAAAATGGGGATGGCCTCCCCTCAGCTGTTGCCCAGCGAGTC CAGAGGCCACCGTCTGCTGCTTCTGCTGCCCCCTCCTCCTCAAAGCAGCCCGCTGCTGACACAGAGGCA TCAGAGCAGCAGGCCTTGCACACAGTCCAGTATGGCCTTCTCAAGATCCTCAGCAAGACGCTGGCAGCC CTGCGCCACTTCACCCCAGATGTCTGCCAGATTCTGCTGGATCAGTCCCTGGACCTTGCTGAATACAAC TTCCTGTTTGCCCTGAGCTTTACCACTCCCACCTTTGACTCCGAAGTGGCCCCCTCCTTCGGGACCCTT CTGGCCACAGTGAATGTGGCCCTCAACATGCTTGGAGAGCTGGACAAGAAAAAGGAGCCCCTCACCCAG GCAGTGGGGCTCAGCACACAGGCAGAAGGGACCAGGACGTTAAAGTCCCTCCTGATGTTTACCATGGAA AACTGCTTCTACCTGCTCATCTCTCAGGCGATGCGGTACCTTAGGGACCCGGCTGTGCACCCCCGGGAC AAACAGCGGATGAAGCAGGAGCTCAGCTCTGAGTTGAGCACGCTGCTGTCCAGCCTCTCGCGCTACTTC CGCCGGGGAGCCCCCAGCTCCCCTGCCACTGGTGTCCTCCCCTCGCCGCAGGGCAAGTCCACCTCTCTC TCCAAAGCCAGCCCTGAGAGTCAGGAGCCTCTGATCCAGTTGGTGCAGGCGTTTGTCCGGCATATGCAA AGATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_015354
Insert Size	5250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_015354.2
RefSeq Size	5710 bp
RefSeq ORF	5250 bp
Locus ID	23511
UniProt ID	Q5SRE5
MW	196 kDa
Gene Summary	The nuclear pore complex (NPC) is found on the nuclear envelope and forms a gateway that regulates the flow of proteins and RNAs between the cytoplasm and nucleoplasm. The NPC is comprised of approximately 30 distinct proteins collectively known as nucleoporins. Nucleoporins are pore-complex-specific glycoproteins which often have cytoplasmically oriented O-linked N-acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG. However, the nucleoporin protein encoded by this gene does not contain the typical FG repeat sequences found in most vertebrate nucleoporins. This nucleoporin is thought to form part of the scaffold for the central channel of the nuclear pore. [provided by RefSeq, Jan 2013]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.