CWC22 (NM_020943) Human Untagged Clone
CAT＃: SC304872
CWC22 (untagged)-Human CWC22 spliceosome-associated protein homolog (S. cerevisiae) (CWC22)
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EIF4GL; fSAPb; NCM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC304872 representing NM_020943.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAAAGTAGTGTGGCACAGATAAAACCTTCTTCTGGTCATGACAGAAGGGAAAACCTTAATTCATAT CAGAGGAACTCCTCTCCAGAAGACAGATATGAAGAACAAGAACGATCCCCCCGGGATAGAGATTACTTT GATTACAGCAGATCAGACTATGAGCATTCAAGAAGAGGACGTTCTTATGATAGTAGCATGGAGTCACGA AACAGGGACCGAGAAAAACGCAGAGAAAGAGAAAGAGATACGGATCGGAAAAGGTCTCGGAAATCCCCA TCTCCTGGGAGGAGAAACCCAGAAACATCAGTAACTCAGAGTTCCTCTGCTCAGGATGAACCTGCTACA AAGAAAAAGAAAGATGAGCTGGATCCTCTTCTTACTCGCACTGGTGGAGCATATATTCCCCCTGCAAAG CTCAGGATGATGCAGGAACAGATTACAGATAAAAACAGCTTAGCATACCAGAGGATGAGTTGGGAGGCC CTGAAGAAGTCAATTAATGGCCTTATCAACAAAGTCAACATTTCCAACATAAGTATTATTATTCAAGAG CTTCTTCAAGAAAATATAGTTAGAGGAAGAGGACTGCTGTCCAGGTCTGTTTTGCAAGCACAGAGTGCT TCTCCAATCTTCACCCATGTTTATGCAGCATTAGTGGCAATTATCAACTCAAAATTTCCACAAATTGGA GAATTAATCCTCAAAAGGTTAATTCTTAATTTTCGAAAAGGCTATCGAAGAAATGACAAGCAACTTTGC CTGACTGCTTCAAAATTTGTGGCGCATCTTATTAACCAAAATGTGGCACACGAAGTATTATGCTTAGAG ATGCTCACTTTGCTCCTGGAAAGACCAACAGATGATAGCGTTGAAGTAGCTATTGGTTTTCTTAAGGAA TGTGGCCTCAAATTAACACAAGTGTCACCAAGAGGAATCAATGCTATATTTGAACGCCTTCGAAACATT CTGCATGAGTCTGAAATTGACAAAAGAGTTCAATATATGATTGAAGTGATGTTTGCTGTACGGAAAGAT GGATTCAAGGACCACCCCATTATCCTAGAAGGTCTTGATTTGGTGGAAGAAGATGATCAATTCACTCAT ATGCTCCCTCTGGAGGATGACTATAATCCAGAAGATGTTCTTAATGTTTTCAAGATGGATCCTAATTTT ATGGAGAATGAAGAGAAGTACAAAGCTATTAAGAAAGAAATTCTTGATGAGGGAGATACTGACTCGAAC ACAGACCAGGATGCTGGGAGTAGTGAAGAGGACGAGGAAGAAGAAGAGGAAGAGGGAGAAGAAGATGAA GAAGGACAAAAAGTAACTATTCATGACAAAACAGAAATTAACCTGGTCTCATTTCGTCGTACAATTTAT CTTGCTATTCAGTCAAGTTTAGATTTTGAAGAATGTGCTCACAAATTGCTGAAAATGGAGTTTCCTGAA AGCCAAACAAAAGAACTCTGCAACATGATACTTGATTGCTGTGCCCAACAGAGGACATACGAAAAATTT TTTGGCTTATTAGCTGGGCGATTTTGCATGCTAAAGAAAGAGTACATGGAATCCTTTGAAGGTATATTC AAAGAACAGTATGATACCATCCATCGCTTGGAAACAAACAAGTTGCGAAATGTTGCTAAGATGTTTGCT CACCTTTTATACACTGATTCACTTCCATGGAGTGTTCTTGAATGTATAAAACTGAGTGAAGAAACCACT ACATCATCCAGTAGAATTTTTGTCAAAATATTTTTCCAGGAACTGTGTGAATACATGGGTCTTCCTAAA CTTAATGCAAGATTAAAGGATGAAACTCTGCAGCCATTCTTTGAAGGATTATTACCCCGAGATAATCCA AGAAACACTCGGTTTGCCATCAACTTCTTTACTTCTATAGGTCTTGGAGGTTTAACGGATGAACTGCGG GAGCATCTCAAAAATACACCAAAGGTCATTGTGGCGCAGAAACCAGATGTTGAGCAAAATAAATCCTCC CCATCCTCTTCCTCTTCAGCGTCCTCCTCTTCAGAGTCTGACTCATCCGACTCTGATTCTGACAGCAGT GATAGCAGTTCAGAGTCTTCCAGTGAAGAGAGCGACTCTTCATCCATCAGTAGTCATAGCTCTGCCTCA GCTAATGATGTAAGAAAGAAGGGACATGGGAAGACCAGAAGTAAAGAGGTAGATAAATTGATCAGAAAC CAGCAAACAAATGATAGGAAACAAAAAGAAAGAAGACAAGAACACGGGCACCAGGAAACAAGGACTGAG AGAGAAAGAAGGTCAGAAAAACACAGAGATCAAAATTCAAGTGGTTCAAATTGGAGAGATCCTATAACA AAGTACACATCAGACAAAGATGTTCCTTCTGAACGAAATAACTACAGTAGAGTTGCGAATGACAGAGAC CAAGAAATGCATATAGATTTGGAAAATAAGCATGGTGATCCCAAAAAGAAGAGAGGAGAGAGAAGAAAT TCTTTTTCTGAAAATGAGAAGCATACACACCGAATTAAAGACAGTGAAAATTTCAGAAGAAAAGATAGA TCAAAGTCAAAGGAAATGAATAGAAAGCACTCAGGCTCAAGAAGTGATGAAGATAGATATCAAAATGGT GCCGAGAGACGATGGGAAAAATCTAGCAGATACTCTGAACAATCCAGAGAATCAAAGAAAAATCAGGAC CGGCGAAGAGAAAAGTCTCCAGCAAAACAAAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_020943 |
| Insert Size | 2727 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020943.2 |
| RefSeq Size | 3279 bp |
| RefSeq ORF | 2727 bp |
| Locus ID | 57703 |
| UniProt ID | Q9HCG8 |
| MW | 105.5 kDa |
| Gene Summary | Required for pre-mRNA splicing as component of the spliceosome (PubMed:12226669, PubMed:11991638, PubMed:22961380, PubMed:28502770, PubMed:28076346, PubMed:29360106, PubMed:29301961). Promotes exon-junction complex (EJC) assembly (PubMed:22959432, PubMed:22961380). Hinders EIF4A3 from non-specifically binding RNA and escorts it to the splicing machinery to promote EJC assembly on mature mRNAs. Through its role in EJC assembly, required for nonsense-mediated mRNA decay.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210890 | CWC22 (Myc-DDK-tagged)-Human CWC22 spliceosome-associated protein homolog (S. cerevisiae) (CWC22) |
CNY 6648.00 |
|
| RC210890L1 | Lenti ORF clone of Human CWC22 spliceosome-associated protein homolog (S. cerevisiae) (CWC22), Myc-DDK-tagged |
CNY 9030.00 |
|
| RC210890L2 | Lenti ORF clone of Human CWC22 spliceosome-associated protein homolog (S. cerevisiae) (CWC22), mGFP tagged |
CNY 9048.00 |
|
| RC210890L3 | Lenti ORF clone of Human CWC22 spliceosome-associated protein homolog (S. cerevisiae) (CWC22), Myc-DDK-tagged |
CNY 9030.00 |
|
| RC210890L4 | Lenti ORF clone of Human CWC22 spliceosome-associated protein homolog (S. cerevisiae) (CWC22), mGFP tagged |
CNY 9030.00 |
|
| RG210890 | CWC22 (tGFP-tagged) - Human CWC22 spliceosome-associated protein homolog (S. cerevisiae) (CWC22) |
CNY 8248.00 |
