PRDM15 (NM_022115) Human Untagged Clone
CAT＃: SC304996
PRDM15 (untagged)-Human PR domain containing 15 (PRDM15), transcript variant 1
CNY 28980.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C21orf83; PFM15; ZNF298 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC304996 representing NM_022115.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCCCGCCGCCGCCCCCCGGCTTCCGGTGCTGCGCAGTTTCCGGAGCGGATCGCAACCCGGAGTCCG GATCCGATCCCGCTCTGCACATTCCAAAGGCAGCCGCGCGCCGCCCCGGTCCAGCCGCCATGCCGACTG TTCTTTGTTACATTCGCCGGCTGCGGGCACCGTTGGCGATCGGAGTCAAAACCCGGCTGGATTTCCCGG AGCCGCTCCGGGATCGCCCTGCGCGCCGCCCGCCCGCCGGGGTCTTCGCCGCCCCGGCCCGCGGCCCCG CGGCCCCCGCCGCCGGGCGGCGTCGTCGCCGAGGCCCCAGGGGATGTCGTTATCCCCCGCCCTCGGGTA CAGCCCATGCGGGTCGCACGGGGGGGTCCCTGGACCCCCAACCCCGCGTTTAGAGAAGCTGAGTCCTGG TCCCAGATTGGGAACCAGAGGGTCAGTGAGCAGCTCTTGGAAACATCTCTAGGGAATGAGGTGTCCGAC ACTGAGCCACTGAGCCCTGCGAGTGCAGGCCTGCGACGCAATCCTGCCCTACCTCCTGGACCCTTTGCA CAAAACTTTTCCTGGGGGAACCAGGAAAATCTGCCCCCAGCCCTGGGGAAGATTGCGAACGGAGGAGGA ACTGGGGCAGGCAAGGCCGAATGCGGCTATGAAACTGAGTCACACTTGCTAGAGCCGCACGAGATACCT TTGAACGTGAATACACACAAGTTCAGTGACTGTGAGTTTCCATATGAGTTTTGCACGGTCTGCTTTTCA CCCTTCAAGCTGCTGGGGATGAGCGGGGTGGAGGGCGTGTGGAATCAGCATTCAAGAAGTGCCAGCATG CACACTTTCCTAAACCACTCAGCAACGGGCATCAGAGAAGCAGGTTGCAGGAAGGACATGCCGGTGTCA GAGATGGCTGAAGATGGGAGCGAAGAGATCATGTTCATCTGGTGTGAAGACTGCAGCCAGTACCACGAC TCCGAATGTCCCGAGCTGGGCCCAGTGGTCATGGTCAAAGACTCCTTTGTGTTAAGCAGGGCAAGGTCT TGGCCTGCCAGCGGACACGTGCACACCCAGGCGGGGCAGGGGATGCGGGGTTATGAGGACAGGGACAGG GCTGACCCACAGCAGCTTCCAGAAGCAGTCCCTGCAGGCCTGGTGAGGCGGCTCAGTGGGCAGCAGCTG CCCTGCCGTTCCACCCTCACCTGGGGGAGGCTGTGCCACCTGGTGGCCCAGGGCAGGTCATCCCTTCCT CCCAACTTGGAGATCAGACGACTGGAAGATGGAGCCGAGGGGGTGTTCGCCATCACTCAGCTCGTCAAG CGGACACAGTTCGGTCCCTTTGAGTCCAGGAGGGTCGCCAAATGGGAAAAGGAGTCTGCATTTCCCCTG AAGGTGTTCCAGAAGGACGGGCACCCCGTGTGCTTCGACACCTCCAACGAGGATGACTGCAACTGGATG ATGCTGGTGCGGCCAGCGGCGGAGGCCGAGCACCAGAACCTGACGGCCTACCAGCACGGCAGCGACGTG TACTTCACCACCTCCAGAGACATCCCCCCGGGTACCGAGCTGCGCGTGTGGTATGCGGCCTTCTATGCC AAGAAGATGGACAAGCCCATGCTGAAGCAGGCCGGCTCTGGCGTCCACGCTGCAGGCACCCCAGAAAAC AGCGCCCCCGTGGAGTCGGAGCCCAGCCAGTGGGCGTGTAAAGTGTGTTCTGCCACCTTCCTGGAGCTG CAGCTCCTCAATGAACATCTGTTGGGCCACTTAGAACAAGCCAAAAGCCTTCCTCCAGGCAGCCAAAGC GAGGCAGCAGCTCCCGAGAAGGAGCAGGACACACCCCGGGGGGAACCCCCTGCAGTGCCCGAGAGCGAG AATGTTGCCACCAAAGAACAGAAGAAAAAGCCTCGAAGGGGGAGAAAACCCAAAGTGTCCAAAGCTGAG CAGCCTCTAGTCATCGTGGAAGACAAGGAACCCACAGAGCAAGTGGCAGAGATCATTACCGAGGTCCCT CCGGATGAGCCTGTGAGTGCAACGCCAGATGAGCGGATCATGGAGCTGGTTCTGGGGAAGCTGGCCACC ACCACCACTGACACCAGCTCGGTTCCAAAGTTCACCCATCATCAGAATAACACCATCACGCTCAAGAGG AGCTTAATTCTCTCAAGCAGACACGGCATCCGGCGCAAGCTCATCAAACAGCTCGGGGAGCACAAGCGG GTTTACCAGTGCAATATCTGCAGCAAGATCTTCCAGAACAGCAGCAACCTGAGCAGGCACGTGCGCTCG CATGGTGACAAGCTGTTTAAGTGCGAAGAGTGTGCAAAATTGTTCAGCCGCAAAGAGAGCCTAAAGCAG CACGTTTCCTACAAGCACAGCAGGAACGAGGTGGACGGCGAGTACAGGTACCGCTGCGGCACTTGTGAG AAGACCTTCCGCATCGAGAGCGCGCTGGAGTTCCACAACTGCAGGACAGATGACAAGACGTTCCAATGT GAGATGTGTTTCAGATTCTTCTCCACCAACAGCAACCTCTCCAAGCACAAGAAGAAGCACGGCGACAAG AAGTTTGCCTGTGAGGTCTGCAGCAAGATGTTCTACCGCAAGGACGTCATGCTGGACCACCAGCGCCGG CACCTGGAAGGAGTGCGGCGAGTGAAGCGAGAGGACCTGGAGGCCGGTGGGGAGAACCTGGTCCGTTAC AAGAAGGAGCCTTCCGGGTGCCCGGTGTGTGGCAAGGTGTTCTCCTGCCGGAGCAATATGAACAAGCAC CTGCTCACCCACGGCGACAAGAAGTACACCTGCGAGATCTGCGGGCGCAAGTTCTTCCGCGTGGATGTG CTCAGGGACCACATCCATGTCCACTTCAAGGACATCGCGTTGATGGATGACCACCAGAGGGAAGAGTTT ATCGGCAAGATCGGGATCTCCTCGGAAGAAAACGATGACAATTCTGACGAGAGCGCAGACTCGGAGCCT CACAAGTACAGCTGCAAGCGGTGCCAGCTCACCTTCGGCCGGGGGAAGGAGTACCTGAAGCACATCATG GAGGTGCACAAGGAGAAGGGCTATGGCTGCAGCATCTGCAACCGGCGCTTTGCACTGAAGGCCACCTAC CACGCCCACATGGTCATCCACCGTGAAAACCTGCCGGACCCCAACGTGCAGAAGTACATCCACCCCTGC GAGATCTGCGGGCGGATCTTCAACAGCATCGGGAACCTGGAGCGCCACAAGCTCATCCACACAGGTGTG AAGAGCCACGCCTGCGAGCAGTGTGGGAAGTCCTTTGCCAGGAAGGACATGCTGAAGGAGCACATGCGT GTGCACGACAATGTCCGCGAGTACCTGTGTGCCGAGTGTGGGAAAGGCATGAAGACCAAGCACGCGCTG CGCCACCACATGAAGCTGCACAAGGGCATCAAGGAGTACGAGTGCAAGGAGTGCCACCGCAGGTTCGCG CAGAAGGTCAACATGCTCAAGCACTGCAAGCGGCACACGGGGATTAAAGATTTCATGTGTGAATTGTGT GGGAAGACATTCAGCGAGAGGAACACCATGGAGACCCACAAGCTCATCCACACAGTGGGCAAGCAGTGG ACGTGCTCCGTGTGCGACAAGAAGTACGTGACCGAGTACATGCTGCAGAAGCACGTTCAGCTCACACAC GACAAGGTGGAGGCGCAGAGCTGCCAGCTGTGCGGGACCAAGGTGTCCACCAGGGCCTCCATGAGCCGA CACATGCGGCGCAAGCACCCCGAGGTGCTCGCGGTGAGGATCGATGACCTGGACCACCTCCCGGAGACC ACCACCATCGACGCCTCCTCCATTGGCATCGTCCAGCCTGAGCTGACTCTGGAGCAGGAGGATTTGGCC GAAGGGAAGCACGGGAAAGCTGCCAAGCGAAGTCACAAGAGAAAGCAGAAGCCAGAAGAGGAGGCGGGT GCTCCGGTGCCCGAGGACGCCACCTTCAGCGAATACTCAGAGAAAGAGACGGAGTTCACAGGCAGTGTA GGCGACGAGACCAATTCCGCAGTACAGAGCATTCAGCAGGTAGTGGTGACCCTGGGTGACCCAAATGTG ACCACACCATCGAGCTCAGTCGGCTTAACCAACATCACCGTGACCCCCATCACCACTGCGGCCGCGACT CAGTTTACCAATCTCCAGCCGGTGGCCGTGGGGCACCTTACCACCCCTGAACGCCAGTTACAGCTGGAC AACTCAATCCTGACCGTGACCTTTGATACCGTCAGCGGCTCTGCCATGTTGCACAACCGCCAAAATGAC GTCCAGATCCACCCCCAGCCGGAAGCCTCGAACCCACAGTCTGTGGCCCATTTCATCAACCTGACGACC CTGGTCAACTCCATCACGCCCCTGGGGAGCCAGCTTAGTGACCAGCACCCGCTCACGTGGCGGGCAGTG CCCCAGACTGACGTCTTGCCACCCTCGCAGCCGCAGGCACCCCCACAGCAGGCGGCCCAGCCCCAGGTG CAGGCGGAGCAGCAGCAGCAGCAGATGTACAGCTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_022115 |
| Insert Size | 4524 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022115.4 |
| RefSeq Size | 4881 bp |
| RefSeq ORF | 4524 bp |
| Locus ID | 63977 |
| UniProt ID | P57071 |
| MW | 169.3 kDa |
| Gene Summary | Sequence-specific DNA-binding transcriptional regulator. Plays a role as a molecular node in a transcriptional network regulating embryonic development and cell fate decision. Stimulates the expression of upstream key transcriptional activators and repressors of the Wnt/beta-catenin and MAPK/ERK pathways, respectively, that are essential for naive pluripotency and self-renewal maintenance of embryonic stem cells (ESCs). Specifically promotes SPRY1 and RSPO1 transcription activation through recognition and direct binding of a specific DNA sequence in their promoter regions. Involved in early embryo development (By similarity). Plays also a role in induced pluripotent stem cells (iPSCs) reprogramming (PubMed:28740264).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222567 | PRDM15 (Myc-DDK-tagged)-Human PR domain containing 15 (PRDM15), transcript variant 1 |
CNY 10312.00 |
|
| RC222567L3 | Lenti-ORF clone of PRDM15 (Myc-DDK-tagged)-Human PR domain containing 15 (PRDM15), transcript variant 1 |
CNY 16910.00 |
|
| RC222567L4 | Lenti-ORF clone of PRDM15 (mGFP-tagged)-Human PR domain containing 15 (PRDM15), transcript variant 1 |
CNY 16910.00 |
|
| RG222567 | PRDM15 (tGFP-tagged) - Human PR domain containing 15 (PRDM15), transcript variant 1 |
CNY 16530.00 |
